Detailed information    

experimental Experimentally validated

Overview

Name   comD/comD3   Type   Regulator
Locus tag   H702_RS02380 Genome accession   NZ_AUZH01000012
Coordinates   10877..12253 (+) Length   458 a.a.
NCBI ID   WP_039696235.1    Uniprot ID   A0A091BV28
Organism   Streptococcus equinus JB1     
Function   phosphorylation of ComE   
Competence regulation

Function

The transformation frequency was decreased by the disruption of comCD. Addition of recombinant ComC to S. bovis cultures increased the growth rate and transformation frequency.


Genomic Context

Location: 5877..17253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H702_RS10470 comD/comD1 7219..7941 (+) 723 WP_236726575.1 sensor histidine kinase Regulator
  H702_RS10235 (H702_02440) comC/comC1 7951..8094 (-) 144 WP_160172983.1 hypothetical protein Regulator
  H702_RS02355 (H702_02445) - 8358..8507 (+) 150 WP_039696228.1 hypothetical protein -
  H702_RS02360 (H702_02450) - 8520..8825 (+) 306 WP_039696229.1 bacteriocin immunity protein -
  H702_RS02365 (H702_02455) - 8853..9146 (+) 294 WP_039696231.1 bacteriocin immunity protein -
  H702_RS02375 (H702_02465) comD/comD2 9933..10880 (+) 948 WP_160172984.1 sensor histidine kinase Regulator
  H702_RS02380 (H702_02470) comD/comD3 10877..12253 (+) 1377 WP_039696235.1 sensor histidine kinase Regulator
  H702_RS02385 (H702_02475) comE/comE2 12914..13648 (+) 735 WP_052071091.1 LytR/AlgR family response regulator transcription factor Regulator
  H702_RS02395 (H702_02485) rsgA 13966..14838 (+) 873 WP_039696236.1 ribosome small subunit-dependent GTPase A -
  H702_RS02400 (H702_02490) rpe 14845..15507 (+) 663 WP_006531895.1 ribulose-phosphate 3-epimerase -
  H702_RS02405 (H702_02495) - 15500..16132 (+) 633 WP_039696237.1 thiamine diphosphokinase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 52937.23 Da        Isoelectric Point: 6.6140

>NTDB_id=544 H702_RS02380 WP_039696235.1 10877..12253(+) (comD/comD3) [Streptococcus equinus JB1]
MIEIVVFIITMLLNFVGFVIVGILFQQITKVKLTFLEWVLIIIFLQAVSFFMNPQKYSMVALVLMKDISVIFCLLSVSFH
HNPKIGIRYHIFYALYPINLYMILHEVYCYIIFAIFSIQLTSLTMTSPSVGKMIGMITAVIIPPIFYYLISKWLNIDISN
VRKTLKNYLSARALRFVNVLLLLFYPGMECFGAIAPFEKMSMNRNIYRAVIFIYFILLFIFLFYLNFRHGQEQKNELLRN
QEMELAALENYSKHVESLYQEVRSFRHDYANILMSLKVGIDQGNLDDIKKIYDEITADSTKLVKNNKFDLTRLANITDSG
VKSLMSAKFLQAENQGITISLEVAEEMGTPKIPLISYIRILSILFDNAIEAALESAEPRISVANFYQDDEFVFMIENSTK
EKSVDLGKIFERGYSTKGDNRGLGLATLMDFQDDYENLSVETRSLDYKFTQVVRIYEA

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=544 H702_RS02380 WP_039696235.1 10877..12253(+) (comD/comD3) [Streptococcus equinus JB1]
ATGATTGAGATTGTTGTATTTATTATTACAATGCTTTTGAATTTTGTGGGATTCGTTATAGTAGGGATTCTATTTCAACA
AATAACAAAGGTTAAATTAACGTTTTTAGAATGGGTATTGATAATCATATTTCTTCAAGCAGTTTCTTTTTTTATGAATC
CTCAGAAATATTCAATGGTTGCTTTAGTACTAATGAAAGATATTTCAGTGATTTTTTGTTTACTATCAGTTTCTTTTCAT
CATAATCCCAAAATAGGAATAAGATATCACATTTTTTATGCCTTGTATCCAATTAATCTTTATATGATTCTACACGAAGT
TTATTGTTATATTATATTTGCAATTTTTTCAATACAATTGACCTCACTTACAATGACTTCACCATCAGTAGGAAAAATGA
TTGGTATGATAACTGCAGTTATTATTCCACCAATTTTTTACTATCTAATATCAAAGTGGCTTAACATTGATATTTCAAAT
GTCAGAAAAACGCTCAAGAATTATCTTAGTGCAAGAGCGCTTAGATTTGTTAATGTATTATTACTACTTTTCTATCCAGG
AATGGAATGTTTTGGTGCCATAGCCCCTTTTGAGAAAATGTCGATGAATAGAAATATTTATAGGGCTGTTATTTTTATCT
ACTTCATTCTTCTTTTTATTTTCCTCTTTTATCTCAATTTCCGTCATGGACAAGAGCAGAAAAATGAATTGCTTCGCAAT
CAGGAGATGGAATTGGCGGCCTTGGAAAATTATAGCAAACACGTGGAAAGCCTTTATCAAGAAGTGCGCAGTTTCAGGCA
TGACTACGCTAATATTCTGATGAGTTTAAAGGTTGGAATTGATCAAGGCAATCTTGATGACATCAAGAAAATTTACGATG
AAATCACAGCCGATTCAACGAAACTAGTCAAGAATAATAAATTTGACTTGACTCGTTTGGCTAACATCACAGACAGCGGC
GTCAAAAGTCTGATGTCTGCTAAATTTTTGCAGGCGGAAAATCAGGGTATCACGATTAGTTTGGAAGTTGCTGAAGAAAT
GGGAACTCCAAAGATTCCATTGATTTCCTATATTCGAATTTTGTCAATTTTGTTTGACAATGCCATAGAAGCCGCTTTGG
AGTCTGCGGAGCCAAGAATCTCAGTCGCCAATTTCTACCAAGATGATGAGTTTGTTTTTATGATTGAAAATTCAACTAAG
GAGAAATCAGTGGATTTAGGCAAGATTTTTGAGCGTGGCTACTCAACCAAAGGAGATAATCGCGGCCTCGGCCTAGCGAC
GCTAATGGATTTTCAAGATGACTACGAAAATTTATCGGTTGAAACCCGCAGTTTAGATTATAAATTTACACAAGTAGTGA
GAATTTACGAGGCGTAA

Domains

Predicted by InterproScan.

GHKL domain (HATPase_c_5)

(358-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A091BV28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comD/comD2 Streptococcus equinus JB1

79.167

99.048

0.784
  comD/comD1 Streptococcus equinus JB1

50.216

96.25

0.483

Multiple sequence alignment    



References

[1] Narito Asanuma et al. (2010) Involvement of two-component signal transduction system, ComDE, in the regulation of growth and genetic transformation, in the ruminal bacterium Streptococcus bovis. Anaerobe 16(4):405-11. [PMID: 20478389]