Detailed information    

experimental Experimentally validated

Overview

Name   mecA   Type   Regulator
Locus tag   KW2_RS09195 Genome accession   NC_022369
Coordinates   1926815..1927516 (-) Length   233 a.a.
NCBI ID   WP_011676802.1    Uniprot ID   A0A0M2ZUZ8
Organism   Lactococcus lactis subsp. cremoris KW2     
Function   degradation of ComX   
Competence regulation

Function

MecA, ClpC and ClpP are involved in the proteolytic degradation of ComX, thereby play a significant role in the negative control of competence in strain KW2.


Genomic Context

Location: 1921815..1932516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW2_RS09175 (kw2_1845) - 1921928..1923145 (-) 1218 WP_021037770.1 cysteine desulfurase -
  KW2_RS09180 (kw2_1846) sufD 1923145..1924401 (-) 1257 WP_021037771.1 Fe-S cluster assembly protein SufD -
  KW2_RS09185 (kw2_1847) sufC 1924541..1925311 (-) 771 WP_011835712.1 Fe-S cluster assembly ATPase SufC -
  KW2_RS09190 (kw2_1848) - 1925487..1926812 (-) 1326 WP_011676801.1 MraY family glycosyltransferase -
  KW2_RS09195 (kw2_1849) mecA 1926815..1927516 (-) 702 WP_011676802.1 adaptor protein MecA Regulator
  KW2_RS09200 (kw2_1850) - 1927645..1928379 (-) 735 WP_011835714.1 amino acid ABC transporter ATP-binding protein -
  KW2_RS09205 (kw2_1851) - 1928379..1929065 (-) 687 WP_021037772.1 amino acid ABC transporter permease -
  KW2_RS09210 (kw2_1852) - 1929211..1930119 (-) 909 WP_021037773.1 diacylglycerol/lipid kinase family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  mecA comX negative effect
  comX comGG positive effect
  comX comEA positive effect
  comX ssbB positive effect
  comX comGD positive effect
  comX dprA positive effect
  comX comFA positive effect
  comX coiA positive effect
  comX comGB positive effect
  comX recA positive effect
  comX comFC positive effect
  comX comGE positive effect
  comX comGF positive effect
  comX comEC positive effect
  comX comGA positive effect
  comX comGC positive effect
  clpC comX negative effect
  clpP comX negative effect

Sequence

Protein


Download         Length: 233 a.a.        Molecular weight: 27046.46 Da        Isoelectric Point: 4.1802

>NTDB_id=603 KW2_RS09195 WP_011676802.1 1926815..1927516(-) (mecA) [Lactococcus lactis subsp. cremoris KW2]
MKYEDINENTIKITLSFDDLTDYDIKLSDFFGNQEVIEQFFYELVDELGLENRFGNVGMLTFQIQPFPQGVHMIVHEEAM
LGEGGEIPDDPEEFEELMTGFYNKLNEIGADMARERGITDFKPGLGLPGAKKEEAEHEPDFIYYSIRYDDMMSVLTGIKN
VKFADEESEFYRYDGNFYLVVLDNQKAKGKMHVESTRSRMMEYGEATKMSREFLQEYGECLITTRALDVLRKI

Nucleotide


Download         Length: 702 bp        

>NTDB_id=603 KW2_RS09195 WP_011676802.1 1926815..1927516(-) (mecA) [Lactococcus lactis subsp. cremoris KW2]
ATGAAGTATGAGGATATAAATGAAAACACTATAAAAATCACCTTGTCTTTTGATGATTTGACAGATTATGATATCAAGTT
ATCTGACTTTTTTGGAAATCAAGAAGTCATTGAACAATTTTTCTATGAATTAGTTGATGAACTTGGCTTAGAAAATCGTT
TTGGAAATGTAGGGATGTTAACTTTCCAAATTCAACCCTTCCCACAAGGCGTCCATATGATTGTTCATGAAGAAGCAATG
TTGGGTGAAGGCGGAGAGATTCCAGATGATCCTGAAGAATTTGAAGAATTAATGACTGGTTTTTATAATAAATTAAATGA
AATAGGGGCAGATATGGCGCGCGAGCGAGGAATTACTGATTTTAAACCTGGACTTGGTTTACCAGGGGCTAAAAAAGAAG
AAGCCGAACATGAGCCAGACTTTATATACTACTCTATTCGTTATGATGACATGATGTCTGTCTTGACTGGAATAAAAAAT
GTGAAATTCGCAGATGAAGAGTCAGAATTTTATCGTTATGATGGTAATTTTTATCTTGTTGTTTTAGATAATCAAAAAGC
AAAAGGTAAAATGCATGTTGAAAGCACACGTTCACGGATGATGGAATATGGTGAAGCGACAAAAATGAGTCGAGAATTTT
TGCAGGAGTATGGTGAATGCCTAATCACAACGCGTGCTTTAGACGTTCTTAGAAAAATCTAA

Domains

Predicted by InterproScan.

Negative regulator of genetic competence (MecA) (MecA)

(1-230)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M2ZUZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mecA Lactococcus lactis subsp. lactis strain DGCC12653

96.567

100

0.966

Multiple sequence alignment    



References

[1] Blandine David et al. (2017) Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2. Applied And Environmental Microbiology 83(16):e01074-17. [PMID: 28625996]