Detailed information    

experimental Experimentally validated

Overview

Name   ssbA   Type   Machinery gene
Locus tag   SMU_RS08940 Genome accession   NC_004350
Coordinates   1843662..1844057 (-) Length   131 a.a.
NCBI ID   WP_002263454.1    Uniprot ID   Q8DS68
Organism   Streptococcus mutans UA159     
Function   ssDNA binding   
DNA processing

Function

Deletion of the genes for recombinase A, recA, DNA processing protein, dprA and single-stranded DNA-binding protein, ssbA, produced results comparable with those from other bacteria, supporting the contention that these proteins have similar functions in S. mutans competence.


Genomic Context

Location: 1838662..1849057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMU_RS08915 - 1838712..1838915 (-) 204 WP_002279759.1 hypothetical protein -
  SMU_RS08920 (SMU.1963c) - 1838982..1840289 (-) 1308 WP_002263458.1 extracellular solute-binding protein -
  SMU_RS08925 (SMU.1964c) - 1840282..1840962 (-) 681 WP_002263457.1 response regulator transcription factor -
  SMU_RS08930 (SMU.1965c) - 1840962..1842284 (-) 1323 WP_002263456.1 sensor histidine kinase -
  SMU_RS08935 (SMU.1966c) - 1842284..1843246 (-) 963 WP_002263455.1 sugar ABC transporter substrate-binding protein -
  SMU_RS08940 (SMU.1967) ssbA 1843662..1844057 (-) 396 WP_002263454.1 single-stranded DNA-binding protein Machinery gene
  SMU_RS08945 (SMU.1968c) - 1844144..1844500 (-) 357 WP_002263453.1 rhodanese-like domain-containing protein -
  SMU_RS08950 (SMU.1969c) - 1844500..1844922 (-) 423 WP_002263452.1 MarR family winged helix-turn-helix transcriptional regulator -
  SMU_RS08955 (SMU.1970c) ytpR 1845020..1845646 (-) 627 WP_002263451.1 YtpR family tRNA-binding protein -
  SMU_RS08960 (SMU.1971c) - 1845643..1845972 (-) 330 WP_002263450.1 thioredoxin family protein -
  SMU_RS08965 (SMU.1972c) - 1845950..1846258 (-) 309 WP_002263449.1 DUF4651 domain-containing protein -
  SMU_RS08970 (SMU.1973) pepA 1846318..1847385 (+) 1068 WP_002263448.1 glutamyl aminopeptidase -
  SMU_RS08975 (SMU.1974) proC 1847441..1848211 (+) 771 WP_002263447.1 pyrroline-5-carboxylate reductase -
  SMU_RS08980 (SMU.1975c) - 1848296..1848958 (-) 663 WP_002263446.1 type II CAAX endopeptidase family protein -

Sequence

Protein


Download         Length: 131 a.a.        Molecular weight: 14841.90 Da        Isoelectric Point: 6.4855

>NTDB_id=409 SMU_RS08940 WP_002263454.1 1843662..1844057(-) (ssbA) [Streptococcus mutans UA159]
MYNKVILIGRLVATPEMVKTPKDKSVTRVTLAVNRRFKSQDGEKQADFVNVVLWGRLAETIASYGSKGSLISLDGELRTR
KYEKDGVTHFVTEVLGHSFQLLESRAQRAMRENNSENDLADLVLEEEELPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=409 SMU_RS08940 WP_002263454.1 1843662..1844057(-) (ssbA) [Streptococcus mutans UA159]
ATGTATAATAAAGTTATTTTGATTGGTCGTTTAGTTGCGACACCTGAAATGGTAAAAACACCAAAGGATAAGTCTGTTAC
GCGCGTGACACTGGCTGTCAATCGGCGATTTAAAAGTCAAGATGGGGAAAAGCAGGCTGATTTTGTCAATGTTGTTCTTT
GGGGCAGATTGGCAGAAACAATAGCTTCCTATGGTAGTAAGGGAAGTCTCATCTCTCTTGATGGAGAATTGCGTACCCGT
AAATATGAAAAAGATGGCGTCACTCATTTTGTGACTGAAGTTTTGGGACACTCCTTTCAATTACTGGAAAGTCGTGCTCA
ACGTGCTATGCGTGAAAATAATTCTGAAAATGATTTGGCCGATTTGGTTCTAGAAGAGGAGGAACTCCCTTTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(3-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DS68

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Streptococcus sobrinus strain NIDR 6715-7

78.626

100

0.786
  ssbB/cilA Streptococcus pneumoniae TIGR4

75.573

100

0.756
  ssbB/cilA Streptococcus mitis SK321

74.809

100

0.748
  ssbB/cilA Streptococcus pneumoniae R6

74.809

100

0.748
  ssbB/cilA Streptococcus pneumoniae D39

74.809

100

0.748
  ssbB/cilA Streptococcus pneumoniae Rx1

74.809

100

0.748
  ssbB/cilA Streptococcus mitis NCTC 12261

73.282

100

0.733
  ssbB Lactococcus lactis subsp. cremoris KW2

55.385

100

0.558
  ssbB Bacillus subtilis subsp. subtilis str. 168

44.34

93.805

0.416
  ssbA Bacillus subtilis subsp. subtilis str. 168

50

80.916

0.405

Multiple sequence alignment    



References

[1] R E Eaton et al. (2010) Deletion of competence-induced genes over-expressed in biofilms caused transformation deficiencies in Streptococcus mutans. Molecular Oral Microbiology 25(6):406-17. [PMID: 21040514]