Detailed information    

experimental Experimentally validated

Overview

Name   comGG/cglG   Type   Machinery gene
Locus tag   SPD_RS09865 Genome accession   NC_008533
Coordinates   1839322..1839735 (-) Length   137 a.a.
NCBI ID   WP_000265622.1    Uniprot ID   Q8CY84
Organism   Streptococcus pneumoniae D39     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

ComGG gene is a component of the competence pseudopilus machinery. Pneumococcal ComGG stabilizes the minor pilin ComGD and ComGF and can interact with and stabilize the major pilin ComGC.


Genomic Context

Location: 1834322..1844735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPD_RS09840 (SPD_1850) - 1835017..1835841 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  SPD_RS09845 (SPD_1851) rnpA 1835816..1836187 (-) 372 WP_000739246.1 ribonuclease P protein component -
  SPD_RS11950 (SPD_1852) - 1836204..1836335 (-) 132 WP_000768904.1 hypothetical protein -
  SPD_RS09850 (SPD_1853) - 1836336..1837526 (-) 1191 WP_000167757.1 acetate kinase -
  SPD_RS09855 (SPD_1854) comYH 1837577..1838530 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  SPD_RS09860 - 1838591..1839185 (-) 595 Protein_1835 class I SAM-dependent methyltransferase -
  SPD_RS09865 (SPD_1857) comGG/cglG 1839322..1839735 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  SPD_RS09870 (SPD_1858) comGF/cglF 1839713..1840174 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPD_RS09875 (SPD_1859) comGE/cglE 1840137..1840439 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SPD_RS09880 (SPD_1860) comGD/cglD 1840402..1840806 (-) 405 WP_000588026.1 competence type IV pilus minor pilin ComGD Machinery gene
  SPD_RS09885 (SPD_1861) comGC/cglC 1840799..1841125 (-) 327 WP_000738626.1 comG operon protein ComGC Machinery gene
  SPD_RS09890 (SPD_1862) comGB/cglB 1841127..1842143 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPD_RS09895 (SPD_1863) comGA/cglA/cilD 1842091..1843032 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  SPD_RS09900 (SPD_1864) - 1843108..1843473 (-) 366 WP_000286412.1 DUF1033 family protein -
  SPD_RS09905 (SPD_1865) - 1843624..1844682 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 16003.46 Da        Isoelectric Point: 10.2472

>NTDB_id=235 SPD_RS09865 WP_000265622.1 1839322..1839735(-) (comGG/cglG) [Streptococcus pneumoniae D39]
MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNKEKLVAFAMAKRTKDKVEQESGEQFFNLGQVSYQNKK
TGLVTRVRTDKSQYEFLFPSVKIKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS

Nucleotide


Download         Length: 414 bp        

>NTDB_id=235 SPD_RS09865 WP_000265622.1 1839322..1839735(-) (comGG/cglG) [Streptococcus pneumoniae D39]
GTGTGGAAAAAGAAAAAAGTTAAGGCAGGTGTTCTCCTCTACGCAGTCACCATAGCAGCCATCTTTAGTCTTTTGTTGCA
ATTTTATTTGAACCGACAAGTCGCCCACTATCAAGACTATGCTTTGAATAAAGAAAAATTGGTTGCTTTTGCTATGGCTA
AACGAACCAAAGATAAGGTTGAGCAAGAAAGTGGGGAACAGTTTTTTAATCTAGGTCAGGTAAGCTATCAAAACAAGAAA
ACTGGCTTAGTGACGAGGGTTCGTACGGATAAGAGCCAATATGAGTTTCTGTTTCCTTCAGTCAAAATCAAAGAAGAGAA
AAGAGATAAAAAGGAAGAGGTAGCGACCGATTCAAGCGAAAAAGTGGAGAAGAAAAAATCAGAAGAGAAGCCTGAAAAGA
AAGAGAATTCATAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8CY84

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGG/cglG Streptococcus pneumoniae Rx1

100

100

1
  comGG/cglG Streptococcus pneumoniae R6

100

100

1
  comGG/cglG Streptococcus pneumoniae TIGR4

100

100

1
  comGG/cglG Streptococcus mitis SK321

87.591

100

0.876
  comGG/cglG Streptococcus mitis NCTC 12261

89.256

88.321

0.788
  comYG Streptococcus mutans UA159

38.806

97.81

0.38
  comYG Streptococcus mutans UA140

38.806

97.81

0.38

Multiple sequence alignment    



References

[1] Vitor Oliveira et al. (2021) The Role of Minor Pilins in Assembly and Function of the Competence Pilus of Streptococcus pneumoniae. Frontiers in Cellular And Infection Microbiology 11:808601. [PMID: 35004361]
[2] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]