Detailed information    

experimental Experimentally validated

Overview

Name   coiA   Type   Machinery gene
Locus tag   KW2_RS08500 Genome accession   NC_022369
Coordinates   1799423..1800406 (-) Length   327 a.a.
NCBI ID   WP_021037660.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. cremoris KW2     
Function   homologous recombination   
Homologous recombination

Function

ComX induce the expression of late competence genes.


Genomic Context

Location: 1794423..1805406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW2_RS08485 (kw2_1702) - 1795774..1796700 (-) 927 WP_014572243.1 peptidyl-prolyl cis-trans isomerase -
  KW2_RS08490 (kw2_1703) - 1796856..1797539 (-) 684 WP_021037658.1 O-methyltransferase -
  KW2_RS08495 (kw2_1704) pepF 1797542..1799347 (-) 1806 WP_021037659.1 oligoendopeptidase F Regulator
  KW2_RS08500 (kw2_1705) coiA 1799423..1800406 (-) 984 WP_021037660.1 competence protein CoiA Machinery gene
  KW2_RS08505 (kw2_1706) - 1800481..1801566 (-) 1086 WP_014735114.1 YdcF family protein -
  KW2_RS08510 (kw2_1707) - 1801720..1802568 (-) 849 WP_021037661.1 alpha/beta hydrolase -
  KW2_RS08515 (kw2_1708) - 1802777..1804198 (-) 1422 WP_011676727.1 NCS2 family permease -
  KW2_RS08520 (kw2_1709) - 1804504..1805340 (+) 837 WP_021037662.1 Cof-type HAD-IIB family hydrolase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX coiA positive effect
  comX comGG positive effect
  comX comEA positive effect
  comX ssbB positive effect
  comX comGD positive effect
  comX dprA positive effect
  comX comFA positive effect
  comX comGB positive effect
  comX recA positive effect
  comX comFC positive effect
  comX comGE positive effect
  comX comGF positive effect
  comX comEC positive effect
  comX comGA positive effect
  comX comGC positive effect
  mecA comX negative effect
  clpC comX negative effect
  clpP comX negative effect

Sequence

Protein


Download         Length: 327 a.a.        Molecular weight: 39207.77 Da        Isoelectric Point: 9.8315

>NTDB_id=598 KW2_RS08500 WP_021037660.1 1799423..1800406(-) (coiA) [Lactococcus lactis subsp. cremoris KW2]
MRTAIDENNKIINLLELDRKELTGKFYCPSCHSELLIKNGQIKVLHFAHKSLKSCNLWLENESEQHLGLKKILYQWFKRT
DKVEIERYLPELNQRPDLLVNDKIAIEIQCSHLSIKRLKERTENYKTHGFKVLWLMGKDLWLAEQVTELQKNLVYFSENR
GFFYWELDFQRKKLRLKSLIHEDLRGRIICLQEEIPFGKGRLIAHLRLPYLAQKLVKIPTFKDSKLSSFIRQQLYYQSPK
WMKIQEKYYQKGENLLTKKFEGPYIAPLGLNLLENFTDEMTITTFTQIDQNVKLYYENFLINFQRNSLEMLYPPRSYAIM
GKQKKEK

Nucleotide


Download         Length: 984 bp        

>NTDB_id=598 KW2_RS08500 WP_021037660.1 1799423..1800406(-) (coiA) [Lactococcus lactis subsp. cremoris KW2]
ATGCGGACAGCAATTGATGAAAATAATAAAATAATAAACTTACTGGAATTAGACCGCAAAGAATTGACCGGAAAATTTTA
TTGTCCTTCTTGTCATTCAGAGCTACTTATTAAAAATGGGCAGATAAAAGTTCTTCATTTTGCCCATAAATCTCTAAAGA
GCTGCAATTTATGGTTGGAAAATGAGTCAGAACAGCATTTAGGTTTGAAAAAAATATTATATCAGTGGTTTAAAAGAACT
GACAAAGTCGAAATTGAACGCTATTTGCCCGAACTTAACCAAAGACCAGATTTATTAGTAAATGACAAAATCGCTATTGA
GATACAATGCTCTCATTTATCTATTAAGCGTCTCAAAGAACGTACAGAAAATTATAAGACGCATGGCTTTAAAGTGCTTT
GGTTGATGGGAAAGGATTTATGGCTGGCTGAACAGGTGACAGAACTTCAAAAAAATTTAGTTTATTTCTCGGAAAATAGA
GGATTCTTTTATTGGGAGTTAGATTTTCAGAGGAAAAAATTAAGATTAAAATCTCTTATTCATGAGGATTTACGTGGCAG
AATCATTTGCTTACAAGAGGAAATTCCTTTTGGAAAAGGACGACTTATTGCGCATCTACGCTTACCTTATTTGGCTCAAA
AACTGGTGAAAATTCCTACTTTTAAAGATTCTAAACTTTCTAGTTTTATTCGTCAGCAATTATACTATCAATCACCAAAA
TGGATGAAAATTCAAGAAAAATATTATCAAAAAGGAGAAAATTTACTGACGAAAAAATTTGAGGGTCCATACATTGCTCC
ACTAGGTTTAAATTTACTAGAGAATTTTACTGACGAAATGACAATCACAACTTTTACTCAGATTGATCAAAATGTTAAGC
TTTACTATGAAAATTTTTTAATAAATTTCCAAAGAAATAGCCTTGAAATGCTCTATCCTCCCCGTTCCTATGCTATAATG
GGAAAGCAGAAGAAGGAAAAATAA

Domains

Predicted by InterproScan.

Competence protein CoiA-like family (CoiA_nuc)

(1-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  coiA Streptococcus mitis NCTC 12261

44.099

100

0.448
  coiA Streptococcus pneumoniae TIGR4

42.593

100

0.435
  coiA Streptococcus pneumoniae R6

42.593

100

0.435
  coiA Streptococcus pneumoniae D39

42.593

100

0.435
  coiA Streptococcus pneumoniae Rx1

42.593

100

0.435

Multiple sequence alignment    



References

[1] Blandine David et al. (2017) Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2. Applied And Environmental Microbiology 83(16):e01074-17. [PMID: 28625996]