Detailed information    

experimental Experimentally validated

Overview

Name   ssbB   Type   Machinery gene
Locus tag   BSU_36310 Genome accession   NC_000964
Coordinates   3740206..3740547 (-) Length   113 a.a.
NCBI ID   NP_391512.2    Uniprot ID   C0SPB6
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   ssDNA binding   
DNA processing

Function

The ssbB gene product, SsbB, is essential for the stabilization of ssDNA during the competence process. SsbB binds to ssDNA and protects it from degradation, facilitating the efficient uptake and integration of exogenous DNA into the bacterial chromosome.


Genomic Context

Location: 3735206..3745547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_36280 (BSU36280) hepA 3735449..3738217 (+) 2769 NP_391509.1 ATPase involved in RNA remodelling DNA recombination and repair -
  BSU_36290 (BSU36290) ywpJ 3738343..3739200 (-) 858 NP_391510.1 phosphatase of unidentified specificity (possibly promiscuous) -
  BSU_36300 (BSU36300) glcR 3739206..3739982 (-) 777 NP_391511.1 transcriptional regulator (glucose repression of catabolic operons) -
  BSU_36310 (BSU36310) ssbB 3740206..3740547 (-) 342 NP_391512.2 single-strand DNA-binding protein Machinery gene
  BSU_36320 (BSU36320) ywpG 3740624..3741007 (-) 384 NP_391513.1 interaction partner of DynA -
  BSU_36330 (BSU36330) ywpF 3741182..3741592 (+) 411 NP_391514.1 hypothetical protein -
  BSU_36340 (BSU36340) ywpE 3741732..3742040 (-) 309 NP_391515.1 putative sortase -
  BSU_36350 (BSU36350) ywpD 3742384..3743220 (+) 837 NP_391516.1 putative two-component sensor histidine kinase -
  BSU_36360 (BSU36360) mscL 3743267..3743659 (-) 393 NP_391517.1 large conductance mechanosensitive channel protein -
  BSU_36370 (BSU36370) fabZ 3743732..3744157 (-) 426 NP_391518.2 beta-hydroxyacyl-[acyl carrier protein] dehydratase -
  BSU_36380 (BSU36380) rapD 3744349..3745413 (+) 1065 NP_391519.1 response regulator aspartate phosphatase -

Sequence

Protein


Download         Length: 113 a.a.        Molecular weight: 12520.15 Da        Isoelectric Point: 6.9498

>NTDB_id=112 BSU_36310 NP_391512.2 3740206..3740547(-) (ssbB) [Bacillus subtilis subsp. subtilis str. 168]
MFNQVMLVGRLTKDPDLRYTSAGAAVAHVTLAVNRSFKNASGEIEADYVNCTLWRKTAENTALYCQKGSLVGVSGRIQTR
SYENEEGVNVYVTEVLADTVRFMDPKPREKAAD

Nucleotide


Download         Length: 342 bp        

>NTDB_id=112 BSU_36310 NP_391512.2 3740206..3740547(-) (ssbB) [Bacillus subtilis subsp. subtilis str. 168]
ATGTTCAATCAGGTCATGCTTGTCGGACGTCTTACAAAAGATCCTGATCTTCGCTACACTTCCGCCGGTGCGGCAGTTGC
ACATGTTACGCTCGCGGTGAACCGCAGCTTCAAGAATGCTTCAGGTGAAATCGAAGCTGATTACGTCAATTGCACACTTT
GGAGAAAAACAGCTGAAAACACGGCGTTGTATTGCCAAAAAGGTTCTCTCGTCGGCGTAAGCGGACGGATTCAGACAAGA
AGCTATGAAAACGAGGAAGGCGTTAACGTGTATGTAACAGAAGTGTTGGCTGACACTGTTCGTTTTATGGACCCTAAACC
CCGGGAAAAAGCTGCTGATTGA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 3VDY

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

64.151

93.805

0.602
  ssb Latilactobacillus sakei subsp. sakei 23K

59.434

93.805

0.558
  ssbB Streptococcus sobrinus strain NIDR 6715-7

45.37

95.575

0.434
  ssbB/cilA Streptococcus mitis NCTC 12261

44.444

95.575

0.425
  ssbB/cilA Streptococcus pneumoniae TIGR4

44.444

95.575

0.425
  ssbB/cilA Streptococcus pneumoniae Rx1

43.519

95.575

0.416
  ssbB/cilA Streptococcus pneumoniae D39

43.519

95.575

0.416
  ssbB/cilA Streptococcus pneumoniae R6

43.519

95.575

0.416
  ssbB/cilA Streptococcus mitis SK321

43.519

95.575

0.416
  ssbA Streptococcus mutans UA159

44.34

93.805

0.416
  ssbB Lactococcus lactis subsp. cremoris KW2

41.905

92.92

0.389

Multiple sequence alignment    



References

[1] Tribhuwan Yadav et al. (2014) Roles of Bacillus subtilis DprA and SsbA in RecA-mediated genetic recombination. The Journal of Biological Chemistry 289(40):27640-52. [PMID: 25138221]
[2] Tribhuwan Yadav et al. (2013) Bacillus subtilis DprA recruits RecA onto single-stranded DNA and mediates annealing of complementary strands coated by SsbB and SsbA. The Journal of Biological Chemistry 288(31):22437-50. [PMID: 23779106]
[3] Tribhuwan Yadav et al. (2012) Genetic recombination in Bacillus subtilis: a division of labor between two single-strand DNA-binding proteins. Nucleic Acids Research 40(12):5546-59. [PMID: 22373918]
[4] Naomi Kramer et al. (2007) Multiple interactions among the competence proteins of Bacillus subtilis. Molecular Microbiology 65(2):454-64. [PMID: 17630974]
[5] Ivan Mijakovic et al. (2006) Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. Nucleic Acids Research 34(5):1588-96. [PMID: 16549871]
[6] Cordula Lindner et al. (2004) Differential expression of two paralogous genes of Bacillus subtilis encoding single-stranded DNA binding protein. Journal of Bacteriology 186(4):1097-105. [PMID: 14762004]