Detailed information NTG0001143

Experimentally validated

Overview

• Name: chiS
• Type: Regulator
• Locus tag: A1552VC_RS01965
• Genome accession: NZ_CP028894
• Coordinates: 386112..389645 (-)
• Length: 1177 a.a.
• NCBI ID: WP_001883436.1
• Uniprot ID: -
• Organism: Vibrio cholerae strain A1552
• Function: chitin sensor Competence regulation

Function

The presence of chitin is sensed by the chitin sensor ChiS, due to chitinase-released GlcNAc di-/oligomers, and the signal is then transferred via TfoR towards the production of TfoX.

Genomic Context

Location: 381112..394645

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A1552VC_RS01945 (A1552VC_00381)	-	381347..382372 (-)	1026	WP_000646270.1	ABC transporter permease	-
A1552VC_RS01950 (A1552VC_00382)	-	382375..383361 (-)	987	WP_000540337.1	ABC transporter permease	-
A1552VC_RS01955 (A1552VC_00383)	-	383531..385201 (-)	1671	WP_000880698.1	ABC transporter substrate-binding protein	-
A1552VC_RS01965 (A1552VC_00384)	chiS	386112..389645 (-)	3534	WP_001883436.1	two-component system sensor histidine kinase ChiS	Regulator
A1552VC_RS01970 (A1552VC_00385)	rsmC	389709..390731 (-)	1023	WP_001183380.1	16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC	-
A1552VC_RS01975 (A1552VC_00386)	-	390804..391889 (-)	1086	WP_001290813.1	AI-2E family transporter	-
A1552VC_RS01980 (A1552VC_00388)	hemL	392143..393441 (-)	1299	WP_000167257.1	glutamate-1-semialdehyde 2,1-aminomutase	-
A1552VC_RS01985 (A1552VC_00389)	erpA	393725..394066 (+)	342	WP_000005741.1	iron-sulfur cluster insertion protein ErpA	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
chiS	tfoX	positive effect
tfoX	pilO	positive effect
tfoX	pilB	positive effect
tfoX	pilP	positive effect
tfoX	pilA	positive effect
tfoX	pilD	positive effect
tfoX	dprA	positive effect
tfoX	pilM	positive effect
tfoX	pilN	positive effect
tfoX	comEA	positive effect
hapR	comEA	positive effect
crp	comEA	positive effect
qstR	comEA	positive effect
tfoX	pilQ	positive effect
tfoX	qstR	positive effect
qstR	comEC	positive effect
qstR	comF	positive effect
qstR	comM	positive effect
crp	qstR	positive effect
hapR	qstR	positive effect
tfoX	comEC	positive effect
hapR	comEC	positive effect
tfoX	pilC	positive effect
hapR	dns	negative effect

