Detailed information    

experimental Experimentally validated

Overview

Name   comX   Type   Regulator
Locus tag   V4T04_RS13125 Genome accession   NZ_JAZICS010000016
Coordinates   33531..34022 (+) Length   163 a.a.
NCBI ID   WP_349652201.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain DGCC12653     
Function   activation of late competence genes   
Competence regulation

Function

Natural transformation in this species requires the activation of the master regulator ComX.


Genomic Context

Location: 28531..39022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4T04_RS13110 rpsB 29422..30189 (+) 768 WP_011836067.1 30S ribosomal protein S2 -
  V4T04_RS13115 tsf 30311..31339 (+) 1029 WP_015427183.1 translation elongation factor Ts -
  V4T04_RS13120 ezrA 31544..33274 (+) 1731 WP_003132670.1 septation ring formation regulator EzrA -
  V4T04_RS13125 comX 33531..34022 (+) 492 WP_349652201.1 sigma-70 family RNA polymerase sigma factor Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  clpP comX negative effect
  mecA comX negative effect
  ccpA comX negative effect
  codY comX negative effect
  covR comX negative effect
  covS comX negative effect
  clpC comX negative effect

Sequence

Protein


Download         Length: 163 a.a.        Molecular weight: 19817.71 Da        Isoelectric Point: 5.6118

>NTDB_id=608 V4T04_RS13125 WP_349652201.1 33531..34022(+) (comX) [Lactococcus lactis subsp. lactis strain DGCC12653]
MTYYLEEEDFENLFLEMKPIVMKLMKQIRIRTWKIEDYLQEGMIILHLLLEEQSDGQKLHTKFKVKYHQRLIDELRRSYA
KKRSHDHFIGLDVYECSDWINSGDTSPDNEVVFNHLLAEVYEGLSAHYQDLLLRQMRGEELTRMQRYRLREKIKAILFSE
DEE

Nucleotide


Download         Length: 492 bp        

>NTDB_id=608 V4T04_RS13125 WP_349652201.1 33531..34022(+) (comX) [Lactococcus lactis subsp. lactis strain DGCC12653]
ATGACATATTACTTGGAAGAAGAGGATTTTGAAAATCTGTTTTTAGAAATGAAACCTATAGTTATGAAATTAATGAAACA
AATTCGCATTAGAACATGGAAAATAGAGGATTATCTTCAAGAGGGGATGATTATTTTACATCTTCTATTAGAAGAGCAGA
GCGATGGTCAAAAGCTGCATACAAAATTTAAGGTAAAGTATCATCAAAGATTAATAGATGAATTAAGACGAAGTTATGCA
AAGAAACGAAGCCATGACCATTTTATAGGTTTAGATGTTTATGAATGCTCAGACTGGATAAATTCAGGTGATACTAGTCC
AGATAATGAAGTGGTCTTCAATCATTTGCTGGCAGAAGTATATGAAGGTTTGAGCGCACATTATCAAGACTTACTACTTC
GACAAATGCGAGGAGAAGAACTAACTCGCATGCAACGGTATCGCCTTCGTGAAAAAATAAAGGCCATCTTATTTTCAGAA
GACGAAGAGTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comX Lactococcus lactis subsp. cremoris KW2

75.46

100

0.755

Multiple sequence alignment    



References

[1] Frédéric Toussaint et al. (2024) Unveiling the regulatory network controlling natural transformation in lactococci. PLoS Genetics 20(7):e1011340. [PMID: 38950059]