Detailed information    

experimental Experimentally validated

Overview

Name   coiA   Type   Machinery gene
Locus tag   SP_RS04850 Genome accession   NC_003028
Coordinates   923559..924512 (+) Length   317 a.a.
NCBI ID   WP_000495909.1    Uniprot ID   A0A0H2UPP6
Organism   Streptococcus pneumoniae TIGR4     
Function   homologous recombination   
Homologous recombination

Function

The coiA gene plays a role in the late stages of natural transformation, promoting the recombination of exogenous DNA.


Genomic Context

Location: 918559..929512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS04825 (SP_0973) rpmG 919302..919451 (+) 150 WP_001809104.1 50S ribosomal protein L33 -
  SP_RS04830 (SP_0974) secG 919491..919724 (+) 234 WP_000282517.1 preprotein translocase subunit SecG -
  SP_RS04835 (SP_0975) rnr 919826..922180 (+) 2355 WP_000654907.1 ribonuclease R -
  SP_RS04840 (SP_0976) smpB 922143..922610 (+) 468 WP_001051747.1 SsrA-binding protein SmpB -
  SP_RS04845 (SP_0977) tehB 922626..923486 (+) 861 WP_000413762.1 SAM-dependent methyltransferase TehB -
  SP_RS04850 (SP_0978) coiA 923559..924512 (+) 954 WP_000495909.1 competence protein CoiA Machinery gene
  SP_RS04855 (SP_0979) pepF 924531..926333 (+) 1803 WP_000244116.1 oligoendopeptidase F Regulator
  SP_RS04860 (SP_0980) - 926335..927048 (+) 714 WP_000230399.1 O-methyltransferase -
  SP_RS04865 (SP_0981) prsA 927115..928056 (+) 942 WP_000727952.1 peptidylprolyl isomerase PrsA -
  SP_RS04870 (SP_0982) - 928174..928515 (-) 342 Protein_926 transposase -
  SP_RS12765 - 928928..929023 (+) 96 Protein_927 IS630 family transposase -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 38243.86 Da        Isoelectric Point: 8.9865

>NTDB_id=155 SP_RS04850 WP_000495909.1 923559..924512(+) (coiA) [Streptococcus pneumoniae TIGR4]
MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTHFAHKSLKDCDFFFENESPEHLANKESLYHWLKKETK
VQLEYPLSELKQIADVFVNGNLALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTVSEDKDICRYIRQQLYYQNLFWM
KEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIEQDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE

Nucleotide


Download         Length: 954 bp        

>NTDB_id=155 SP_RS04850 WP_000495909.1 923559..924512(+) (coiA) [Streptococcus pneumoniae TIGR4]
ATGTTTGTTGCGAGAGATGCTAGGGGAGAATTGGTAAATGTGTTAGAGGATAAACTTGAGAAGCAAGCATACACCTGCCC
AGCTTGTGGAGGCCAGCTCCATTTGCGTCAAGGACCAAGTGTACGGACGCATTTTGCCCATAAATCCTTAAAAGACTGTG
ATTTTTTCTTTGAAAATGAAAGTCCAGAACACCTGGCCAATAAGGAATCCCTCTATCACTGGTTGAAAAAAGAGACAAAG
GTTCAATTAGAGTACCCGCTTTCAGAACTTAAACAGATTGCGGATGTATTTGTAAATGGCAATCTAGCTCTAGAAGTTCA
GTGTAGTCCCTTGCCTCAGAAAGTCCTTAAAGAGCGAAGTGAGGGCTATCGTAGTCAGGGTTACCAAGTACTGTGGTTGC
TGGGTCAAAAACTGTGGCTCAAGGAGCGTTTGACTCGTCTACAGCAAGGTTTTCTTTATTTCAGTCAAAACATGGGCTTT
TATGTTTGGGAATTAGACAAGGAAAAACAAGTTTTAAGACTCAAATACCTGATTTACCAGGATCTCCGCGGTAAACTCCA
TTATCAAATCAAGGAATTTTCCTATGGTCAAGGTAGTTTATTGGAAATATTGCGTCTTCCCTATAAGAGACAAAAAATAT
CTCATTTTACAGTTTCTGAGGACAAGGACATCTGTCGCTATATCCGGCAACAACTTTATTATCAAAATCTCTTTTGGATG
AAAGAACAAGCAGAAGCCTATCAAAAGGGAGAAAATATCCTGACTTATGGACTGAAAGAATGGTATCCACAAATTCGACC
AATAGTGGGCAAATTTTTCCAGATTGAACAAGACTTGACTAGCTATTATCAGCACTTTTATACCTATTACCAAAAAAATC
CTCAAAATGATTGGCAAAAGCTTTATCCACCAGCCTTTTATCAGCAATATTTCTTGAAAAATATGGTAGAATAG

Domains

Predicted by InterproScan.

Competence protein CoiA-like family (CoiA_nuc)

(13-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2UPP6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  coiA Streptococcus pneumoniae D39

99.369

100

0.994
  coiA Streptococcus pneumoniae R6

99.369

100

0.994
  coiA Streptococcus pneumoniae Rx1

99.369

100

0.994
  coiA Streptococcus mitis NCTC 12261

89.274

100

0.893
  coiA Lactococcus lactis subsp. cremoris KW2

42.593

100

0.435

Multiple sequence alignment    



References

[1] Bhushan V Desai et al. (2007) Transformation in Streptococcus pneumoniae: formation of eclipse complex in a coiA mutant implicates CoiA in genetic recombination. Molecular Microbiology 63(4):1107-17. [PMID: 17233830]
[2] Bhushan V Desai et al. (2006) An unstable competence-induced protein, CoiA, promotes processing of donor DNA after uptake during genetic transformation in Streptococcus pneumoniae. Journal of Bacteriology 188(14):5177-86. [PMID: 16816189]
[3] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]