Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   TT_RS09535 Genome accession   NC_005835
Coordinates   1775457..1776461 (-) Length   334 a.a.
NCBI ID   WP_011174226.1    Uniprot ID   Q72GH5
Organism   Thermus thermophilus HB27     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

DprA initiates the integration of the incoming DNA into the chromosome by loading RecA onto ssDNA.


Genomic Context

Location: 1770457..1781461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TT_RS09500 (TT_C1866) hisG 1770929..1771549 (+) 621 WP_011174220.1 ATP phosphoribosyltransferase -
  TT_RS09505 (TT_C1867) trmH 1771546..1772130 (+) 585 WP_011174221.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  TT_RS09510 (TT_C1868) - 1772120..1772359 (-) 240 WP_008630920.1 hypothetical protein -
  TT_RS09515 (TT_C1869) - 1772361..1772549 (-) 189 WP_011174222.1 hypothetical protein -
  TT_RS09520 (TT_C1870) - 1772539..1773495 (-) 957 WP_011174223.1 branched-chain amino acid transaminase -
  TT_RS09525 (TT_C1871) - 1773482..1774435 (-) 954 WP_011174224.1 hydrogen peroxide-inducible genes activator -
  TT_RS09530 (TT_C1872) - 1774545..1775453 (+) 909 WP_011174225.1 manganese catalase family protein -
  TT_RS09535 (TT_C1873) dprA 1775457..1776461 (-) 1005 WP_011174226.1 DNA-processing protein DprA Machinery gene
  TT_RS09540 (TT_C1874) era 1776546..1777451 (+) 906 WP_008630929.1 GTPase Era -
  TT_RS09545 (TT_C1875) - 1777496..1778383 (+) 888 WP_011174227.1 acyltransferase -
  TT_RS09555 (TT_C1876) - 1778579..1779757 (-) 1179 WP_011174228.1 site-specific integrase -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36235.18 Da        Isoelectric Point: 6.8891

>NTDB_id=1037 TT_RS09535 WP_011174226.1 1775457..1776461(-) (dprA) [Thermus thermophilus HB27]
MDPLALALLPGIGPKRLLEVLKAEDPLGFLRERFPEAWRHLPEAEAQAERERRRAEALGVRLLGLWEEGFPEGLKALPQP
PTHLYLKGELPPEREAVALVGTRRASPWALAFARKLARELSEAGLWVVSGLARGIDREAHLGALEAGGRTLGVLGSALDR
VYPPENRPLAQRMDLLSEFPFGTGPKPEFFPRRNRLIAGLCRAVVVVEAPLDSGALITARHALELGKEVLAVPGRPMDER
SLGANRLIQDGAYPVLSAEDVLSYLGMSARPKPLPGLSPEEEALYALLQEKKEALPEALAMALGIPPERVLSLLTLLELK
GLARALPGGRYGPG

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=1037 TT_RS09535 WP_011174226.1 1775457..1776461(-) (dprA) [Thermus thermophilus HB27]
GTGGACCCCTTGGCCCTCGCCCTCCTCCCGGGCATCGGCCCCAAGCGGCTTTTGGAGGTCCTAAAGGCGGAGGACCCTCT
GGGCTTCCTGCGGGAGCGCTTCCCCGAGGCCTGGCGGCACCTCCCCGAGGCCGAGGCCCAGGCGGAAAGGGAGAGGCGGC
GCGCCGAGGCCCTAGGGGTGCGCCTCCTGGGTCTCTGGGAGGAGGGCTTCCCCGAGGGGCTGAAGGCGCTTCCCCAGCCG
CCCACCCACCTCTACCTGAAGGGGGAGCTTCCCCCGGAAAGGGAGGCGGTGGCCCTCGTGGGCACGCGCCGGGCCTCCCC
TTGGGCCCTGGCCTTTGCCCGGAAGCTCGCCCGGGAGCTTTCCGAGGCCGGGCTCTGGGTGGTCTCGGGCCTCGCCCGGG
GCATAGACCGGGAGGCCCACCTCGGGGCCCTCGAGGCCGGGGGGCGCACCCTGGGCGTCTTGGGAAGCGCCCTGGACCGG
GTCTACCCCCCGGAAAACCGCCCTTTGGCCCAACGGATGGACCTCCTCTCCGAGTTCCCCTTCGGCACCGGGCCCAAGCC
CGAGTTCTTTCCCCGGCGGAACCGCCTCATCGCCGGGCTTTGCCGGGCGGTGGTCGTGGTGGAGGCCCCCCTGGACTCCG
GGGCCCTCATCACCGCCCGCCACGCCCTGGAGCTCGGCAAGGAGGTGCTGGCCGTGCCCGGAAGGCCCATGGACGAGCGT
TCTCTGGGAGCCAACCGCCTCATCCAGGACGGGGCCTACCCGGTGCTCTCCGCCGAGGATGTCCTCTCCTATCTGGGAAT
GTCCGCAAGGCCCAAGCCCCTCCCGGGCCTCTCCCCGGAGGAGGAGGCCCTTTACGCCCTCCTGCAGGAAAAGAAGGAGG
CGCTCCCCGAGGCCCTGGCCATGGCCCTGGGGATCCCCCCGGAGCGGGTGCTCTCCCTTCTCACCCTTTTGGAGCTAAAG
GGCCTGGCCCGGGCCCTCCCGGGAGGGCGCTACGGGCCAGGCTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(56-255)

DprA winged helix domain (DprA_WH)

(269-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q72GH5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

38.547

100

0.413
  dprA Vibrio cholerae strain A1552

37.255

100

0.398
  dprA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.392

87.725

0.398

Multiple sequence alignment    



References

[1] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[2] Alexandra Friedrich et al. (2002) Molecular analyses of the natural transformation machinery and identification of pilus structures in the extremely thermophilic bacterium Thermus thermophilus strain HB27. Applied And Environmental Microbiology 68(2):745-55. [PMID: 11823215]