Detailed information NTG0000627

Experimentally validated

Overview

• Name: comE
• Type: Regulator
• Locus tag: -
• Genome accession: AF498313
• Coordinates: 1453..2205 (+)
• Length: 250 a.a.
• NCBI ID: AAM18091.1
• Uniprot ID: -
• Organism: Streptococcus infantis strain Atu-4
• Function: activate transcription of early competence genes Competence regulation

Function

A natural isolate of a mitis group streptococcus (Atu-4) is competent for genetic transformation even though it has lost the gene encoding the competence pheromone. Competence in the Atu-4 strain is lost if the gene encoding the response regulator ComE is disrupted, demonstrating that this component of the quorum-sensing apparatus is still needed for competence development.

Genomic Context

Location: 1..7205

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Locus_0	comD	133..1452 (+)	1320	AAM18090.1	histidine kinase ComD	Regulator
Locus_1	comE	1453..2205 (+)	753	AAM18091.1	response regulator ComE	Regulator

Sequence

Protein

• Length: 250 a.a.
• Molecular weight: 29855.27 Da
• Isoelectric Point: 8.0329

>NTDB_id=627 AAM18091.1 1453..2205(+) (comE) [Streptococcus infantis strain Atu-4]
MKVLILEDVIQHQVRLERTLDEISKELNIPISYKTTGKEREFKEYIENDEVNQLYFLDIDIHGVEKKGFEVAQFIRRHNP
YAIIVFITSRSEFATLTYKYQVSALDFVDKDINDELFKKRIEQSILYTKSMLLENKDVVDYFDYNYKGNDLKIPYHDILY
IETTGVSHKLRIIGKNFAKEFYGTMADIQEKDKDTQRFYSAHKSFLVNVGNIREIDRKNLEVVFYEDHRCPITRLKVRKL
RDILEKKSKK*

Nucleotide

• Length: 753 bp

>NTDB_id=627 AAM18091.1 1453..2205(+) (comE) [Streptococcus infantis strain Atu-4]
ATGAAAGTATTAATTTTAGAAGATGTTATTCAACATCAAGTCAGATTAGAAAGAACCTTGGATGAAATTTCAAAGGAATT
GAATATTCCTATTTCTTACAAAACAACAGGTAAAGAACGAGAATTTAAGGAATATATCGAAAATGATGAAGTAAACCAGC
TTTATTTTCTAGATATTGATATTCATGGCGTTGAGAAAAAAGGATTTGAGGTAGCACAGTTTATTCGTCGCCACAATCCC
TATGCAATTATCGTATTTATCACTAGTAGATCAGAATTTGCTACCTTAACTTACAAATATCAAGTATCAGCCTTGGATTT
TGTTGATAAAGATATCAATGATGAATTATTTAAAAAACGTATCGAACAGAGTATCTTATATACCAAAAGTATGCTACTTG
AAAATAAAGATGTTGTGGATTATTTTGACTATAATTACAAGGGAAATGATTTGAAAATTCCCTACCATGATATTTTATAT
ATTGAAACAACAGGAGTATCTCATAAGTTAAGGATTATTGGTAAGAATTTTGCCAAAGAATTTTATGGTACTATGGCGGA
TATTCAAGAAAAAGATAAAGATACTCAAAGATTTTATTCTGCTCATAAATCATTTCTTGTCAATGTAGGAAATATTAGGG
AAATTGATCGAAAAAATTTAGAAGTGGTTTTTTATGAGGATCATCGTTGTCCAATTACCCGTCTAAAGGTACGGAAATTA
AGAGATATTCTGGAGAAAAAATCTAAAAAGTGA

Domains

Predicted by InterproScan.

• LytTr DNA-binding domain (LytTR) (151-244)

• Response regulator receiver domain (Response_reg) (32-121)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Streptococcus mitis NCTC 12261	92.8	100	0.928
comE	Streptococcus mitis SK321	92	100	0.92
comE	Streptococcus pneumoniae Rx1	91.2	100	0.912
comE	Streptococcus pneumoniae TIGR4	91.2	100	0.912
comE	Streptococcus pneumoniae R6	91.2	100	0.912
comE	Streptococcus pneumoniae D39	91.2	100	0.912
comE/comE2	Streptococcus gordonii strain NCTC7865	64	99.602	0.637
comE/comE1	Streptococcus gordonii str. Challis substr. CH1	64	99.602	0.637
comE/blpR	Streptococcus mutans UA159	41.7	98.8	0.412

References

• [1]: Ola Ween et al. (2002) Competence without a competence pheromone in a natural isolate of Streptococcus infantis. Journal of Bacteriology 184(13):3426-32. [PMID: 12057935]