Detailed information NTG0001375

Experimentally validated

Overview

• Name: sxy/tfoX
• Type: Regulator
• Locus tag: HI_0601
• Genome accession: L42023
• Coordinates: 622876..623529 (+)
• Length: 217 a.a.
• NCBI ID: AAC22258.1
• Uniprot ID: -
• Organism: Haemophilus influenzae Rd KW20
• Function: positive regulator of competence gene Competence regulation

Function

The essential competence gene sxy is induced early in competence development and is required for competence-induced transcription of CRE-regulon genes but not other CRP-regulated genes, suggesting that Sxy may act as an accessory factor directing CRP to CRE sites.

Genomic Context

Location: 617876..628529

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HI_0596	-	618348..619352 (-)	1005	AAC22253.1	ornithine carbamoyltransferase (arcB)	-
HI_0597	-	619754..620572 (+)	819	AAC22254.1	conserved hypothetical protein	-
HI_0597.1	-	620572..621054 (+)	483	AAC22255.1	crcB protein	-
HI_0599	-	620954..621334 (-)	381	AAC22256.1	regulatory protein (recX)	-
HI_0600	recA	621492..622556 (-)	1065	AAC22257.1	recA protein (recA)	Machinery gene
HI_0601	sxy/tfoX	622876..623529 (+)	654	AAC22258.1	DNA transformation protein (tfoX)	Regulator

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
sxy/tfoX	dprA	positive effect

Sequence

Protein

• Length: 217 a.a.
• Molecular weight: 25074.43 Da
• Isoelectric Point: 10.0171

>NTDB_id=1375 HI_0601 AAC22258.1 622876..623529(+) (sxy/tfoX) [Haemophilus influenzae Rd KW20]
MNIKDEHIDSVCSLLDQLVGNVSFKNLFTGYGLFHKEETMFAIWQNKKLYLRGEGVLAIQLTKLGCEPFTTNELNKRFVL
SQYYALSDQILRSNRLCRKLIILSIKQILEQKLECTLRKLNRLKDLPNLTIKHERALIKVGITNVAMLREIGAENALVEL
KKSGSGATLDFYWKLVCALQNKNSQMLSQAEKERLLKKLNEVWRKNGLKGYRKLDDE

Nucleotide

• Length: 654 bp

>NTDB_id=1375 HI_0601 AAC22258.1 622876..623529(+) (sxy/tfoX) [Haemophilus influenzae Rd KW20]
ATGAATATAAAGGATGAGCATATAGATAGCGTTTGCTCCTTGTTAGATCAGTTAGTAGGAAATGTTTCCTTTAAAAATCT
TTTTACTGGTTACGGTTTGTTTCACAAGGAAGAGACAATGTTTGCTATTTGGCAAAATAAAAAACTTTATTTACGCGGTG
AGGGTGTTCTCGCAATTCAATTAACTAAATTAGGTTGTGAACCTTTTACAACGAATGAATTGAATAAACGGTTTGTGCTT
TCACAATATTATGCACTTTCTGATCAGATTTTACGTAGTAATAGATTGTGTAGAAAATTGATTATTCTTTCTATTAAGCA
GATTCTTGAGCAGAAGCTAGAATGTACGTTAAGAAAATTGAATCGATTAAAGGATTTACCCAATTTAACGATTAAACATG
AAAGAGCTTTAATAAAAGTTGGTATTACAAATGTTGCGATGCTAAGAGAGATTGGCGCAGAAAATGCATTGGTGGAATTA
AAGAAAAGTGGCAGTGGTGCTACGCTTGATTTTTATTGGAAATTAGTATGTGCTTTGCAAAATAAAAATAGTCAGATGTT
AAGTCAAGCTGAAAAAGAGCGTTTATTGAAGAAATTAAACGAAGTTTGGAGAAAAAATGGCTTAAAAGGCTATAGAAAAT
TAGATGATGAATAA

Domains

Predicted by InterproScan.

• TfoX C-terminal domain (TfoX_C) (121-198)

• TfoX N-terminal domain (TfoX_N) (14-107)

References

• [1]: Andrew D S Cameron et al. (2008) CRP binding and transcription activation at CRP-S sites. Journal of Molecular Biology 383(2):313-23. [PMID: 18761017]
• [2]: Rosemary J Redfield et al. (2005) A novel CRP-dependent regulon controls expression of competence genes in Haemophilus influenzae. Journal of Molecular Biology 347(4):735-47. [PMID: 15769466]
• [3]: S Karudapuram et al. (1997) The Haemophilus influenzae dprABC genes constitute a competence-inducible operon that requires the product of the tfoX (sxy) gene for transcriptional activation. Journal of Bacteriology 179(15):4815-20. [PMID: 9244270]
• [4]: J J Zulty et al. (1995) Identification of a DNA transformation gene required for com101A+ expression and supertransformer phenotype in Haemophilus influenzae. Proceedings of The National Academy of Sciences of The United States of America 92(8):3616-20. [PMID: 7724607]
• [5]: P M Williams et al. (1994) The Haemophilus influenzae sxy-1 mutation is in a newly identified gene essential for competence. Journal of Bacteriology 176(22):6789-94. [PMID: 7961436]
• [6]: R J Redfield (1991) sxy-1, a Haemophilus influenzae mutation causing greatly enhanced spontaneous competence. Journal of Bacteriology 173(18):5612-8. [PMID: 1653215]