Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   HI_0985 Genome accession   L42023
Coordinates   1041677..1042798 (-) Length   373 a.a.
NCBI ID   AAC22646.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

The dprA deletion mutant had transformation defect and normal DNA uptake.


Genomic Context

Location: 1036677..1047798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0979 - 1037121..1038113 (+) 993 AAC22639.1 nitrogen fixation protein (nifR3) -
  HI_0980 - 1038094..1038393 (+) 300 AAC22640.1 Hin recombinational enhancer binding protein (fis) -
  HI_0981 - 1038488..1038973 (-) 486 AAC22643.1 small protein B (smpB) -
  HI_0982 - 1039212..1040177 (-) 966 AAC22644.1 6-phosphofructokinase (pfkA) -
  HI_0983 - 1040273..1040854 (-) 582 AAC22652.1 predicted coding region HI0983 -
  HI_0984 - 1040847..1041665 (-) 819 AAC22645.1 conserved hypothetical protein -
  HI_0985 dprA 1041677..1042798 (-) 1122 AAC22646.1 DNA processing chain A (dprA) Machinery gene
  HI_0986 - 1043307..1044902 (+) 1596 AAC22647.1 2-isopropylmalate synthase (leuA) -
  HI_0987 - 1044988..1046064 (+) 1077 AAC22648.1 3-isopropylmalate dehydrogenase (beta-IPM dehydrogenase) (leuB) -
  HI_0988 - 1046240..1047649 (+) 1410 AAC22649.1 3-isopropylmalate dehydratase, alpha subunit (leuC) -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sxy/tfoX dprA positive effect

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41639.79 Da        Isoelectric Point: 6.7679

>NTDB_id=1361 HI_0985 AAC22646.1 1041677..1042798(-) (dprA) [Haemophilus influenzae Rd KW20]
MNDITYTLLRLMQVPKLGGVGIDKILSNITLNELLNYDDVAFRQMGWGAIQIRRWFKPEAKFIEPALVWSQKEGNHLVNY
FSPFYPFLLKQTASFPPLLFVKGNLTALSQRQMAMVGSRYCTTYGEYWAKHFATELSLAGFTITSGLALGIDGHCHQAVV
NIQGQTIAVLGSGLEQIYPSKHQRLSAQIIENNGALVSEFLPNQAPIAANFPRRNRIISGLSVGTLVVEATEKSGSLITA
RYALEQNREVFAVPGNIQNKSSQGCHRLIKQGAMLVENAKDILETLYQHSIHSQTEIDFDQIAVPNYTPPPDPRRLVEAP
SHPKLYSRIGYTPVSIDDLAEEFNLSVDVLLVQLLDLELQDLIISENGLYKRV

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1361 HI_0985 AAC22646.1 1041677..1042798(-) (dprA) [Haemophilus influenzae Rd KW20]
ATGAATGACATTACCTACACATTACTCCGTTTAATGCAAGTGCCTAAACTTGGCGGCGTTGGCATTGATAAAATTTTGTC
CAATATTACCTTAAATGAATTACTTAATTATGATGATGTTGCTTTCCGACAAATGGGGTGGGGAGCCATCCAAATTCGCC
GTTGGTTCAAGCCTGAAGCAAAATTTATTGAGCCTGCACTTGTGTGGTCACAAAAAGAGGGAAATCATTTAGTGAATTAT
TTTTCCCCTTTTTATCCCTTTTTGTTGAAACAGACCGCAAGTTTTCCGCCACTATTATTTGTAAAAGGAAATTTAACCGC
ACTTTCACAACGCCAAATGGCAATGGTTGGAAGCCGTTATTGCACGACTTACGGAGAATATTGGGCAAAACATTTTGCGA
CAGAACTTTCATTAGCTGGCTTTACGATTACTAGTGGACTTGCATTAGGAATTGATGGACATTGCCATCAAGCTGTGGTC
AATATTCAAGGACAAACTATTGCGGTTTTAGGCAGTGGATTAGAACAAATCTATCCCTCTAAACATCAAAGATTATCGGC
ACAAATCATCGAAAATAATGGCGCATTAGTCTCTGAGTTCTTACCAAACCAAGCCCCCATTGCCGCTAATTTCCCACGTC
GTAATCGGATTATTAGCGGGCTTTCAGTCGGCACGCTTGTTGTTGAAGCCACAGAAAAAAGCGGCTCGCTTATTACTGCG
CGATATGCGTTGGAACAAAACCGAGAGGTTTTTGCTGTGCCGGGAAACATTCAAAATAAATCAAGTCAAGGTTGTCATCG
CCTAATTAAACAAGGTGCAATGTTGGTAGAAAATGCAAAGGACATTTTGGAAACTCTCTACCAACATTCAATCCATAGCC
AAACTGAAATTGATTTTGACCAGATCGCCGTGCCTAATTACACGCCACCACCTGATCCTCGTCGATTAGTAGAAGCACCA
AGCCACCCGAAACTTTATTCGCGTATTGGTTACACGCCAGTGAGCATTGATGATTTAGCGGAAGAATTTAATTTAAGCGT
CGATGTTTTGCTTGTTCAGTTGCTCGATCTTGAGTTGCAAGATCTTATCATAAGTGAAAATGGGTTATATAAACGCGTTT
AG

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(311-366)

DNA recombination-mediator protein A (DNA_processg_A)

(71-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Glaesserella parasuis strain SC1401

52.011

93.298

0.485
  dprA Vibrio campbellii strain DS40M4

43.617

100

0.44
  dprA Vibrio cholerae strain A1552

43.288

97.855

0.424
  dprA Legionella pneumophila strain ERS1305867

40.634

93.029

0.378
  dprA Lactococcus lactis subsp. cremoris KW2

39.259

95.745

0.376
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

38.202

94.681

0.362
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

38.202

94.681

0.362
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

38.202

94.681

0.362
  dprA/cilB/dalA Streptococcus pneumoniae D39

38.202

94.681

0.362
  dprA/cilB/dalA Streptococcus pneumoniae R6

38.202

94.681

0.362

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]
[2] S Karudapuram et al. (1995) DNA sequence and characterization of Haemophilus influenzae dprA+, a gene required for chromosomal but not plasmid DNA transformation. Journal of Bacteriology 177(11):3235-40. [PMID: 7768823]