Detailed information    

experimental Experimentally validated

Overview

Name   pilO   Type   Machinery gene
Locus tag   A1552VC_RS12115 Genome accession   NZ_CP028894
Coordinates   2579534..2580124 (-) Length   196 a.a.
NCBI ID   WP_000157690.1    Uniprot ID   A0A366AJW1
Organism   Vibrio cholerae strain A1552     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

PilO plays a role in type IV-like pilus assembly.


Genomic Context

Location: 2574534..2585124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS12095 (A1552VC_02388) aroB 2575461..2576546 (-) 1086 WP_000439805.1 3-dehydroquinate synthase -
  A1552VC_RS12100 (A1552VC_02389) aroK 2576574..2577098 (-) 525 WP_000818616.1 shikimate kinase AroK -
  A1552VC_RS12105 (A1552VC_02390) pilQ 2577285..2579000 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ family protein Machinery gene
  A1552VC_RS12110 (A1552VC_02391) pilP 2579026..2579544 (-) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  A1552VC_RS12115 (A1552VC_02392) pilO 2579534..2580124 (-) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  A1552VC_RS12120 (A1552VC_02393) pilN 2580117..2580701 (-) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  A1552VC_RS12125 (A1552VC_02394) pilM 2580691..2581707 (-) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  A1552VC_RS12130 (A1552VC_02395) - 2581850..2584327 (+) 2478 WP_000668733.1 penicillin-binding protein 1A -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  tfoX pilO positive effect
  tfoX pilB positive effect
  tfoX pilP positive effect
  tfoX pilA positive effect
  tfoX pilD positive effect
  tfoX dprA positive effect
  tfoX pilM positive effect
  tfoX pilN positive effect
  tfoX comEA positive effect
  hapR comEA positive effect
  crp comEA positive effect
  qstR comEA positive effect
  tfoX pilQ positive effect
  tfoX qstR positive effect
  qstR comEC positive effect
  qstR comF positive effect
  qstR comM positive effect
  crp qstR positive effect
  hapR qstR positive effect
  tfoX comEC positive effect
  hapR comEC positive effect
  tfoX pilC positive effect
  chiS tfoX positive effect
  hapR dns negative effect

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22877.27 Da        Isoelectric Point: 4.4169

>NTDB_id=1173 A1552VC_RS12115 WP_000157690.1 2579534..2580124(-) (pilO) [Vibrio cholerae strain A1552]
MASLQELELDEISEWPLLPQLVVILLIMLLIQGAGYWFYLMPKQDEIALLKQEEETVKATLRIKANKVAVLPQIQAQLDE
LKERYDFLLRQLPVQKELASMLASVNQLGLDSSLTFTRIDWGERESQEFLYRLPLNIELTGSYHDIGDFSQAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=1173 A1552VC_RS12115 WP_000157690.1 2579534..2580124(-) (pilO) [Vibrio cholerae strain A1552]
ATGGCTAGTCTACAAGAGCTAGAACTGGATGAAATTAGTGAATGGCCGCTGCTGCCTCAGTTGGTGGTGATTCTGCTTAT
CATGCTACTGATCCAAGGTGCTGGTTATTGGTTCTATCTCATGCCCAAACAAGACGAGATTGCGCTGTTAAAGCAGGAAG
AGGAAACCGTCAAGGCGACACTGCGCATCAAAGCCAATAAAGTGGCGGTATTGCCGCAGATCCAAGCTCAGTTGGACGAG
CTCAAAGAGCGTTACGATTTTTTACTGCGCCAGCTACCGGTGCAAAAAGAGTTAGCCAGCATGCTGGCGTCAGTCAACCA
GCTTGGGCTCGATAGTTCGCTTACCTTTACGCGGATTGATTGGGGGGAGCGTGAAAGCCAAGAGTTTCTTTACCGCTTAC
CGCTCAATATCGAACTGACGGGGAGCTATCACGATATTGGCGATTTTTCGCAAGCGATTGCCAAGTTACCGCGCATCATC
AACTTCGACGATGTGGACTGGCAGCGTGTCAGCCAAGAGAGTAGCACGCTGCACTTTCGAGTCAGAGCCTACACCTACCA
ATTTAAGCAGGAGGTGGATGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(50-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A366AJW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

71.958

99.474

0.716

Multiple sequence alignment    



References

[1] Patrick Seitz et al. (2013) DNA-uptake machinery of naturally competent Vibrio cholerae. Proceedings of The National Academy of Sciences of The United States of America 110(44):17987-92. [PMID: 24127573]
[2] Mirella Lo Scrudato et al. (2012) The regulatory network of natural competence and transformation of Vibrio cholerae. PLoS Genetics 8(6):e1002778. [PMID: 22737089]