Detailed information    

experimental Experimentally validated

Overview

Name   tsaP   Type   Machinery gene
Locus tag   ABD1_RS00870 Genome accession   NC_020547
Coordinates   192568..193722 (-) Length   384 a.a.
NCBI ID   WP_000755276.1    Uniprot ID   -
Organism   Acinetobacter baumannii D1279779     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.


Genomic Context

Location: 187568..198722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD1_RS00850 (ABD1_01630) - 188729..189379 (+) 651 WP_012300783.1 TetR/AcrR family transcriptional regulator -
  ABD1_RS00855 (ABD1_01640) - 189485..190405 (+) 921 WP_000084546.1 DMT family transporter -
  ABD1_RS00860 (ABD1_01650) - 190797..191366 (-) 570 WP_000633610.1 L-threonylcarbamoyladenylate synthase -
  ABD1_RS00865 (ABD1_01660) dprA 191417..192547 (-) 1131 WP_015451369.1 DNA-processing protein DprA Machinery gene
  ABD1_RS00870 (ABD1_01670) tsaP 192568..193722 (-) 1155 WP_000755276.1 LysM peptidoglycan-binding domain-containing protein Machinery gene
  ABD1_RS00875 (ABD1_01680) def 193845..194375 (+) 531 WP_001196759.1 peptide deformylase -
  ABD1_RS00880 (ABD1_01690) - 194412..194840 (+) 429 WP_000231979.1 DUF2946 family protein -
  ABD1_RS00885 (ABD1_01700) - 194913..197027 (+) 2115 WP_001120689.1 TonB-dependent copper receptor -
  ABD1_RS00890 (ABD1_01710) - 197081..197287 (+) 207 WP_000182542.1 hypothetical protein -
  ABD1_RS00895 (ABD1_01720) - 197357..198571 (-) 1215 WP_001177955.1 oxygenase MpaB family protein -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42363.27 Da        Isoelectric Point: 8.5841

>NTDB_id=1053 ABD1_RS00870 WP_000755276.1 192568..193722(-) (tsaP) [Acinetobacter baumannii D1279779]
MKKVFNGMVNFHALGIKKHLLALALCTGVAIGTIAEVHATSPNHNPPSLKSNAPNVYVVKRGDTLWDISGHFLNKPWRWP
EIWASNQHVKNPHWIYPGDRLLLCSLDGRPLVGKDEGDGCVGIIRRYTGQTTHLQPQVRVEALNNSVPVIPLEHIKQWLE
NSTILPADSITNTPYIVGTADQRVLAGKGQTIYARGQGLINGQRYAVYREGEPYYFTDNKGKKHSLGIELLQVASGVAVS
SEKDITTLELTDSYNAEVRRGDRVMPEEQATLPTLFYPVDAKQVTDGGKIIRVMGSIGRAAKNSVVTLDRGTTQGIQVGQ
VFDITQQGESIRDPKTKEVIQLPGQQIGSLMVFRTFDQLSYAYVLESDLPIKVGSSIQSPQFND

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=1053 ABD1_RS00870 WP_000755276.1 192568..193722(-) (tsaP) [Acinetobacter baumannii D1279779]
ATGAAAAAGGTTTTTAACGGCATGGTTAATTTTCATGCCTTGGGGATAAAGAAGCATTTACTTGCCTTGGCGCTCTGTAC
AGGTGTCGCAATCGGTACGATAGCAGAGGTTCATGCAACCAGTCCGAACCATAATCCACCCTCACTTAAAAGTAATGCAC
CCAATGTATATGTTGTAAAACGTGGGGATACTTTATGGGATATTTCAGGACATTTTCTAAATAAACCATGGCGTTGGCCT
GAAATCTGGGCGAGCAACCAACATGTTAAAAATCCACACTGGATCTATCCGGGTGACCGATTACTATTATGTAGCCTAGA
TGGGCGTCCTTTAGTCGGTAAAGATGAGGGCGATGGTTGTGTAGGTATTATTCGCCGCTACACTGGGCAAACCACTCACT
TACAACCTCAAGTTCGCGTCGAAGCATTAAATAATAGTGTTCCGGTTATTCCACTTGAACATATTAAACAGTGGTTAGAG
AACAGTACTATTTTACCAGCCGACTCAATTACCAACACACCTTATATTGTTGGTACAGCCGATCAACGTGTACTTGCAGG
AAAAGGCCAGACTATTTATGCACGAGGACAAGGCTTAATTAATGGGCAGCGTTATGCAGTCTACCGAGAAGGTGAACCTT
ACTATTTCACAGACAATAAAGGTAAAAAGCATAGTTTAGGGATTGAGCTACTACAGGTTGCTTCAGGTGTTGCGGTCTCT
TCTGAAAAAGATATTACTACATTAGAACTAACAGATAGTTATAACGCTGAAGTACGTCGTGGCGATCGTGTTATGCCTGA
AGAACAAGCTACTTTGCCAACATTATTTTACCCAGTAGATGCGAAGCAAGTGACGGATGGTGGCAAGATTATTCGTGTCA
TGGGTTCAATTGGTAGAGCTGCAAAAAATAGTGTAGTGACTTTGGATCGCGGTACAACCCAAGGCATTCAAGTAGGCCAA
GTTTTTGATATTACTCAACAAGGTGAATCAATTCGCGACCCTAAAACCAAAGAAGTAATTCAACTTCCTGGCCAACAAAT
TGGCAGTCTAATGGTTTTTAGAACTTTTGATCAGCTAAGTTATGCTTATGTTTTAGAGAGCGATTTACCAATTAAGGTTG
GTTCAAGCATTCAGTCACCTCAATTTAATGATTAA

Domains

Predicted by InterproScan.

LysM domain (LysM)

(57-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB2. Frontiers in Microbiology 12:738034. [PMID: 35126321]