Detailed information    

experimental Experimentally validated

Overview

Name   comX/sigX   Type   Regulator
Locus tag   GPW51_RS00080 Genome accession   NZ_LR738721
Coordinates   15967..16437 (+) Length   156 a.a.
NCBI ID   WP_011921639.1    Uniprot ID   A0A123YDQ1
Organism   Streptococcus suis isolate S10     
Function   activate transcription of late competence genes   
Competence regulation

Function

A conserved CIN-box (YTACGAAYW), predicted to be bound by ComX, was found in the promoters of operons encoding genes involved in expression of the transformasome.


Genomic Context

Location: 10967..21437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW51_RS00065 tilS 11816..13084 (+) 1269 WP_011921636.1 tRNA lysidine(34) synthetase TilS -
  GPW51_RS00070 hpt 13092..13634 (+) 543 WP_011921637.1 hypoxanthine phosphoribosyltransferase -
  GPW51_RS00075 ftsH 13656..15629 (+) 1974 WP_011921638.1 ATP-dependent zinc metalloprotease FtsH -
  GPW51_RS00080 comX/sigX 15967..16437 (+) 471 WP_011921639.1 sigma-70 family RNA polymerase sigma factor Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX/sigX late competence genes positive effect
  comX/sigX late competence genes positive effect

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 19099.01 Da        Isoelectric Point: 8.9059

>NTDB_id=461 GPW51_RS00080 WP_011921639.1 15967..16437(+) (comX/sigX) [Streptococcus suis isolate S10]
MEFEKVYASVKGIVNKARKEFYIKLWDRDDWEQEGMMTLFELLEAQPWLVDEQVQLYCYFKVKFRNRIKDRIRKQESQKR
KFDRMPHEDIYELSHAIQSPGLINDELLMLRGALRDYRKNLSNDQLDKYEKLISGQCFNGRREMIRDLQIHLKDFR

Nucleotide


Download         Length: 471 bp        

>NTDB_id=461 GPW51_RS00080 WP_011921639.1 15967..16437(+) (comX/sigX) [Streptococcus suis isolate S10]
ATGGAATTCGAAAAAGTGTACGCAAGCGTCAAAGGTATTGTAAACAAGGCTCGAAAAGAGTTTTACATTAAACTATGGGA
TCGAGATGATTGGGAACAAGAAGGAATGATGACCTTGTTTGAATTGTTGGAAGCTCAACCGTGGCTAGTTGATGAACAAG
TTCAATTATATTGTTATTTTAAAGTCAAGTTCAGAAATCGAATCAAAGATCGTATCCGCAAACAGGAAAGTCAAAAACGC
AAGTTTGACCGTATGCCACATGAAGATATTTACGAATTATCTCACGCAATACAATCACCGGGATTGATAAACGATGAATT
ATTAATGTTGAGAGGTGCCTTGAGAGATTATCGAAAAAATCTGAGCAATGATCAACTTGATAAATACGAAAAATTAATTA
GCGGACAATGTTTTAATGGTCGCCGTGAAATGATACGTGATTTACAAATTCATTTGAAAGACTTTCGCTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A123YDQ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comX/sigX Streptococcus suis P1/7

100

100

1
  comX/sigX Streptococcus suis D9

99.359

100

0.994
  comR/comR2 Streptococcus gordonii str. Challis substr. CH1

49.032

99.359

0.487
  comR/comR1 Streptococcus gordonii str. Challis substr. CH1

49.032

99.359

0.487
  comX/comX2 Streptococcus pneumoniae TIGR4

46.358

96.795

0.449
  comX/comX1 Streptococcus pneumoniae TIGR4

46.358

96.795

0.449
  comX/sigX/comX2/sigX2 Streptococcus mitis NCTC 12261

45.752

98.077

0.449
  comX/sigX/comX2/sigX2 Streptococcus mitis SK321

45.098

98.077

0.442
  comX/sigX/comX1/sigX1 Streptococcus mitis SK321

45.098

98.077

0.442
  comX/comX2 Streptococcus pneumoniae D39

45.695

96.795

0.442
  comX/comX2 Streptococcus pneumoniae Rx1

45.695

96.795

0.442
  comX/comX1 Streptococcus pneumoniae Rx1

45.695

96.795

0.442
  comX/comX1 Streptococcus pneumoniae D39

45.695

96.795

0.442
  comX/comX2 Streptococcus pneumoniae R6

45.695

96.795

0.442
  comX/comX1 Streptococcus pneumoniae R6

45.695

96.795

0.442
  comX/sigX/comX1/sigX1 Streptococcus mitis NCTC 12261

44.444

98.077

0.436
  comX/sigX Streptococcus mutans UA159

43.226

99.359

0.429
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS315

40.645

99.359

0.404
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS315

40.645

99.359

0.404
  comX Streptococcus thermophilus LMD-9

40.523

98.077

0.397
  comX Streptococcus thermophilus LMG 18311

40.523

98.077

0.397
  comX Streptococcus sobrinus strain NIDR 6715-7

40.523

98.077

0.397
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS8232

40

99.359

0.397
  comX/sigX/comX2/sigX2 Streptococcus pyogenes JRS4

40

99.359

0.397
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS8232

40

99.359

0.397
  comX/sigX/comX1/sigX1 Streptococcus pyogenes JRS4

40

99.359

0.397
  comX/sigX Streptococcus infantarius subsp. infantarius ATCC BAA-102

38.854

100

0.391
  comX Streptococcus salivarius SK126

39.869

98.077

0.391
  comX/sigX Streptococcus salivarius strain HSISS4

39.869

98.077

0.391

Multiple sequence alignment    



References

[1] Edoardo Zaccaria et al. (2014) Control of competence for DNA transformation in streptococcus suis by genetically transferable pherotypes. PloS One 9(6):e99394. [PMID: 24968201]