Detailed information    

experimental Experimentally validated

Overview

Name   hapR   Type   Regulator
Locus tag   A1552VC_RS01790 Genome accession   NZ_CP028894
Coordinates   340447..341058 (-) Length   203 a.a.
NCBI ID   WP_000340098.1    Uniprot ID   B2CKP3
Organism   Vibrio cholerae strain A1552     
Function   regulate expression of competence genes   
Competence regulation

Function

HapR is the quorum-sensing regulator that regulate the expression of competence genes.


Genomic Context

Location: 335447..346058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS01760 (A1552VC_00345) - 335971..336540 (-) 570 WP_000836326.1 BON domain-containing protein -
  A1552VC_RS01765 (A1552VC_00346) - 336543..337133 (-) 591 WP_000887295.1 phosphoheptose isomerase -
  A1552VC_RS01770 (A1552VC_00347) - 337137..337505 (-) 369 WP_001893625.1 YraN family protein -
  A1552VC_RS01775 (A1552VC_00348) - 337492..339297 (-) 1806 WP_001015481.1 penicillin-binding protein activator -
  A1552VC_RS01780 (A1552VC_00349) rsmI 339367..340233 (+) 867 WP_000131542.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  A1552VC_RS01790 (A1552VC_00350) hapR 340447..341058 (-) 612 WP_000340098.1 quorum-sensing master transcriptional regulator HapR Regulator
  A1552VC_RS01795 (A1552VC_00351) hpt 341354..341887 (+) 534 WP_000683351.1 hypoxanthine phosphoribosyltransferase -
  A1552VC_RS01800 (A1552VC_00352) can 341962..342630 (-) 669 WP_001114117.1 carbonate dehydratase -
  A1552VC_RS01805 (A1552VC_00353) - 342778..344424 (+) 1647 WP_000484911.1 SulP family inorganic anion transporter -
  A1552VC_RS01810 (A1552VC_00354) - 344808..345725 (+) 918 WP_000271063.1 ABC transporter ATP-binding protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  hapR comEA positive effect
  tfoX comEA positive effect
  tfoX pilO positive effect
  tfoX pilB positive effect
  tfoX pilP positive effect
  tfoX pilA positive effect
  tfoX pilD positive effect
  tfoX dprA positive effect
  tfoX pilM positive effect
  tfoX pilN positive effect
  tfoX pilQ positive effect
  tfoX qstR positive effect
  tfoX comEC positive effect
  tfoX pilC positive effect
  chiS tfoX positive effect
  hapR qstR positive effect
  hapR dns negative effect
  hapR comEC positive effect
  crp comEA positive effect
  crp qstR positive effect
  qstR comEA positive effect
  qstR comEC positive effect
  qstR comF positive effect
  qstR comM positive effect

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23692.39 Da        Isoelectric Point: 6.6356

>NTDB_id=1147 A1552VC_RS01790 WP_000340098.1 340447..341058(-) (hapR) [Vibrio cholerae strain A1552]
MDASIEKRPRTRLSPQKRKLQLMEIALEVFAKRGIGRGGHADIAEIAQVSVATVFNYFPTREDLVDDVLNFVVRQYSNFL
TDHIDLDLDVKTNLQTLCKEMVKLAMTDCHWLKVWFEWSASTRDEVWPLFVSTNRTNQLLIRNMFMKAMERGELCEKHDV
DNMASLFHGIFYSIFLQVNRLGEQEAVYKLADSYLNMLCIYKN

Nucleotide


Download         Length: 612 bp        

>NTDB_id=1147 A1552VC_RS01790 WP_000340098.1 340447..341058(-) (hapR) [Vibrio cholerae strain A1552]
ATGGACGCATCAATCGAAAAACGCCCTCGAACTCGGCTATCGCCTCAAAAACGCAAACTACAACTGATGGAAATCGCGTT
GGAAGTGTTTGCTAAACGCGGCATTGGTCGTGGTGGTCACGCAGATATTGCCGAGATTGCGCAAGTCTCCGTTGCAACAG
TGTTCAACTACTTCCCAACTCGTGAAGACTTGGTTGACGATGTGCTGAATTTTGTGGTTCGTCAGTACTCCAACTTCTTG
ACCGATCACATCGATCTTGATTTGGATGTGAAAACCAACCTACAAACTCTGTGCAAAGAGATGGTGAAATTGGCGATGAC
CGATTGTCACTGGCTCAAAGTCTGGTTTGAGTGGAGTGCTTCAACCCGTGACGAAGTTTGGCCACTGTTTGTTTCCACCA
ACCGAACTAACCAACTGCTGATCAGAAACATGTTTATGAAAGCGATGGAGCGTGGCGAATTGTGTGAGAAACACGATGTC
GATAACATGGCCAGCCTGTTCCACGGCATCTTCTACTCCATCTTCTTACAAGTGAACCGTTTAGGTGAACAAGAAGCAGT
GTATAAGTTGGCCGATAGCTACCTCAATATGCTGTGTATCTATAAGAACTAG

Domains

Predicted by InterproScan.

Bacterial regulatory proteins, tetR family (TetR_N)

(23-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 6D7R
  PDB 7XXN
  PDB 7XXO
  PDB 7XXS
  PDB 7XXT
  PDB 7XY0
  PDB 7XY5
  PDB 7XYI
  PDB 7Y4J

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hapR Vibrio cholerae C6706

100

100

1
  opaR Vibrio parahaemolyticus RIMD 2210633

72.222

97.537

0.704

Multiple sequence alignment    



References

[1] Sandrine Stutzmann et al. (2020) Comparison of chitin-induced natural transformation in pandemic Vibrio cholerae O1 El Tor strains. Environmental Microbiology 22(10):4149-4166. [PMID: 32860313]
[2] Mirella Lo Scrudato et al. (2013) A transcriptional regulator linking quorum sensing and chitin induction to render Vibrio cholerae naturally transformable. Nucleic Acids Research 41(6):3644-58. [PMID: 23382174]
[3] Mirella Lo Scrudato et al. (2012) The regulatory network of natural competence and transformation of Vibrio cholerae. PLoS Genetics 8(6):e1002778. [PMID: 22737089]
[4] Gaia Suckow et al. (2011) Quorum sensing contributes to natural transformation of Vibrio cholerae in a species-specific manner. Journal of Bacteriology 193(18):4914-24. [PMID: 21784943]
[5] Karin L Meibom et al. (2005) Chitin induces natural competence in Vibrio cholerae. Science (New York, N.Y.) 310(5755):1824-7. [PMID: 16357262]