Detailed information NTG0000458

Experimentally validated

Overview

• Name: comR
• Type: Regulator
• Locus tag: SSUD9_RS00355
• Genome accession: NC_017620
• Coordinates: 59566..60483 (+)
• Length: 305 a.a.
• NCBI ID: WP_002935296.1
• Uniprot ID: -
• Organism: Streptococcus suis D9
• Function: activate transcription of comX Competence regulation

Function

Induction of competence was dependent on ComX, a sigma factor that controls the streptococcal late competence regulon, extracellular addition of a comX-inducing peptide (XIP), and ComR, a regulator of comX. XIP was identified as an N-terminally truncated variant of ComS.

Genomic Context

Location: 54566..65483

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SSUD9_RS00335 (SSUD9_0051)	-	54650..56362 (+)	1713	WP_002935303.1	ABC transporter ATP-binding protein	-
SSUD9_RS11460 (SSUD9_0052)	-	56464..56637 (+)	174	WP_014636741.1	hypothetical protein	-
SSUD9_RS00340 (SSUD9_0053)	ruvB	56932..57933 (+)	1002	WP_002935300.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
SSUD9_RS00345 (SSUD9_0054)	-	57933..58679 (+)	747	WP_002935299.1	GNAT family N-acetyltransferase	-
SSUD9_RS00350 (SSUD9_0055)	-	58681..59319 (+)	639	WP_002935298.1	HAD-IA family hydrolase	-
SSUD9_RS00355 (SSUD9_0056)	comR	59566..60483 (+)	918	WP_002935296.1	helix-turn-helix domain-containing protein	Regulator
SSUD9_RS00360 (SSUD9_0058)	-	60892..62094 (-)	1203	WP_002935295.1	IS110-like element ISSsu7 family transposase	-
SSUD9_RS00365 (SSUD9_0059)	-	62371..63612 (+)	1242	WP_013730059.1	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	-
SSUD9_RS00375 (SSUD9_0061)	-	63781..65304 (+)	1524	WP_004194221.1	Heme/copper-type cytochrome/quinol oxidase subunit 1	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comR	comX/sigX	positive effect
comX/sigX	late competence genes	positive effect
comX/sigX	late competence genes	positive effect
comS	comX/sigX	positive effect

Sequence

Protein

• Length: 305 a.a.
• Molecular weight: 36252.32 Da
• Isoelectric Point: 4.6744

>NTDB_id=458 SSUD9_RS00355 WP_002935296.1 59566..60483(+) (comR) [Streptococcus suis D9]
MNDKEFGQRVRQLRESASMTREQFCDDELELSVRQLTRIEAGASKPTFSKIQYIATRLGMGLYELMPDYVSLPERYSKLK
FDVLRTPTYGNEDLAEKRDAMMTEIYDDYYDELPEEEKIVVDTLCSLFDVLDTDSQEYGKEILDDYLHQSYHRSKLSIND
LMILRLFIEHCQLEDLSVGTSNYALFTDLIEKLPQSIYDVHSESLFIVRDLFLAIVRILFSKELYNHIPVYIEKIENIME
LSQDFQKKPILNLVKWKYELKVQHNHEIAERYYNEAITFASLLNQFHLKEKLQMEWEKDTQSLKR

Nucleotide

• Length: 918 bp

>NTDB_id=458 SSUD9_RS00355 WP_002935296.1 59566..60483(+) (comR) [Streptococcus suis D9]
ATGAACGATAAGGAGTTTGGACAGCGTGTGCGTCAATTACGAGAATCTGCTAGTATGACACGTGAACAGTTTTGTGACGA
TGAACTGGAACTCTCTGTGCGCCAATTAACTCGTATTGAAGCAGGTGCTTCCAAGCCGACTTTTTCAAAGATTCAGTATA
TTGCAACTCGTTTAGGTATGGGACTTTACGAGCTTATGCCAGATTATGTATCTTTACCCGAAAGATATTCCAAGCTGAAG
TTTGATGTGCTTCGCACCCCAACTTATGGTAATGAAGATTTGGCGGAAAAGCGAGATGCCATGATGACAGAAATCTATGA
CGATTATTATGATGAATTGCCTGAGGAGGAGAAAATAGTTGTAGACACCCTATGTTCCCTATTTGATGTATTGGATACAG
ATAGCCAGGAATACGGTAAAGAAATTCTTGATGATTATTTACATCAAAGCTATCACCGTTCCAAACTATCTATAAACGAT
TTAATGATTTTGCGATTATTTATTGAACATTGTCAGTTGGAGGATTTATCTGTTGGAACTTCCAATTATGCTTTATTTAC
AGATTTGATAGAGAAATTGCCTCAATCAATCTATGATGTACATTCGGAATCCTTATTCATTGTCCGCGATTTATTTCTAG
CTATTGTTCGTATTTTATTTTCGAAGGAGTTGTATAATCATATTCCAGTCTATATAGAAAAAATAGAAAACATCATGGAA
TTATCACAAGATTTTCAGAAGAAGCCAATTCTAAATCTTGTGAAATGGAAATATGAATTAAAGGTACAACATAATCATGA
AATTGCTGAGAGGTACTATAATGAAGCGATTACATTTGCCAGCCTACTAAATCAATTTCACTTGAAAGAAAAACTGCAAA
TGGAATGGGAAAAAGATACGCAGAGTCTAAAAAGATGA

Domains

Predicted by InterProScan.

• Helix-turn-helix domain (HTH_31) (5-60)

• ComR tetratricopeptide (ComR_TPR) (73-296)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comR	Streptococcus suis P1/7	62.126	100	0.625
comR	Streptococcus suis 05ZYH33	62.126	99.013	0.615
comR	Streptococcus infantarius subsp. infantarius ATCC BAA-102	36.86	100	0.37
comR	Streptococcus mutans UA159	37	98.684	0.365

References

• [1]: Edoardo Zaccaria et al. (2014) Control of competence for DNA transformation in streptococcus suis by genetically transferable pherotypes. PloS One 9(6):e99394. [PMID: 24968201]