Detailed information    

experimental Experimentally validated

Overview

Name   coiA   Type   Machinery gene
Locus tag   SM12261_RS04415 Genome accession   NZ_CP028414
Coordinates   818654..819607 (+) Length   317 a.a.
NCBI ID   WP_000495920.1    Uniprot ID   A0A081SCJ1
Organism   Streptococcus mitis NCTC 12261     
Function   homologous recombination   
Homologous recombination

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 813654..824607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS04390 rpmG 814398..814547 (+) 150 WP_001809104.1 50S ribosomal protein L33 -
  SM12261_RS04395 (SM12261_0853) secG 814587..814820 (+) 234 WP_000282517.1 preprotein translocase subunit SecG -
  SM12261_RS04400 (SM12261_0854) rnr 814922..817276 (+) 2355 WP_000654876.1 ribonuclease R -
  SM12261_RS04405 (SM12261_0855) smpB 817239..817706 (+) 468 WP_001051750.1 SsrA-binding protein SmpB -
  SM12261_RS04410 (SM12261_0856) tehB 817721..818581 (+) 861 WP_000413782.1 SAM-dependent methyltransferase TehB -
  SM12261_RS04415 (SM12261_0857) coiA 818654..819607 (+) 954 WP_000495920.1 competence protein CoiA Machinery gene
  SM12261_RS04420 (SM12261_0858) pepF 819626..821428 (+) 1803 WP_000244089.1 oligoendopeptidase F Regulator
  SM12261_RS04425 (SM12261_0859) - 821430..822143 (+) 714 WP_004239376.1 O-methyltransferase -
  SM12261_RS04430 (SM12261_0860) prsA 822210..823148 (+) 939 WP_000727954.1 peptidylprolyl isomerase PrsA -
  SM12261_RS04435 (SM12261_0861) ftsW 823309..824532 (+) 1224 WP_000703370.1 cell division peptidoglycan polymerase FtsW -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 38161.88 Da        Isoelectric Point: 8.3929

>NTDB_id=495 SM12261_RS04415 WP_000495920.1 818654..819607(+) (coiA) [Streptococcus mitis NCTC 12261]
MFVARDSRGELVNVLEDKLEKQAYTCPACGGQLRLRQGPSVRIHFAHKTLKDCDFSSENESPEHLENKEVLYHWLKKEAE
VQLEYLLPELKQIADVFVNGNLALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGKKLWLKERLTRLQQGFLYFSQNMGF
YVWELDSEKQVLRLKYLIHQDLRGKLHYQIKEFPYGHGSLLEILRFPYKKQKISHFTVSQDKDICRYIRQQLYYQNPIWM
KEQAEAYKKGENLLTYGLKEWYPQIRPLVGNFCQIEQDLIRYYLYFQTYYQENPQNDWQMLYPPAFYQQYFLKNMVE

Nucleotide


Download         Length: 954 bp        

>NTDB_id=495 SM12261_RS04415 WP_000495920.1 818654..819607(+) (coiA) [Streptococcus mitis NCTC 12261]
ATGTTTGTTGCGAGAGATTCTAGGGGAGAATTGGTAAATGTGTTAGAGGATAAGCTTGAGAAGCAAGCATACACCTGCCC
AGCTTGTGGAGGTCAGCTCCGTTTGCGTCAAGGACCAAGTGTCCGGATCCATTTTGCCCACAAAACCTTAAAAGACTGTG
ATTTTTCCTCTGAAAATGAAAGTCCAGAACATCTGGAAAATAAGGAAGTCCTTTATCACTGGTTGAAAAAAGAGGCCGAG
GTGCAATTAGAATACCTGCTTCCAGAGCTTAAACAGATTGCGGATGTATTTGTAAATGGCAATCTAGCTTTAGAGGTTCA
ATGTAGTCCCTTGCCTCAAAAAGTTCTTAAAGAGCGTAGCGAGGGCTATCGTAGTCAGGGTTACCAAGTACTGTGGTTGC
TGGGAAAAAAACTGTGGCTCAAGGAGCGTTTGACTCGTCTACAACAAGGTTTTCTCTATTTCAGTCAAAACATGGGCTTT
TATGTTTGGGAATTAGACAGTGAAAAACAAGTTTTAAGACTCAAATACCTGATTCACCAAGATCTCCGAGGTAAACTCCA
TTATCAGATTAAGGAATTTCCCTATGGTCACGGTAGTTTACTGGAGATATTACGTTTTCCCTATAAGAAACAAAAAATAT
CTCATTTTACAGTTTCTCAGGACAAGGACATCTGTCGTTATATCCGGCAACAACTGTACTATCAAAATCCCATTTGGATG
AAAGAACAAGCAGAAGCCTATAAAAAGGGAGAAAATCTCCTGACTTATGGGCTAAAAGAATGGTATCCACAAATTCGACC
ACTAGTAGGTAATTTTTGCCAAATTGAGCAAGATTTGATTCGCTATTATCTGTATTTTCAAACCTATTATCAAGAAAATC
CTCAAAATGATTGGCAAATGCTCTATCCACCAGCCTTTTATCAGCAATATTTCTTAAAAAATATGGTAGAATAG

Domains

Predicted by InterproScan.

Competence protein CoiA-like family (CoiA_nuc)

(13-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A081SCJ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  coiA Streptococcus pneumoniae R6

89.274

100

0.893
  coiA Streptococcus pneumoniae D39

89.274

100

0.893
  coiA Streptococcus pneumoniae Rx1

89.274

100

0.893
  coiA Streptococcus pneumoniae TIGR4

89.274

100

0.893
  coiA Lactococcus lactis subsp. cremoris KW2

44.099

100

0.448

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]