Detailed information    

experimental Experimentally validated

Overview

Name   ssbA   Type   Machinery gene
Locus tag   BSU_40900 Genome accession   NC_000964
Coordinates   4198886..4199404 (-) Length   172 a.a.
NCBI ID   NP_391970.1    Uniprot ID   P37455
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   ssDNA binding   
DNA processing

Function

DprA-SsbA contributes to the formation of an active RecA filament and directly antagonizes the inhibitory effects of RecX during natural transformation. SsbA and RecO (or SsbA, RecO and RecR in vivo) are crucial for RecA activation for both, AddAB and RecJ-RecQ (RecS) recombinational repair pathways.


Genomic Context

Location: 4193886..4204404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_40840 (BSU40840) yyaJ 4194389..4195744 (+) 1356 NP_391964.2 putative transporter -
  BSU_40850 (BSU40850) maa 4195778..4196332 (-) 555 NP_391965.1 maltose O-acetyltransferase -
  BSU_40860 (BSU40860) yyaH 4196350..4196730 (-) 381 NP_391966.1 putative C-S lyase -
  BSU_40870 (BSU40870) ccpB 4196786..4197721 (-) 936 NP_391967.1 transcriptional repressor of carbon supply (LacI family) -
  BSU_40880 (BSU40880) exoA 4197780..4198538 (-) 759 NP_391968.1 apurinic/apyrimidinic endonuclease -
  BSU_40890 (BSU40890) rpsR 4198603..4198842 (-) 240 NP_391969.2 ribosomal protein S18 -
  BSU_40900 (BSU40900) ssbA 4198886..4199404 (-) 519 NP_391970.1 single-strand DNA-binding protein Machinery gene
  BSU_40910 (BSU40910) rpsF 4199445..4199732 (-) 288 NP_391971.1 ribosomal protein S6 (BS9) -
  BSU_40920 (BSU40920) engD 4199843..4200943 (-) 1101 NP_391972.1 potassium-dependent informational ATPase interacting with 70S ribosome; ROS stress regulator -
  BSU_40930 (BSU40930) yyaE 4201070..4203073 (-) 2004 NP_391973.1 putative oxidoreductase (molybdoenzyme) -
  BSU_40939 (BSU40939) yyzM 4203124..4203330 (-) 207 YP_003097803.1 putative nucleic acid binding protein -

Sequence

Protein


Download         Length: 172 a.a.        Molecular weight: 18742.31 Da        Isoelectric Point: 4.7621

>NTDB_id=113 BSU_40900 NP_391970.1 4198886..4199404(-) (ssbA) [Bacillus subtilis subsp. subtilis str. 168]
MLNRVVLVGRLTKDPELRYTPNGAAVATFTLAVNRTFTNQSGEREADFINCVTWRRQAENVANFLKKGSLAGVDGRLQTR
NYENQQGQRVFVTEVQAESVQFLEPKNGGGSGSGGYNEGNSGGGQYFGGGQNDNPFGGNQNNQRRNQGNSFNDDPFANDG
KPIDISDDDLPF

Nucleotide


Download         Length: 519 bp        

>NTDB_id=113 BSU_40900 NP_391970.1 4198886..4199404(-) (ssbA) [Bacillus subtilis subsp. subtilis str. 168]
ATGCTTAACCGAGTTGTATTAGTCGGAAGACTGACAAAAGACCCAGAGCTTCGTTATACGCCAAACGGTGCGGCTGTTGC
TACGTTTACTCTTGCTGTGAATCGTACATTTACGAACCAGTCCGGAGAACGTGAGGCCGATTTCATTAATTGTGTCACTT
GGAGAAGACAAGCCGAAAACGTTGCAAACTTCTTGAAAAAAGGAAGCCTTGCAGGCGTAGATGGCCGTTTACAAACAAGA
AACTATGAAAACCAGCAAGGACAGCGTGTCTTCGTGACAGAGGTCCAAGCTGAAAGTGTTCAATTTCTTGAGCCGAAAAA
CGGCGGCGGTTCTGGTTCAGGTGGATACAACGAAGGAAACAGCGGCGGAGGCCAGTACTTTGGCGGAGGCCAAAATGATA
ATCCATTTGGGGGAAATCAAAACAACCAGAGACGCAATCAGGGGAACAGCTTTAATGATGACCCATTTGCCAACGACGGC
AAACCGATTGACATCTCGGATGATGATCTTCCATTCTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 6BHW
  PDB 6BHX

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Bacillus subtilis subsp. subtilis str. 168

64.151

93.805

0.602
  ssb Latilactobacillus sakei subsp. sakei 23K

58.192

100

0.599
  ssbB/cilA Streptococcus pneumoniae TIGR4

51.887

80.916

0.42
  ssbB/cilA Streptococcus pneumoniae D39

50.943

80.916

0.412
  ssbB/cilA Streptococcus pneumoniae Rx1

50.943

80.916

0.412
  ssbB/cilA Streptococcus pneumoniae R6

50.943

80.916

0.412
  ssbB/cilA Streptococcus mitis SK321

50.943

80.916

0.412
  ssbA Streptococcus mutans UA159

50

80.916

0.405
  ssbB/cilA Streptococcus mitis NCTC 12261

50

80.916

0.405
  ssbB Streptococcus sobrinus strain NIDR 6715-7

49.057

80.916

0.397
  ssb Glaesserella parasuis strain SC1401

35.519

100

0.378

Multiple sequence alignment    



References

[1] Begoña Carrasco et al. (2022) PcrA Dissociates RecA Filaments and the SsbA and RecO Mediators Counterbalance Such Activity. Frontiers in Molecular Biosciences 9:836211. [PMID: 35223992]
[2] Shimin Le et al. (2017) Bacillus subtilis RecA with DprA-SsbA antagonizes RecX function during natural transformation. Nucleic Acids Research 45(15):8873-8885. [PMID: 28911099]
[3] Begoña Carrasco et al. (2015) Bacillus subtilis RecO and SsbA are crucial for RecA-mediated recombinational DNA repair. Nucleic Acids Research 43(12):5984-97. [PMID: 26001966]
[4] Tribhuwan Yadav et al. (2014) Roles of Bacillus subtilis DprA and SsbA in RecA-mediated genetic recombination. The Journal of Biological Chemistry 289(40):27640-52. [PMID: 25138221]
[5] Tribhuwan Yadav et al. (2013) Bacillus subtilis DprA recruits RecA onto single-stranded DNA and mediates annealing of complementary strands coated by SsbB and SsbA. The Journal of Biological Chemistry 288(31):22437-50. [PMID: 23779106]
[6] Begoña Carrasco et al. (2008) Bacillus subtilis SsbA and dATP regulate RecA nucleation onto single-stranded DNA. DNA Repair 7(6):990-6. [PMID: 18472308]
[7] Candela Manfredi et al. (2008) Bacillus subtilis RecO nucleates RecA onto SsbA-coated single-stranded DNA. The Journal of Biological Chemistry 283(36):24837-47. [PMID: 18599486]