Detailed information    

experimental Experimentally validated

Overview

Name   comR   Type   Regulator
Locus tag   SSU05_0053 Genome accession   CP000407
Coordinates   55400..56314 (+) Length   304 a.a.
NCBI ID   ABP89025.1    Uniprot ID   -
Organism   Streptococcus suis 05ZYH33     
Function   activate transcription of comX   
Competence regulation

Function

We developed a 'test-bed' assay to measure the activity of different ComR proteins in response to cognate and heterologous XIP peptides in vivo, revealing distinct ComR classes of strict, intermediate, and promiscuous specificity among species. We then solved an X-ray crystal structure of ComR from S. suis to further understand the interaction with XIP and to search for structural features in ComR proteins that may explain XIP recognition.


Genomic Context

Location: 50400..61314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SSU05_0047 - 50501..50983 (+) 483 ABP89019.1 ABC-type multidrug transport system, ATPase and permease component -
  SSU05_0048 - 50991..52214 (+) 1224 ABP89020.1 ABC-type multidrug transport system, ATPase and permease component -
  SSU05_0049 - 52316..52489 (+) 174 ABP89021.1 hypothetical protein -
  SSU05_0050 - 52784..53785 (+) 1002 ABP89022.1 Holliday junction resolvasome, helicase subunit -
  SSU05_0051 - 53785..54531 (+) 747 ABP89023.1 hypothetical protein -
  SSU05_0052 - 54533..55168 (+) 636 ABP89024.1 Predicted phosphatase -
  SSU05_0053 comR 55400..56314 (+) 915 ABP89025.1 Predicted transcriptional regulator Regulator
  SSU05_0054 - 56719..57882 (+) 1164 ABP89026.1 Transposase and inactivated derivative -
  SSU05_0055 - 58420..59358 (-) 939 ABP89027.1 Transposase and inactivated derivative -
  SSU05_0056 - 59492..60718 (+) 1227 ABP89028.1 Folylpolyglutamate synthase -
  SSU05_0057 - 60743..60856 (+) 114 ABP89029.1 hypothetical protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comR comX/sigX positive effect

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 36098.13 Da        Isoelectric Point: 4.6131

>NTDB_id=465 SSU05_0053 ABP89025.1 55400..56314(+) (comR) [Streptococcus suis 05ZYH33]
MEVWFMNDKEFGQRVRQLRESASMTREQFCDDELELSVRQLTRIEAGASKPTFSKIQYIATRLGMGLYELMPDYVSLPER
YSKLKFDVLRTPTYGNEDLAEKRDAMMTEIYDDYYDELPEEEKIAIDAIQSRIDTLESGTAGFGKEILEDYFEQIFRKRK
YELNDLLIVRLHLEYVRLSSCDSEIFRQFLKIIEHLHEQINIINSNDLFVLRDTLLSCVNILGSKKYYEPIPKIFDSVDK
IIQSTQDFQKKPIVSVLKWKYALFVDKDRDEAEKHYLDAVLFAKLIENRELEQKIEEDWRVDNQ

Nucleotide


Download         Length: 915 bp        

>NTDB_id=465 SSU05_0053 ABP89025.1 55400..56314(+) (comR) [Streptococcus suis 05ZYH33]
TTGGAGGTGTGGTTTATGAACGATAAGGAATTTGGACAGCGTGTACGTCAATTACGAGAATCTGCTAGTATGACACGTGA
ACAGTTTTGTGACGATGAACTGGAACTCTCTGTGCGCCAATTAACTCGTATTGAAGCAGGTGCTTCCAAGCCGACTTTTT
CAAAGATTCAGTATATTGCAACTCGTTTAGGTATGGGACTTTACGAGCTTATGCCAGATTATGTATCTTTACCCGAAAGA
TATTCCAAGCTGAAGTTTGATGTGCTTCGCACCCCAACTTATGGTAATGAAGATTTGGCGGAAAAGCGAGATGCCATGAT
GACAGAAATCTATGACGATTATTATGATGAATTGCCTGAGGAGGAGAAGATAGCAATAGATGCGATTCAATCACGAATTG
ATACTTTAGAGTCAGGTACAGCAGGCTTTGGAAAAGAGATACTGGAAGACTACTTTGAACAAATTTTTCGCAAACGAAAG
TATGAATTGAATGATTTGTTGATTGTTAGGCTCCATCTTGAATATGTTAGGTTATCTAGCTGTGATTCAGAAATATTTAG
ACAGTTTTTGAAAATTATAGAGCATTTACATGAGCAAATCAATATCATCAACTCAAATGATTTATTTGTTTTACGAGACA
CGCTATTATCTTGTGTAAATATATTAGGAAGTAAAAAATATTACGAACCAATACCAAAGATATTTGACAGTGTAGATAAG
ATTATACAGTCGACACAAGATTTTCAGAAAAAGCCCATTGTTAGTGTATTAAAATGGAAATATGCACTTTTTGTGGATAA
GGATCGGGATGAGGCAGAAAAGCATTATCTAGATGCGGTGCTATTTGCAAAATTGATAGAAAATAGAGAGTTAGAACAGA
AGATTGAAGAAGATTGGAGAGTTGACAATCAATAA

Domains

Predicted by InterproScan.

ComR tetratricopeptide (ComR_TPR)

(78-299)

Helix-turn-helix domain (HTH_31)

(10-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comR Streptococcus suis P1/7

100

100

1
  comR Streptococcus suis D9

62.126

99.013

0.615
  comR Streptococcus mutans UA159

43.813

98.355

0.431
  comR Streptococcus infantarius subsp. infantarius ATCC BAA-102

37.153

98.63

0.366
  comR Streptococcus pyogenes MGAS315

37.374

98.02

0.366

Multiple sequence alignment    



References

[1] Erin Shanker et al. (2016) Pheromone Recognition and Selectivity by ComR Proteins among Streptococcus Species. PLoS Pathogens 12(12):e1005979. [PMID: 27907154]