Detailed information    

experimental Experimentally validated

Overview

Name   prx   Type   Regulator
Locus tag   SPYM3_RS07355 Genome accession   NC_004070
Coordinates   1410725..1410907 (-) Length   60 a.a.
NCBI ID   WP_011054856.1    Uniprot ID   A0A0H2UW47
Organism   Streptococcus pyogenes MGAS315     
Function   Inhibit ComR activation   
Competence regulation

Function

In vitro experiments demonstrate that Prx binds ComR directly and prevents the ComR-XIP complex from interacting with DNA. Mutations of prx in vivo caused increased expression of the late competence gene ssb when induced with XIP as compared to wild-type, and Prx orthologues are able to inhibit ComR activation by XIP in a reporter strain which lacks an endogenous prx. An X-ray crystal structure of Prx reveals a unique fold that implies a novel molecular mechanism to inhibit ComR.


Genomic Context

Location: 1405725..1415907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYM3_RS07330 (SpyM3_1402) ftsL 1406659..1406982 (-) 324 WP_002988895.1 cell division protein FtsL -
  SPYM3_RS07335 (SpyM3_1403) rsmH 1406987..1407937 (-) 951 WP_002988893.1 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH -
  SPYM3_RS10405 (SpyM3_1404) - 1408024..1408188 (-) 165 WP_002994245.1 hypothetical protein -
  SPYM3_RS07340 (SpyM3_1405) - 1408290..1408451 (-) 162 WP_159316501.1 hypothetical protein -
  SPYM3_RS07345 (SpyM3_1406) - 1408471..1409721 (-) 1251 WP_011054854.1 glutamate-5-semialdehyde dehydrogenase -
  SPYM3_RS07350 (SpyM3_1407) proB 1409714..1410535 (-) 822 WP_011054855.1 glutamate 5-kinase -
  SPYM3_RS07355 (SpyM3_1408) prx 1410725..1410907 (-) 183 WP_011054856.1 hypothetical protein Regulator
  SPYM3_RS07360 (SpyM3_1409) - 1411140..1412126 (+) 987 WP_048901986.1 DNA/RNA non-specific endonuclease -
  SPYM3_RS07365 (SpyM3_1410) - 1412441..1412755 (-) 315 WP_228358673.1 hypothetical protein -
  SPYM3_RS07370 - 1413077..1414279 (-) 1203 Protein_1427 glucosaminidase domain-containing protein -
  SPYM3_RS07380 (SpyM3_1413) - 1414395..1414622 (-) 228 WP_003058873.1 phage holin -
  SPYM3_RS07385 (SpyM3_1414) - 1414619..1414891 (-) 273 WP_002986916.1 DUF7365 family protein -
  SPYM3_RS07390 (SpyM3_1415) - 1414901..1415518 (-) 618 WP_011054861.1 DUF1366 domain-containing protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  prx comR negative effect
  comR comX/sigX/comX2/sigX2 positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comR comX/sigX/comX1/sigX1 positive effect
  comS comX/sigX/comX1/sigX1 positive effect
  clpP comX/sigX/comX1/sigX1 negative effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comS comX/sigX/comX2/sigX2 positive effect
  clpP comX/sigX/comX2/sigX2 negative effect

Sequence

Protein


Download         Length: 60 a.a.        Molecular weight: 6927.67 Da        Isoelectric Point: 3.9286

>NTDB_id=549 SPYM3_RS07355 WP_011054856.1 1410725..1410907(-) (prx) [Streptococcus pyogenes MGAS315]
MLTYDEFKQAIDDGYITGDTVAIVRKNGQIFDYALPNEEVRNGEVVTYENVEEVLRELDK

Nucleotide


Download         Length: 183 bp        

>NTDB_id=549 SPYM3_RS07355 WP_011054856.1 1410725..1410907(-) (prx) [Streptococcus pyogenes MGAS315]
ATGCTAACATACGATGAGTTTAAGCAAGCTATTGATGACGGATATATCACAGGCGACACAGTGGCGATCGTGCGCAAAAA
CGGACAGATTTTTGATTATGCGTTGCCGAATGAAGAGGTAAGAAATGGGGAGGTTGTAACATACGAAAATGTGGAAGAAG
TGCTGAGGGAATTAGACAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2UW47

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  prx Streptococcus pyogenes MGAS315

84.746

95.161

0.806
  prx Streptococcus pyogenes MGAS8232

80

100

0.8
  prx Streptococcus pyogenes MGAS315

80

100

0.8
  prx Streptococcus pyogenes MGAS315

78.333

100

0.783
  prx Streptococcus pyogenes MGAS315

75

100

0.75
  prx Streptococcus pyogenes MGAS315

75

100

0.75

Multiple sequence alignment    



References

[1] Lauren Mashburn-Warren et al. (2018) The conserved mosaic prophage protein paratox inhibits the natural competence regulator ComR in Streptococcus. Scientific Reports 8(1):16535. [PMID: 30409983]