Detailed information    

experimental Experimentally validated

Overview

Name   comGE/cglE   Type   Machinery gene
Locus tag   SMSK321_RS09220 Genome accession   NZ_AEDT01000028
Coordinates   19204..19506 (-) Length   100 a.a.
NCBI ID   WP_255262223.1    Uniprot ID   -
Organism   Streptococcus mitis SK321     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 14204..24506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMSK321_RS09195 (SMSK321_1264) rnpA 14993..15364 (-) 372 WP_000739245.1 ribonuclease P protein component -
  SMSK321_RS09200 (SMSK321_1265) - 15514..16704 (-) 1191 WP_000167794.1 acetate kinase -
  SMSK321_RS09205 (SMSK321_1266) comYH 16755..17708 (-) 954 WP_000345131.1 class I SAM-dependent methyltransferase Machinery gene
  SMSK321_RS09210 (SMSK321_1267) - 17769..18356 (-) 588 WP_000679788.1 class I SAM-dependent methyltransferase -
  SMSK321_RS0102610 comGG/cglG 18389..18802 (-) 414 WP_000265640.1 competence type IV pilus minor pilin ComGG Machinery gene
  SMSK321_RS09215 (SMSK321_1268) comGF/cglF 18780..19241 (-) 462 WP_000250512.1 competence type IV pilus minor pilin ComGF Machinery gene
  SMSK321_RS09220 (SMSK321_1269) comGE/cglE 19204..19506 (-) 303 WP_255262223.1 competence type IV pilus minor pilin ComGE Machinery gene
  SMSK321_RS09225 (SMSK321_1270) comGD/cglD 19469..19873 (-) 405 WP_000588032.1 competence type IV pilus minor pilin ComGD Machinery gene
  SMSK321_RS09230 (SMSK321_1271) comGC/cglC 19866..20192 (-) 327 WP_000738643.1 comG operon protein ComGC Machinery gene
  SMSK321_RS09235 (SMSK321_1272) comGB/cglB 20194..21210 (-) 1017 WP_080550783.1 competence type IV pilus assembly protein ComGB Machinery gene
  SMSK321_RS09240 (SMSK321_1273) comGA/cglA/cilD 21158..22100 (-) 943 Protein_25 competence type IV pilus ATPase ComGA Machinery gene
  SMSK321_RS09245 (SMSK321_1274) - 22177..22542 (-) 366 WP_000286410.1 DUF1033 family protein -
  SMSK321_RS09250 (SMSK321_1275) - 22693..23751 (-) 1059 WP_004235578.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 100 a.a.        Molecular weight: 11163.21 Da        Isoelectric Point: 10.0522

>NTDB_id=532 SMSK321_RS09220 WP_255262223.1 19204..19506(-) (comGE/cglE) [Streptococcus mitis SK321]
MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEILRVAKMALQTGQNQVKINGVEIQVFS
SEKGLEVYHGSEQLLAIKEP

Nucleotide


Download         Length: 303 bp        

>NTDB_id=532 SMSK321_RS09220 WP_255262223.1 19204..19506(-) (comGE/cglE) [Streptococcus mitis SK321]
ATGGAAAAATTAAACGCATTAAGGAAACAAAAAATTAGGGCAGTGATTTTACTGGAAGCAGTCGTCGCTCTAGCTATCTT
TGCCAGCATTGCGACCCTTCTTTTGGGACAAATTCAGAAAAATAGGCAAGAAGAAGCAAAAATCTTGCAAAAGGAAGAAA
TCTTGAGGGTAGCTAAGATGGCCTTGCAGACAGGTCAAAATCAGGTAAAGATAAACGGAGTTGAGATTCAGGTGTTTTCT
AGTGAAAAGGGATTGGAGGTCTACCATGGTTCAGAACAGTTGTTGGCTATCAAAGAGCCATAA

Domains

Predicted by InterproScan.

Type II secretory pathway pseudopilin (ComGE)

(16-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE/cglE Streptococcus mitis NCTC 12261

99

100

0.99
  comGE/cglE Streptococcus pneumoniae R6

98

100

0.98
  comGE/cglE Streptococcus pneumoniae TIGR4

98

100

0.98
  comGE/cglE Streptococcus pneumoniae Rx1

98

100

0.98
  comGE/cglE Streptococcus pneumoniae D39

98

100

0.98
  comYE Streptococcus mutans UA159

44.444

92.784

0.412
  comYE Streptococcus mutans UA140

44.444

92.784

0.412

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]