Detailed information    

experimental Experimentally validated

Overview

Name   coiA   Type   Machinery gene
Locus tag   SPD_RS04655 Genome accession   NC_008533
Coordinates   879198..880151 (+) Length   317 a.a.
NCBI ID   WP_000495911.1    Uniprot ID   Q8DQ27
Organism   Streptococcus pneumoniae D39     
Function   homologous recombination   
Homologous recombination

Function

The coiA gene plays a role in the late stages of natural transformation, promoting the recombination of exogenous DNA.


Genomic Context

Location: 874198..885151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPD_RS04630 rpmG 874941..875090 (+) 150 WP_001810029.1 50S ribosomal protein L33 -
  SPD_RS04635 (SPD_0861) secG 875130..875363 (+) 234 WP_000282517.1 preprotein translocase subunit SecG -
  SPD_RS04640 (SPD_0862) rnr 875465..877819 (+) 2355 WP_000654897.1 ribonuclease R -
  SPD_RS04645 (SPD_0863) smpB 877782..878249 (+) 468 WP_001051750.1 SsrA-binding protein SmpB -
  SPD_RS04650 (SPD_0864) tehB 878265..879125 (+) 861 WP_000413762.1 SAM-dependent methyltransferase TehB -
  SPD_RS04655 (SPD_0865) coiA 879198..880151 (+) 954 WP_000495911.1 competence protein CoiA Machinery gene
  SPD_RS04660 (SPD_0866) pepF 880170..881972 (+) 1803 WP_000244122.1 oligoendopeptidase F Regulator
  SPD_RS04665 (SPD_0867) - 881974..882687 (+) 714 WP_000230399.1 O-methyltransferase -
  SPD_RS04670 (SPD_0868) prsA 882754..883695 (+) 942 WP_000727935.1 peptidylprolyl isomerase PrsA -
  SPD_RS04675 (SPD_0869) - 883814..884155 (-) 342 Protein_881 transposase -
  SPD_RS04685 - 884567..884662 (+) 96 Protein_882 IS630 family transposase -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 38143.79 Da        Isoelectric Point: 9.1135

>NTDB_id=224 SPD_RS04655 WP_000495911.1 879198..880151(+) (coiA) [Streptococcus pneumoniae D39]
MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTHFAHKSLKDCDFFFENESPEHLANKESLYHWLKKETK
VQLEYPLSELKQIADVFVNGNLALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
YVWELDKGKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTVSEDKDICRYIRQQLYYQNLFWM
KEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIEQDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE

Nucleotide


Download         Length: 954 bp        

>NTDB_id=224 SPD_RS04655 WP_000495911.1 879198..880151(+) (coiA) [Streptococcus pneumoniae D39]
ATGTTTGTTGCGAGAGATGCTAGGGGAGAATTGGTAAATGTGTTAGAGGATAAACTTGAGAAGCAAGCATACACCTGCCC
AGCTTGTGGAGGCCAGCTCCATTTGCGTCAAGGACCAAGTGTACGGACGCATTTTGCCCATAAATCTTTAAAAGACTGTG
ATTTTTTCTTTGAAAATGAAAGTCCAGAACACCTGGCCAATAAGGAATCCCTCTATCACTGGTTGAAAAAAGAGACAAAG
GTTCAATTAGAGTACCCGCTTTCAGAACTTAAACAGATTGCGGATGTATTTGTAAATGGCAATCTAGCTCTAGAAGTTCA
GTGTAGTCCCTTGCCTCAGAAAGTCCTTAAAGAGCGAAGTGAGGGCTATCGTAGTCAGGGTTACCAAGTACTGTGGTTGC
TGGGTCAAAAACTGTGGCTCAAGGAGCGTTTGACTCGTCTACAGCAAGGTTTTCTTTATTTCAGTCAAAACATGGGCTTT
TATGTTTGGGAATTAGACAAGGGAAAACAAGTTTTAAGACTCAAATACCTGATTTACCAGGATCTCCGCGGTAAACTCCA
TTATCAAATCAAGGAATTTTCCTATGGCCAAGGTAGTTTATTGGAAATATTGCGTCTTCCCTATAAGAAACAAAAAATAT
CTCATTTTACAGTTTCTGAGGACAAGGACATCTGTCGCTATATCCGGCAACAACTTTATTATCAAAATCTCTTTTGGATG
AAAGAACAAGCAGAAGCCTATCAAAAGGGAGAAAATATCCTGACTTATGGACTGAAAGAATGGTATCCACAAATTCGACC
AATAGTGGGCAAATTTTTCCAGATTGAACAAGACTTGACTAGCTATTATCAGCACTTTTATACCTATTACCAAAAAAATC
CTCAAAATGATTGGCAAAAGCTTTATCCACCAGCCTTTTATCAGCAATATTTCTTGAAAAATATGGTAGAATAG

Domains

Predicted by InterproScan.

Competence protein CoiA-like family (CoiA_nuc)

(13-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DQ27

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  coiA Streptococcus pneumoniae Rx1

100

100

1
  coiA Streptococcus pneumoniae R6

100

100

1
  coiA Streptococcus pneumoniae TIGR4

99.369

100

0.994
  coiA Streptococcus mitis NCTC 12261

89.274

100

0.893
  coiA Lactococcus lactis subsp. cremoris KW2

42.593

100

0.435

Multiple sequence alignment    



References

[1] Bhushan V Desai et al. (2007) Transformation in Streptococcus pneumoniae: formation of eclipse complex in a coiA mutant implicates CoiA in genetic recombination. Molecular Microbiology 63(4):1107-17. [PMID: 17233830]
[2] Bhushan V Desai et al. (2006) An unstable competence-induced protein, CoiA, promotes processing of donor DNA after uptake during genetic transformation in Streptococcus pneumoniae. Journal of Bacteriology 188(14):5177-86. [PMID: 16816189]
[3] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]