Detailed information    

experimental Experimentally validated

Overview

Name   ssbB   Type   Machinery gene
Locus tag   KW2_RS01935 Genome accession   NC_022369
Coordinates   391775..392164 (+) Length   129 a.a.
NCBI ID   WP_021036727.1    Uniprot ID   A0A161TZS1
Organism   Lactococcus lactis subsp. cremoris KW2     
Function   ssDNA binding   
DNA processing

Function

ComX induce the expression of late competence genes.


Genomic Context

Location: 386775..397164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW2_RS01910 (kw2_0386) pepA 387634..388701 (-) 1068 WP_021036726.1 glutamyl aminopeptidase -
  KW2_RS01915 (kw2_0387) - 388804..389088 (+) 285 WP_011675459.1 hypothetical protein -
  KW2_RS01920 (kw2_0388) - 389133..389450 (+) 318 WP_011675460.1 thioredoxin family protein -
  KW2_RS01925 (kw2_0389) ytpR 389556..390182 (+) 627 WP_011675461.1 YtpR family tRNA-binding protein -
  KW2_RS01930 (kw2_0390) nox 390344..391684 (+) 1341 WP_011834457.1 H2O-forming NADH oxidase -
  KW2_RS01935 (kw2_0391) ssbB 391775..392164 (+) 390 WP_021036727.1 single-stranded DNA-binding protein Machinery gene
  KW2_RS01940 (kw2_0392) groES 392283..392567 (+) 285 WP_011834459.1 co-chaperone GroES -
  KW2_RS01945 (kw2_0393) groL 392654..394282 (+) 1629 WP_011675465.1 chaperonin GroEL -
  KW2_RS01950 (kw2_0394) - 394332..395144 (-) 813 WP_021036728.1 MBL fold metallo-hydrolase -
  KW2_RS01960 (kw2_0395) - 395335..396762 (-) 1428 WP_021036729.1 sensor histidine kinase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX ssbB positive effect
  comX comGG positive effect
  comX comEA positive effect
  comX comGD positive effect
  comX dprA positive effect
  comX comFA positive effect
  comX coiA positive effect
  comX comGB positive effect
  comX recA positive effect
  comX comFC positive effect
  comX comGE positive effect
  comX comGF positive effect
  comX comEC positive effect
  comX comGA positive effect
  comX comGC positive effect
  mecA comX negative effect
  clpC comX negative effect
  clpP comX negative effect

Sequence

Protein


Download         Length: 129 a.a.        Molecular weight: 14697.79 Da        Isoelectric Point: 8.3705

>NTDB_id=597 KW2_RS01935 WP_021036727.1 391775..392164(+) (ssbB) [Lactococcus lactis subsp. cremoris KW2]
MNKTMLIGRLTNAPEISKTTNNKSYVRVTLAVNRRFKNEKGEREADFISIILWGKSAETLVSYAKKGSLISVEGEIRTRN
YTDKNEQKHYITEILGLSYDLLESRATLALRESAVKTEELLLEADELPF

Nucleotide


Download         Length: 390 bp        

>NTDB_id=597 KW2_RS01935 WP_021036727.1 391775..392164(+) (ssbB) [Lactococcus lactis subsp. cremoris KW2]
ATGAATAAAACCATGTTGATTGGTCGTTTAACAAACGCACCTGAAATTTCGAAAACAACGAACAATAAATCTTATGTCCG
TGTGACTCTGGCAGTCAATCGCCGCTTCAAAAATGAAAAAGGAGAGCGAGAAGCAGACTTTATCTCGATAATTTTATGGG
GGAAATCAGCAGAAACATTGGTTTCCTATGCTAAAAAAGGAAGTCTTATTTCTGTAGAGGGAGAGATTAGAACTAGAAAT
TATACTGATAAAAATGAACAGAAACACTATATTACAGAAATCTTAGGTTTGAGCTATGATTTATTGGAAAGTCGTGCGAC
TCTTGCCTTAAGAGAAAGCGCTGTAAAGACTGAGGAACTCTTACTTGAAGCTGATGAGCTCCCTTTCTAA

Domains

Predicted by InterProScan.

Single-strand binding protein family (SSB)

(1-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Streptococcus sobrinus strain NIDR 6715-7

61.538

100

0.62
  ssbB/cilA Streptococcus pneumoniae TIGR4

57.692

100

0.581
  ssbB/cilA Streptococcus pneumoniae R6

56.923

100

0.574
  ssbB/cilA Streptococcus mitis NCTC 12261

56.923

100

0.574
  ssbB/cilA Streptococcus mitis SK321

56.923

100

0.574
  ssbB/cilA Streptococcus pneumoniae D39

56.923

100

0.574
  ssbB/cilA Streptococcus pneumoniae Rx1

56.923

100

0.574
  ssbA Streptococcus mutans UA159

55.385

100

0.558
  ssbB Bacillus subtilis subsp. subtilis str. 168

41.905

92.92

0.389
  ssb Latilactobacillus sakei subsp. sakei 23K

41.964

86.822

0.364

Multiple sequence alignment    



References

[1] Blandine David et al. (2017) Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2. Applied And Environmental Microbiology 83(16):e01074-17. [PMID: 28625996]