Detailed information    

experimental Experimentally validated

Overview

Name   prx   Type   Regulator
Locus tag   SPYM3_RS06785 Genome accession   NC_004070
Coordinates   1313282..1313464 (-) Length   60 a.a.
NCBI ID   WP_011054793.1    Uniprot ID   A0A5S4TJP1
Organism   Streptococcus pyogenes MGAS315     
Function   Inhibit ComR activation   
Competence regulation

Function

In vitro experiments demonstrate that Prx binds ComR directly and prevents the ComR-XIP complex from interacting with DNA. Mutations of prx in vivo caused increased expression of the late competence gene ssb when induced with XIP as compared to wild-type, and Prx orthologues are able to inhibit ComR activation by XIP in a reporter strain which lacks an endogenous prx. An X-ray crystal structure of Prx reveals a unique fold that implies a novel molecular mechanism to inhibit ComR.


Genomic Context

Location: 1308282..1318464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYM3_RS06775 (SpyM3_1298) rocA 1309717..1311071 (-) 1355 Protein_1314 transcriptional regulator RocA -
  SPYM3_RS06780 (SpyM3_1299) rlmD 1311736..1313091 (-) 1356 WP_002991789.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
  SPYM3_RS06785 (SpyM3_1300) prx 1313282..1313464 (-) 183 WP_011054793.1 hypothetical protein Regulator
  SPYM3_RS06790 (SpyM3_1301) speA 1313684..1314439 (+) 756 WP_011054794.1 streptococcal pyrogenic exotoxin SpeA -
  SPYM3_RS06795 (SpyM3_1302) - 1314561..1315220 (-) 660 WP_009880240.1 hypothetical protein -
  SPYM3_RS06800 (SpyM3_1303) - 1315220..1315441 (-) 222 WP_009880241.1 hypothetical protein -
  SPYM3_RS06805 (SpyM3_1304) - 1315451..1316224 (-) 774 WP_011054795.1 hypothetical protein -
  SPYM3_RS06810 (SpyM3_1305) - 1316235..1316837 (-) 603 WP_011054796.1 hypothetical protein -
  SPYM3_RS06815 (SpyM3_1306) - 1316849..1317613 (-) 765 WP_011054797.1 CHAP domain-containing protein -
  SPYM3_RS06820 (SpyM3_1307) - 1317615..1317947 (-) 333 WP_011054798.1 phage holin -
  SPYM3_RS10645 (SpyM3_1308) - 1318283..1318405 (-) 123 WP_015055953.1 hypothetical protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  prx comR negative effect
  comR comX/sigX/comX2/sigX2 positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comR comX/sigX/comX1/sigX1 positive effect
  comS comX/sigX/comX1/sigX1 positive effect
  clpP comX/sigX/comX1/sigX1 negative effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comS comX/sigX/comX2/sigX2 positive effect
  clpP comX/sigX/comX2/sigX2 negative effect

Sequence

Protein


Download         Length: 60 a.a.        Molecular weight: 7014.08 Da        Isoelectric Point: 4.3313

>NTDB_id=547 SPYM3_RS06785 WP_011054793.1 1313282..1313464(-) (prx) [Streptococcus pyogenes MGAS315]
MLYIDEFKEAIDKGYILGDTVAIVRKNGKIFDYVLPHEKVRDDEVVTVERVEEVMVELDK

Nucleotide


Download         Length: 183 bp        

>NTDB_id=547 SPYM3_RS06785 WP_011054793.1 1313282..1313464(-) (prx) [Streptococcus pyogenes MGAS315]
ATGTTATATATAGATGAGTTTAAAGAAGCGATTGATAAGGGGTATATTTTAGGTGACACAGTAGCGATAGTGCGTAAAAA
CGGAAAGATATTTGATTATGTGTTACCACACGAAAAAGTGAGAGATGATGAAGTTGTGACGGTAGAGAGAGTGGAAGAAG
TGATGGTGGAATTAGACAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  prx Streptococcus pyogenes MGAS315

78.333

100

0.783
  prx Streptococcus pyogenes MGAS315

76.667

100

0.767
  prx Streptococcus pyogenes MGAS315

75

100

0.75
  prx Streptococcus pyogenes MGAS8232

75

100

0.75
  prx Streptococcus pyogenes MGAS315

68.333

100

0.683
  prx Streptococcus pyogenes MGAS315

76.19

70

0.533

Multiple sequence alignment    



References

[1] Lauren Mashburn-Warren et al. (2018) The conserved mosaic prophage protein paratox inhibits the natural competence regulator ComR in Streptococcus. Scientific Reports 8(1):16535. [PMID: 30409983]