Detailed information    

experimental Experimentally validated

Overview

Name   comX/sigX/comX2/sigX2   Type   Regulator
Locus tag   SPYM3_RS08515 Genome accession   NC_004070
Coordinates   1640888..1641439 (-) Length   183 a.a.
NCBI ID   WP_011106595.1    Uniprot ID   A0A5S4TPS4
Organism   Streptococcus pyogenes MGAS315     
Function   activate transcription of late competence genes   
Competence regulation

Function

SigX is functional and able to activate sigX-dependent gene expression within the competence regulon.


Genomic Context

Location: 1635888..1646439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYM3_RS08490 (SpyM3_1635) - 1637254..1638099 (+) 846 WP_002982833.1 mechanosensitive ion channel family protein -
  SPYM3_RS08495 (SpyM3_1636) - 1638164..1638724 (-) 561 WP_002995157.1 TIGR01440 family protein -
  SPYM3_RS08500 (SpyM3_1637) - 1638738..1639208 (-) 471 WP_011106596.1 ECF transporter S component -
  SPYM3_RS08505 (SpyM3_1638) - 1639198..1639962 (-) 765 WP_002982821.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  SPYM3_RS08510 (SpyM3_1639) truA 1639952..1640701 (-) 750 WP_002995162.1 tRNA pseudouridine(38-40) synthase TruA -
  SPYM3_RS08515 (SpyM3_1640) comX/sigX/comX2/sigX2 1640888..1641439 (-) 552 WP_011106595.1 sigma-70 family RNA polymerase sigma factor Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comR comX/sigX/comX1/sigX1 positive effect
  comS comX/sigX/comX1/sigX1 positive effect
  clpP comX/sigX/comX1/sigX1 negative effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comR comX/sigX/comX2/sigX2 positive effect
  comS comX/sigX/comX2/sigX2 positive effect
  clpP comX/sigX/comX2/sigX2 negative effect
  prx comR negative effect

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 22309.13 Da        Isoelectric Point: 10.0375

>NTDB_id=442 SPYM3_RS08515 WP_011106595.1 1640888..1641439(-) (comX/sigX/comX2/sigX2) [Streptococcus pyogenes MGAS315]
MSCCFFVVLFILGIVKKKRRLKMSIETRAVFEKVKPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERL
YRYFKTKFSSYLKDLLRRQESQKRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQERMQFQALIRGE
RFKGRRALLRKISPYFKEFAQQL

Nucleotide


Download         Length: 552 bp        

>NTDB_id=442 SPYM3_RS08515 WP_011106595.1 1640888..1641439(-) (comX/sigX/comX2/sigX2) [Streptococcus pyogenes MGAS315]
ATGTCCTGTTGTTTTTTTGTGGTTCTCTTTATACTAGGGATAGTTAAAAAGAAAAGGAGACTCAAAATGTCGATAGAGAC
AAGAGCAGTGTTTGAAAAAGTTAAGCCCATTATTTTGAAATTAAAGCGACACTATTATATTCAATTGTGGGATAGAGATG
ACTGGTTACAAGAAGGACATATTATTTTATTACAGTTACTAGAAAGGTACCCAGAGTTAATTGAGGAAGAAGAGCGTTTA
TATCGCTATTTTAAAACAAAATTTTCATCTTATTTGAAAGATTTATTACGCCGTCAAGAAAGTCAAAAGCGTCAGTTCCA
TAAGTTAGCATATGAAGAGATAGGGGAGGTTGCACATGCCATTCCATCGAGAGGGTTATGGCTAGACGACTATGTGGCTT
ATCAAGAGGTAATAGCTAGCTTAGAGAACCAATTAAATTCGCAAGAGCGTATGCAGTTTCAAGCACTTATCAGGGGCGAA
CGTTTCAAGGGAAGACGTGCTCTACTTAGGAAGATCAGTCCCTATTTTAAGGAATTTGCACAGCAGTTGTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TPS4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS315

100

100

1
  comX/sigX/comX1/sigX1 Streptococcus pyogenes JRS4

99.454

100

0.995
  comX/sigX/comX2/sigX2 Streptococcus pyogenes JRS4

99.454

100

0.995
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS8232

98.907

100

0.989
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS8232

98.907

100

0.989
  comX/sigX Streptococcus infantarius subsp. infantarius ATCC BAA-102

49.333

94.937

0.468
  comX/sigX Streptococcus mutans UA159

45.098

95.625

0.431
  comX/sigX Streptococcus suis isolate S10

40.645

99.359

0.404
  comX/sigX Streptococcus suis D9

40.645

99.359

0.404
  comX/sigX Streptococcus suis P1/7

40.645

99.359

0.404
  comX/sigX/comX2/sigX2 Streptococcus mitis SK321

42.105

95.597

0.403
  comX/sigX/comX2/sigX2 Streptococcus mitis NCTC 12261

41.447

95.597

0.396
  comR/comR1 Streptococcus gordonii str. Challis substr. CH1

40.909

96.25

0.394
  comR/comR2 Streptococcus gordonii str. Challis substr. CH1

40.909

96.25

0.394
  comX/sigX/comX1/sigX1 Streptococcus mitis SK321

40.132

95.597

0.384
  comX Streptococcus sobrinus strain NIDR 6715-7

45.455

84.153

0.383
  comX/sigX Streptococcus salivarius strain HSISS4

42

90.909

0.382
  comX Streptococcus salivarius SK126

42

90.909

0.382
  comX Streptococcus thermophilus LMD-9

42

90.909

0.382
  comX Streptococcus thermophilus LMG 18311

42

90.909

0.382
  comX/comX2 Streptococcus pneumoniae Rx1

39.216

96.226

0.377
  comX/comX1 Streptococcus pneumoniae Rx1

39.216

96.226

0.377
  comX/comX2 Streptococcus pneumoniae D39

39.216

96.226

0.377
  comX/comX1 Streptococcus pneumoniae D39

39.216

96.226

0.377
  comX/comX2 Streptococcus pneumoniae R6

39.216

96.226

0.377
  comX/comX1 Streptococcus pneumoniae R6

39.216

96.226

0.377
  comX/comX2 Streptococcus pneumoniae TIGR4

39.216

96.226

0.377
  comX/comX1 Streptococcus pneumoniae TIGR4

39.216

96.226

0.377
  comX/sigX/comX1/sigX1 Streptococcus mitis NCTC 12261

39.474

95.597

0.377

Multiple sequence alignment    



References

[1] Lauren Mashburn-Warren et al. (2012) The cryptic competence pathway in Streptococcus pyogenes is controlled by a peptide pheromone. Journal of Bacteriology 194(17):4589-600. [PMID: 22730123]