Detailed information    

experimental Experimentally validated

Overview

Name   ssbB/cilA   Type   Machinery gene
Locus tag   SM12261_RS01980 Genome accession   NZ_CP028414
Coordinates   367697..368092 (+) Length   131 a.a.
NCBI ID   WP_000282464.1    Uniprot ID   -
Organism   Streptococcus mitis NCTC 12261     
Function   ssDNA binding   
DNA processing

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 362697..373092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS01950 (SM12261_0377) - 364285..365358 (+) 1074 WP_161969979.1 N-acetylmuramoyl-L-alanine amidase family protein -
  SM12261_RS01960 (SM12261_0378) - 365563..365862 (+) 300 WP_001013977.1 DUF4651 domain-containing protein -
  SM12261_RS01965 (SM12261_0379) - 365859..366176 (+) 318 WP_000615777.1 thioredoxin family protein -
  SM12261_RS01970 (SM12261_0380) ytpR 366192..366818 (+) 627 WP_000578319.1 YtpR family tRNA-binding protein -
  SM12261_RS01975 (SM12261_0381) - 366860..367621 (+) 762 WP_000165331.1 SDR family NAD(P)-dependent oxidoreductase -
  SM12261_RS01980 (SM12261_0382) ssbB/cilA 367697..368092 (+) 396 WP_000282464.1 single-stranded DNA-binding protein Machinery gene
  SM12261_RS01985 (SM12261_0383) groES 368248..368532 (+) 285 WP_000917337.1 co-chaperone GroES -
  SM12261_RS01990 (SM12261_0384) groL 368548..370170 (+) 1623 WP_000031580.1 chaperonin GroEL -
  SM12261_RS01995 (SM12261_0385) - 370308..371075 (+) 768 WP_000567554.1 epoxyqueuosine reductase QueH -
  SM12261_RS02000 (SM12261_0386) - 371146..371679 (-) 534 WP_000775316.1 DUF402 domain-containing protein -
  SM12261_RS02005 (SM12261_0387) recX 371768..372544 (-) 777 WP_049499824.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 131 a.a.        Molecular weight: 14883.85 Da        Isoelectric Point: 5.9409

>NTDB_id=498 SM12261_RS01980 WP_000282464.1 367697..368092(+) (ssbB/cilA) [Streptococcus mitis NCTC 12261]
MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGEREADFVNMVLWGKLAESLASYATKGSLISVDGELRTR
RFEKNGQMNYVTEVLVTGFQLLESRAQRAMRENNAGQDLADLVLEEEELPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=498 SM12261_RS01980 WP_000282464.1 367697..368092(+) (ssbB/cilA) [Streptococcus mitis NCTC 12261]
ATGTATAATAAAGTTATCATGATTGGGCGTTTGACGTCTACACCAGAATTGCACAAAACCAACAATGACAAGTCAGTAGC
GCGAGCAACTATCGCTGTGAACCGTCGTTACAAAGACCAAAATGGGGAGCGCGAAGCTGACTTTGTCAATATGGTTCTAT
GGGGCAAACTAGCAGAAAGCTTGGCCAGCTACGCAACTAAAGGTAGTCTTATCTCTGTGGATGGAGAACTTCGTACCCGT
CGTTTTGAGAAAAATGGTCAGATGAACTATGTGACTGAAGTCCTTGTGACAGGATTCCAACTCTTGGAAAGTCGTGCTCA
ACGTGCCATGCGTGAAAATAATGCAGGACAAGATTTGGCGGATTTGGTTTTGGAAGAGGAAGAATTGCCATTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(3-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB/cilA Streptococcus pneumoniae R6

98.473

100

0.985
  ssbB/cilA Streptococcus pneumoniae D39

98.473

100

0.985
  ssbB/cilA Streptococcus pneumoniae Rx1

98.473

100

0.985
  ssbB/cilA Streptococcus pneumoniae TIGR4

97.71

100

0.977
  ssbB/cilA Streptococcus mitis SK321

96.947

100

0.969
  ssbA Streptococcus mutans UA159

73.282

100

0.733
  ssbB Streptococcus sobrinus strain NIDR 6715-7

71.756

100

0.718
  ssbB Lactococcus lactis subsp. cremoris KW2

56.923

100

0.574
  ssbB Bacillus subtilis subsp. subtilis str. 168

44.444

95.575

0.425
  ssb Latilactobacillus sakei subsp. sakei 23K

47.788

86.26

0.412
  ssbA Bacillus subtilis subsp. subtilis str. 168

50

80.916

0.405

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]