Detailed information    

experimental Experimentally validated

Overview

Name   comEC   Type   Machinery gene
Locus tag   A1552VC_RS08405 Genome accession   NZ_CP028894
Coordinates   1799767..1802010 (-) Length   747 a.a.
NCBI ID   WP_000173776.1    Uniprot ID   -
Organism   Vibrio cholerae strain A1552     
Function   ssDNA transport through the inner membrane   
DNA binding and uptake

Function

ComEA shuttles the transforming DNA through the periplasm and toward the inner membrane aqueous channel ComEC, which, potentially in concert with ComF, transports a single DNA strand into the cytoplasm.


Genomic Context

Location: 1794767..1807010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS08385 (A1552VC_01655) kdsB 1796060..1796818 (-) 759 WP_000011329.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  A1552VC_RS08390 (A1552VC_01656) - 1796818..1796997 (-) 180 WP_000350068.1 Trm112 family protein -
  A1552VC_RS08395 (A1552VC_01657) lpxK 1796978..1797985 (-) 1008 WP_001994134.1 tetraacyldisaccharide 4'-kinase -
  A1552VC_RS08400 (A1552VC_01658) msbA 1797988..1799736 (-) 1749 WP_000052153.1 lipid A ABC transporter ATP-binding protein/permease MsbA -
  A1552VC_RS08405 (A1552VC_01659) comEC 1799767..1802010 (-) 2244 WP_000173776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  A1552VC_RS08410 (A1552VC_01660) - 1802019..1802549 (+) 531 WP_001881633.1 DUF2062 domain-containing protein -
  A1552VC_RS08415 (A1552VC_01661) lolE 1802664..1803908 (-) 1245 WP_000493010.1 lipoprotein-releasing ABC transporter permease subunit LolE -
  A1552VC_RS08420 (A1552VC_01662) lolD 1803909..1804595 (-) 687 WP_001061290.1 lipoprotein-releasing ABC transporter ATP-binding protein LolD -
  A1552VC_RS08425 (A1552VC_01663) lolC 1804588..1805796 (-) 1209 WP_000468900.1 lipoprotein-releasing ABC transporter permease subunit LolC -
  A1552VC_RS08430 (A1552VC_01664) - 1805972..1806547 (+) 576 WP_000999601.1 PilZ domain-containing protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  qstR comEC positive effect
  tfoX comEC positive effect
  hapR comEC positive effect
  qstR comF positive effect
  qstR comEA positive effect
  tfoX comEA positive effect
  hapR comEA positive effect
  crp comEA positive effect
  qstR comM positive effect
  crp qstR positive effect
  tfoX qstR positive effect
  tfoX pilO positive effect
  tfoX pilB positive effect
  tfoX pilP positive effect
  tfoX pilA positive effect
  tfoX pilD positive effect
  tfoX dprA positive effect
  tfoX pilM positive effect
  tfoX pilN positive effect
  tfoX pilQ positive effect
  tfoX pilC positive effect
  chiS tfoX positive effect
  hapR qstR positive effect
  hapR dns negative effect

Sequence

Protein


Download         Length: 747 a.a.        Molecular weight: 84511.71 Da        Isoelectric Point: 7.7612

>NTDB_id=1149 A1552VC_RS08405 WP_000173776.1 1799767..1802010(-) (comEC) [Vibrio cholerae strain A1552]
MTLLSNYWSLISFSITVLSAPYWPWMPSWGWAWLCLIVMVLLGYHRVGRQFLGFVAAILTIVLQGNLIRDQSNVLYQAGP
DIIIKGRVDSFFTQTRYAYEGFVLIHEVNGQTLNKMTRPRIRLSAPLLLQPNDRVEFSVTLKPIVGRLNQTGFDLEAHYM
AQSVVARAVVKPDTAYQIVQESGIRSSLFFELEQLTHTSPYQGLILALTFGERKGIDEQEWQALRNSGLIHLVAISGLHI
GIAFSVGYFLGLGMMRFHAQLLWSPFVCGALLAVLYAWLAGFTLPTQRALIMCLLNVALIMLAFPLSALKRILLTLVAVL
LWSPFASLSNSFWMSFLAVAIVLYQLASQSQRQVWWKALLWAQVFLVCLMAPVTAYFFGGLSVTAVLYNLVFIPWFSLVI
VPALFLGLLLMVVWPSVAAAYWPWVDWTFLPLDWALQFADVGWWVVPSKVQGVVAASVAILLLYRFMSLKACSLLLGMIG
LWWWFPSLTPLWRMDVLDVGHGLAIVIEQDERAIVYDTGSSWPGGSYVQSVIEPMLQQRGLRQVDGVILSHLDNDHAGDW
QGLAERWQPNWIRASQLGTEFMPCIRGESWQWQSLHFTVLWPPQAVSRAYNQHSCVIRMTDTQSNHSVLLSGDVTAMGEW
LLARDGAQLQSEVMIVPHHGSKTSSTAEFIAQVNPKLAIASVAKDNRWNLPNPQVVARYQAQQVEWLDTGHAGQISLFFY
LDQLDWFTQRSLGWQPWYRQMLRKGVE

