Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   ABD1_RS00865 Genome accession   NC_020547
Coordinates   191417..192547 (-) Length   376 a.a.
NCBI ID   WP_015451369.1    Uniprot ID   -
Organism   Acinetobacter baumannii D1279779     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.


Genomic Context

Location: 186417..197547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD1_RS00840 (ABD1_01610) - 186592..187356 (-) 765 WP_000786954.1 AraC family transcriptional regulator -
  ABD1_RS00845 (ABD1_01620) - 187378..188592 (-) 1215 WP_000372838.1 MFS transporter -
  ABD1_RS00850 (ABD1_01630) - 188729..189379 (+) 651 WP_012300783.1 TetR/AcrR family transcriptional regulator -
  ABD1_RS00855 (ABD1_01640) - 189485..190405 (+) 921 WP_000084546.1 DMT family transporter -
  ABD1_RS00860 (ABD1_01650) - 190797..191366 (-) 570 WP_000633610.1 L-threonylcarbamoyladenylate synthase -
  ABD1_RS00865 (ABD1_01660) dprA 191417..192547 (-) 1131 WP_015451369.1 DNA-processing protein DprA Machinery gene
  ABD1_RS00870 (ABD1_01670) tsaP 192568..193722 (-) 1155 WP_000755276.1 LysM peptidoglycan-binding domain-containing protein Machinery gene
  ABD1_RS00875 (ABD1_01680) def 193845..194375 (+) 531 WP_001196759.1 peptide deformylase -
  ABD1_RS00880 (ABD1_01690) - 194412..194840 (+) 429 WP_000231979.1 DUF2946 family protein -
  ABD1_RS00885 (ABD1_01700) - 194913..197027 (+) 2115 WP_001120689.1 TonB-dependent copper receptor -
  ABD1_RS00890 (ABD1_01710) - 197081..197287 (+) 207 WP_000182542.1 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41973.50 Da        Isoelectric Point: 6.6297

>NTDB_id=1073 ABD1_RS00865 WP_015451369.1 191417..192547(-) (dprA) [Acinetobacter baumannii D1279779]
MLTQLSSHHYHTLKVWYLVQHSLVSFKKIIDYFGNCEKATQPDGLAKWSSLGLHANHLKRVNEFQTTQGQAQFEQLVQQV
RQHTDFILTPDDSGYPTQLLPYTDHPPIIFGKGQAQALLQPQIAIVGSRKPSPHGRQVAYDFAYYLSEKGFFISSGLAYG
IDEAAHQGASAHQRTIAVTGTGLDTTYPAQNKKLAEYILAQNGAIITEFLPGTPPLQQHFPRRNRIVSGLSLGVLVVEAT
LKSGSLITANKAAEQGKTVFAIPGHIYSEFHQGCHQLIREGAILIDHPEQIIEDLALPTQWQSQQQNQTEEANTNTPEIR
EHLIDLYQSLDWVGQNIDQLVVHHNIPVSELTSSLMELELLGLCMQQSGLYLRCRS

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1073 ABD1_RS00865 WP_015451369.1 191417..192547(-) (dprA) [Acinetobacter baumannii D1279779]
ATGCTCACTCAACTTTCTTCACACCACTACCACACACTTAAAGTGTGGTATTTGGTGCAGCACTCTTTAGTCAGCTTTAA
AAAAATTATTGATTATTTTGGAAATTGTGAAAAAGCCACTCAACCTGATGGTTTAGCAAAGTGGTCCTCTCTGGGGCTAC
ATGCTAATCACTTAAAGCGTGTAAACGAATTTCAAACGACCCAAGGTCAGGCGCAGTTTGAACAACTTGTACAACAAGTT
CGTCAGCATACGGATTTTATTTTAACGCCAGATGACTCTGGCTATCCTACTCAATTACTTCCTTATACAGATCACCCACC
TATTATTTTTGGGAAAGGTCAAGCGCAAGCTTTATTGCAACCTCAAATTGCGATTGTCGGTAGCCGAAAACCAAGCCCAC
ATGGACGACAGGTCGCTTATGATTTTGCCTATTACTTAAGTGAAAAGGGCTTTTTCATTAGTAGCGGTCTGGCTTATGGC
ATTGATGAAGCCGCTCATCAAGGTGCATCTGCTCATCAACGTACCATTGCAGTGACGGGTACGGGCTTAGACACTACTTA
TCCAGCTCAAAATAAAAAACTTGCAGAATATATTTTGGCTCAAAACGGCGCCATTATTACTGAGTTTTTACCGGGTACAC
CGCCATTACAACAGCATTTTCCACGTAGAAACCGAATTGTCAGCGGTTTGAGTTTAGGCGTTTTGGTGGTAGAAGCGACA
TTAAAAAGTGGCTCACTCATCACGGCCAATAAAGCTGCTGAACAAGGTAAAACCGTATTCGCGATTCCGGGCCATATTTA
TAGCGAGTTTCATCAAGGATGCCATCAACTTATTCGTGAAGGTGCAATTTTAATCGACCATCCTGAACAGATTATTGAGG
ATCTTGCTTTACCAACCCAATGGCAAAGTCAGCAACAAAATCAAACTGAAGAAGCTAATACGAATACTCCAGAAATTCGT
GAGCATCTGATTGACCTTTATCAGTCTTTAGACTGGGTAGGACAAAATATTGACCAGCTTGTAGTTCACCATAATATTCC
AGTTTCTGAACTGACCTCTTCACTAATGGAACTAGAACTACTCGGTTTATGTATGCAACAATCAGGGCTGTATTTACGTT
GTCGTTCTTAG

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(87-285)

DprA winged helix domain (DprA_WH)

(330-369)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Acinetobacter baumannii strain A118

98.138

100

0.981
  dprA Acinetobacter baylyi ADP1

70.079

100

0.71
  dprA Vibrio cholerae strain A1552

43.505

89.218

0.388

Multiple sequence alignment    



References

[1] Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB2. Frontiers in Microbiology 12:738034. [PMID: 35126321]