Detailed information    

experimental Experimentally validated

Overview

Name   ssbB/cilA   Type   Machinery gene
Locus tag   SMSK321_RS08825 Genome accession   NZ_AEDT01000026
Coordinates   93011..93406 (+) Length   131 a.a.
NCBI ID   WP_000282440.1    Uniprot ID   A0A0F2D6P4
Organism   Streptococcus mitis SK321     
Function   ssDNA binding   
DNA processing

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 88011..98406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMSK321_RS08800 (SMSK321_1188) - 89563..90638 (+) 1076 Protein_96 N-acetylmuramoyl-L-alanine amidase family protein -
  SMSK321_RS08805 (SMSK321_1189) - 90875..91174 (+) 300 WP_001815024.1 DUF4651 domain-containing protein -
  SMSK321_RS08810 (SMSK321_1190) - 91171..91488 (+) 318 WP_000615778.1 thioredoxin family protein -
  SMSK321_RS08815 (SMSK321_1191) ytpR 91504..92130 (+) 627 WP_000578276.1 YtpR family tRNA-binding protein -
  SMSK321_RS08820 (SMSK321_1192) - 92173..92934 (+) 762 WP_000042283.1 SDR family oxidoreductase -
  SMSK321_RS08825 (SMSK321_1193) ssbB/cilA 93011..93406 (+) 396 WP_000282440.1 single-stranded DNA-binding protein Machinery gene
  SMSK321_RS08830 (SMSK321_1194) groES 93562..93846 (+) 285 WP_000917335.1 co-chaperone GroES -
  SMSK321_RS08835 (SMSK321_1195) groL 93862..95484 (+) 1623 WP_000031569.1 chaperonin GroEL -
  SMSK321_RS08840 (SMSK321_1196) - 95622..96389 (+) 768 WP_000567573.1 epoxyqueuosine reductase QueH -
  SMSK321_RS08845 (SMSK321_1197) - 96789..97562 (+) 774 WP_000566042.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 131 a.a.        Molecular weight: 14877.83 Da        Isoelectric Point: 5.9409

>NTDB_id=526 SMSK321_RS08825 WP_000282440.1 93011..93406(+) (ssbB/cilA) [Streptococcus mitis SK321]
MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGEREADFVNMVLWGRLAETLASYATKGSLISVDGELRTR
RFEKNGQMNYVAEVLVTGFQLLESRAQRAMRENNAGQDLADLVLEEEELPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=526 SMSK321_RS08825 WP_000282440.1 93011..93406(+) (ssbB/cilA) [Streptococcus mitis SK321]
ATGTATAATAAAGTTATCTTAATCGGACGCTTGACGTCTACACCAGAATTGCACAAAACCAACAATGACAAGTCAGTTGC
GCGAGCAACTATCGCTGTGAACCGTCGTTACAAAGACCAAAATGGGGAACGTGAAGCTGATTTTGTCAATATGGTCCTTT
GGGGCAGGCTAGCAGAAACCTTGGCAAGCTACGCAACTAAAGGTAGCCTCATTTCAGTGGATGGAGAATTACGTACCCGT
CGTTTTGAGAAAAATGGTCAGATGAACTACGTGGCCGAAGTCCTCGTGACAGGATTCCAACTCTTGGAAAGTCGTGCCCA
ACGTGCTATGCGTGAAAATAATGCAGGCCAAGATTTGGCGGATTTGGTTTTGGAAGAGGAAGAATTGCCATTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(3-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F2D6P4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB/cilA Streptococcus pneumoniae TIGR4

99.237

100

0.992
  ssbB/cilA Streptococcus pneumoniae D39

98.473

100

0.985
  ssbB/cilA Streptococcus pneumoniae Rx1

98.473

100

0.985
  ssbB/cilA Streptococcus pneumoniae R6

98.473

100

0.985
  ssbB/cilA Streptococcus mitis NCTC 12261

96.947

100

0.969
  ssbA Streptococcus mutans UA159

74.809

100

0.748
  ssbB Streptococcus sobrinus strain NIDR 6715-7

70.229

100

0.702
  ssbB Lactococcus lactis subsp. cremoris KW2

56.923

100

0.574
  ssbB Bacillus subtilis subsp. subtilis str. 168

43.519

95.575

0.416
  ssbA Bacillus subtilis subsp. subtilis str. 168

50.943

80.916

0.412
  ssb Latilactobacillus sakei subsp. sakei 23K

46.903

86.26

0.405

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]