Detailed information NTG0000270

Experimentally validated

Overview

• Name: comGA/cglA/cilD
• Type: Machinery gene
• Locus tag: KZH43_RS09265
• Genome accession: NZ_CP079923
• Coordinates: 1826025..1826966 (-)
• Length: 313 a.a.
• NCBI ID: WP_000249564.1
• Uniprot ID: Q8DN86
• Organism: Streptococcus pneumoniae Rx1
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus DNA binding and uptake

Function

The comGA gene is essential for the initial binding of transforming DNA to the cell surface and for the assembly of the pseudopilus, a structure required for DNA uptake.

Genomic Context

Location: 1821025..1831966

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KZH43_RS09225 (KZH43_09220)	comYH	1821511..1822464 (-)	954	WP_000345135.1	class I SAM-dependent methyltransferase	Machinery gene
KZH43_RS09230 (KZH43_09225)	-	1822525..1823119 (-)	595	Protein_1826	class I SAM-dependent methyltransferase	-
KZH43_RS09235 (KZH43_09230)	comGG/cglG	1823256..1823669 (-)	414	WP_000265622.1	competence type IV pilus minor pilin ComGG	Machinery gene
KZH43_RS09240 (KZH43_09235)	comGF/cglF	1823647..1824108 (-)	462	WP_000250534.1	competence type IV pilus minor pilin ComGF	Machinery gene
KZH43_RS09245 (KZH43_09240)	comGE/cglE	1824071..1824373 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
KZH43_RS09250 (KZH43_09245)	comGD/cglD	1824336..1824740 (-)	405	WP_000588026.1	competence type IV pilus minor pilin ComGD	Machinery gene
KZH43_RS09255 (KZH43_09250)	comGC/cglC	1824733..1825059 (-)	327	WP_000738626.1	comG operon protein ComGC	Machinery gene
KZH43_RS09260 (KZH43_09255)	comGB/cglB	1825061..1826077 (-)	1017	WP_074196785.1	competence type IV pilus assembly protein ComGB	Machinery gene
KZH43_RS09265 (KZH43_09260)	comGA/cglA/cilD	1826025..1826966 (-)	942	WP_000249564.1	competence type IV pilus ATPase ComGA	Machinery gene
KZH43_RS09270 (KZH43_09265)	-	1827042..1827407 (-)	366	WP_000286415.1	DUF1033 family protein	-
KZH43_RS09275 (KZH43_09270)	-	1827558..1828616 (-)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-
KZH43_RS09280 (KZH43_09275)	nagA	1828779..1829930 (-)	1152	WP_001134457.1	N-acetylglucosamine-6-phosphate deacetylase	-
KZH43_RS09285 (KZH43_09280)	-	1830083..1831900 (-)	1818	WP_001220853.1	acyltransferase family protein	-

Sequence

Protein

• Length: 313 a.a.
• Molecular weight: 35555.47 Da
• Isoelectric Point: 6.1330

>NTDB_id=270 KZH43_RS09265 WP_000249564.1 1826025..1826966(-) (comGA/cglA/cilD) [Streptococcus pneumoniae Rx1]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDHKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide

• Length: 942 bp

>NTDB_id=270 KZH43_RS09265 WP_000249564.1 1826025..1826966(-) (comGA/cglA/cilD) [Streptococcus pneumoniae Rx1]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCATAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (9-266)

3D structure

• AlphaFold DB: Q8DN86

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	100	100	1
comGA/cglA/cilD	Streptococcus pneumoniae D39	100	100	1
comGA/cglA/cilD	Streptococcus pneumoniae R6	100	100	1
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	95.847	100	0.958
comYA	Streptococcus gordonii str. Challis substr. CH1	78.065	99.042	0.773
comYA	Streptococcus mutans UA159	65.916	99.361	0.655
comYA	Streptococcus mutans UA140	65.916	99.361	0.655
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	62.581	99.042	0.62
comGA	Lactococcus lactis subsp. cremoris KW2	54.808	99.681	0.546
comGA	Latilactobacillus sakei subsp. sakei 23K	41.912	91.892	0.385

References

• [1]: Cheng-Foh Le et al. (2016) Transcriptome analysis of Streptococcus pneumoniae treated with the designed antimicrobial peptides, DM3. Scientific Reports 6:26828. [PMID: 27225022]
• [2]: Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]
• [3]: Ping Luo et al. (2003) Transient association of an alternative sigma factor, ComX, with RNA polymerase during the period of competence for genetic transformation in Streptococcus pneumoniae. Journal of Bacteriology 185(1):349-58. [PMID: 12486073]
• [4]: Ping Luo et al. (2003) ComX is a unique link between multiple quorum sensing outputs and competence in Streptococcus pneumoniae. Molecular Microbiology 50(2):623-33. [PMID: 14617184]
• [5]: Mathieu Bergé et al. (2002) Uptake of transforming DNA in Gram-positive bacteria: a view from Streptococcus pneumoniae. Molecular Microbiology 45(2):411-21. [PMID: 12123453]