Nucleotide


Download         Length: 2244 bp        

>NTDB_id=1149 A1552VC_RS08405 WP_000173776.1 1799767..1802010(-) (comEC) [Vibrio cholerae strain A1552]
ATGACTCTCTTGTCGAATTACTGGTCTCTCATTTCTTTTTCGATCACCGTGCTGTCTGCCCCTTATTGGCCTTGGATGCC
GAGTTGGGGTTGGGCTTGGTTATGCCTTATTGTTATGGTTTTGCTCGGTTATCACCGAGTTGGCCGTCAATTCCTTGGCT
TCGTGGCTGCCATACTAACCATTGTGCTACAGGGCAACCTTATACGAGATCAATCCAATGTGCTCTATCAAGCAGGGCCG
GATATTATCATAAAAGGCCGTGTTGACAGCTTTTTTACGCAAACTCGTTACGCTTATGAGGGTTTTGTCCTCATTCATGA
AGTGAATGGACAAACCTTAAACAAAATGACTCGCCCTCGCATACGTTTAAGTGCCCCTTTACTGTTACAACCCAATGATC
GCGTCGAATTTTCGGTAACTCTCAAGCCGATAGTGGGTCGACTCAACCAAACCGGCTTTGATTTAGAAGCGCATTACATG
GCGCAATCTGTCGTCGCACGAGCGGTCGTAAAACCTGACACTGCTTATCAAATTGTGCAAGAGAGTGGCATAAGGTCAAG
TTTGTTTTTTGAGCTAGAGCAATTAACGCATACCAGCCCATACCAAGGATTGATCTTAGCCCTGACGTTTGGCGAGCGAA
AAGGTATTGATGAGCAAGAGTGGCAAGCCTTACGCAATAGTGGCTTAATTCATTTAGTGGCCATTTCGGGGCTGCACATT
GGTATCGCTTTTAGCGTGGGGTATTTTCTCGGGCTCGGCATGATGCGTTTTCATGCTCAGTTATTGTGGTCCCCTTTTGT
GTGTGGGGCTTTACTGGCGGTGCTCTACGCTTGGCTGGCCGGATTTACGTTGCCTACTCAGCGTGCATTGATTATGTGCT
TACTCAATGTGGCGTTGATCATGTTGGCTTTTCCTCTTTCCGCGCTCAAGCGGATTCTACTCACCTTAGTCGCGGTCTTG
CTTTGGTCGCCATTCGCCTCACTTTCAAACAGTTTCTGGATGTCGTTTTTGGCGGTCGCGATTGTTCTCTACCAATTAGC
CAGTCAAAGCCAGCGTCAGGTGTGGTGGAAAGCTCTTCTTTGGGCGCAGGTGTTCCTCGTCTGTTTAATGGCACCGGTCA
CGGCCTATTTTTTCGGTGGCTTAAGCGTAACGGCAGTTCTGTACAATTTGGTGTTTATTCCTTGGTTTTCGTTGGTGATT
GTCCCAGCTTTGTTTTTGGGTCTATTACTCATGGTGGTATGGCCTAGTGTGGCCGCCGCTTACTGGCCTTGGGTGGATTG
GACGTTTTTACCGCTCGATTGGGCTTTGCAGTTTGCCGATGTAGGCTGGTGGGTGGTCCCCAGCAAAGTACAAGGTGTGG
TCGCAGCGAGTGTGGCCATCCTCTTGCTTTATCGATTTATGAGCCTAAAAGCCTGCAGCTTATTATTGGGTATGATTGGC
TTATGGTGGTGGTTTCCCTCTCTCACTCCACTTTGGCGAATGGATGTGCTGGATGTTGGACATGGCTTGGCGATTGTGAT
TGAGCAAGATGAGCGAGCAATTGTCTACGATACAGGCAGCAGTTGGCCGGGAGGCAGCTATGTGCAAAGCGTGATTGAGC
CTATGCTCCAACAGCGGGGGCTACGCCAAGTGGATGGAGTGATTTTAAGTCATCTTGATAATGATCATGCGGGTGATTGG
CAAGGTTTAGCTGAGCGCTGGCAACCCAATTGGATTCGTGCCAGCCAACTCGGGACAGAGTTTATGCCTTGTATCCGTGG
TGAAAGCTGGCAGTGGCAATCTCTCCATTTTACGGTGTTATGGCCACCACAAGCGGTTAGCCGAGCGTACAACCAGCATT
CGTGTGTGATTCGTATGACCGATACTCAGTCTAACCATTCTGTACTGCTCTCCGGGGATGTCACAGCCATGGGGGAGTGG
CTGCTTGCTCGCGACGGAGCGCAACTGCAAAGTGAGGTGATGATCGTGCCGCACCACGGCAGTAAAACATCGTCCACCGC
AGAGTTTATTGCCCAAGTGAATCCCAAACTTGCGATTGCTTCTGTGGCGAAAGATAACCGCTGGAATTTGCCTAATCCGC
AAGTCGTGGCACGTTATCAAGCTCAGCAAGTTGAGTGGCTAGATACTGGACACGCTGGGCAAATTAGCCTCTTTTTCTAT
CTAGATCAGCTGGATTGGTTTACCCAGCGTAGCCTTGGCTGGCAGCCTTGGTATAGGCAGATGCTGCGTAAAGGAGTAGA
ATGA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(208-464)

Domain of unknown function (DUF4131) (DUF4131)

(21-173)

Metallo-beta-lactamase superfamily (Lactamase_B)

(499-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Vibrio parahaemolyticus RIMD 2210633

41.215

100

0.418
  comEC Vibrio campbellii strain DS40M4

40.957

100

0.412

Multiple sequence alignment    



References

[1] Patrick Seitz et al. (2014) DNA transport across the outer and inner membranes of naturally transformable Vibrio cholerae is spatially but not temporally coupled. MBio 5(4):e01409-14. [PMID: 25139903]
[2] Patrick Seitz et al. (2013) DNA-uptake machinery of naturally competent Vibrio cholerae. Proceedings of The National Academy of Sciences of The United States of America 110(44):17987-92. [PMID: 24127573]
[3] Gaia Suckow et al. (2011) Quorum sensing contributes to natural transformation of Vibrio cholerae in a species-specific manner. Journal of Bacteriology 193(18):4914-24. [PMID: 21784943]