Sequence

Protein

• Length: 1177 a.a.
• Molecular weight: 133134.30 Da
• Isoelectric Point: 6.8941

>NTDB_id=1143 A1552VC_RS01965 WP_001883436.1 386112..389645(-) (chiS) [Vibrio cholerae strain A1552]
MRLCPRFSLDLLVKVKKLVNFVTLGFSCLSPTHAWRSPQSFTESNTLIMFRFYRKQKFKRLQNTLMAAFLALSIIPLTIT
ALFFLHSHSKDLEQQSTSYLVSVRDNKQQQVIDYMMAKESEVMGFVRSELAYASGGRFYGLVNAFQRLDVSIEAAREHAQ
QRYITGSGDQIKTSVLPQSSSYVGSERYRLLHKRYHWAYLELLKRSDFDDILLVDIDGNVVYSIYKYDNFGTNLLTGKYQ
DTNLGHTFKRLEQTVNEQRKTNEDFTPVVISDFVQEDGKQYAWLGAPIIQQGYLHSYAMFRLPSNAITKLIAEGSSNPSM
QTILVGQDHRSRTLTSAEIAVEKSKAVVDLALSGKRAVGTYTNTDGEQIIAAYAPINLKNIHWALVVELPEKEAFARVRQ
LEKLFVFAMLTAIVLVVIASHYLSNFITSPLLKLTWAAERVSAGDLDEAMINTERKDEIGRLAVSFERMQRSIREKISLI
KSQNKELESNLLIIRKQNDELQLANKLKDEFLATTSHELRTPLHGMIGIAEALISGANGPISAAHKYQLDIIISSGQRLA
TLVDDLLDYHKMRYGALDIQRCAVDLSSATRLVLELSHHLLGKKTLRIINQVSEQPVWVSADPQRLEQVLYNLIGNAIKY
TSEGKIVISATYIDDKVRVQVVDTGQGIPAEQLEHIFEPLIQAGHDASRYRQGAGLGLSISRQLIELMHGTLYVSSQPMV
GTTFSFTLPLASEEEIAQARLTELPHFQAPEVLDSELPEQSNLPENEHGPLLLVADDEPVNLRVLDSFLRLEGYRVHTAQ
DGHQVLEAIKREKPELVLLDIMMPGMSGYQVCEKLRQTYDHAELPIIMLTALNQSDDRVRGFEAGANDYLSKPFNKQELA
ARIVAHLTASKAELRRIENAQLQKELKHRAMVEASLLETQGRLLEQLESAPEAILCVKEGNKVRFANEAAARLFRRTPEQ
LKRSNAEELIAPKFLNIEQEHYCGNIDVYVDDVRQHLSADVLRLPQGSGLQAMYIFNVGGSVNAARIHNLETAVEALSSY
AFEGDKDKLQKLKELGGEFTRLADKASGEYQSKQDLMRAVLVEAMTSALNYWERVSGQSKFTFAEQSGLWRVYLDRSTLQ
TRTLDKYLRIETLPKTPRWRTVLNSLDYILEHCKEAGPERTHIEMQRDKLQKLLTSE

Nucleotide

• Length: 3534 bp

>NTDB_id=1143 A1552VC_RS01965 WP_001883436.1 386112..389645(-) (chiS) [Vibrio cholerae strain A1552]
TTGCGTCTATGCCCACGCTTTAGCTTGGACTTACTTGTCAAAGTAAAAAAACTCGTTAATTTCGTTACTTTAGGTTTTTC
TTGTCTATCACCCACTCATGCTTGGCGATCGCCGCAGAGTTTCACCGAAAGTAACACCTTAATTATGTTTAGGTTCTATC
GAAAACAAAAATTCAAACGCCTGCAAAATACCTTGATGGCTGCGTTTTTGGCCCTCAGCATTATTCCTTTGACCATTACT
GCGTTGTTTTTCCTACATTCACACAGTAAAGATCTGGAGCAGCAGAGCACTTCTTACCTCGTTTCTGTTCGGGATAATAA
ACAGCAACAGGTCATCGACTACATGATGGCCAAAGAGTCGGAAGTGATGGGTTTTGTTCGCTCCGAGCTGGCTTATGCCA
GTGGCGGGCGTTTTTATGGTCTGGTGAATGCTTTCCAGCGTTTAGACGTCAGTATTGAAGCGGCTCGTGAACATGCTCAG
CAGCGCTATATCACCGGATCGGGTGACCAAATAAAAACCTCGGTGCTCCCCCAATCAAGCAGCTACGTGGGCAGTGAACG
CTATCGACTGCTGCATAAACGCTACCACTGGGCTTACTTAGAACTGCTGAAACGGTCGGACTTTGATGACATTTTATTGG
TCGATATTGATGGCAACGTCGTTTATTCGATTTATAAATACGACAATTTTGGCACCAACTTATTAACGGGCAAATACCAA
GACACCAATCTAGGCCATACCTTCAAGCGTTTAGAACAAACGGTCAATGAACAGCGCAAAACCAACGAAGATTTCACCCC
TGTCGTGATTTCTGATTTCGTACAAGAGGATGGTAAACAATACGCTTGGCTTGGCGCACCGATTATTCAGCAAGGCTACT
TGCATAGTTACGCGATGTTCCGCTTACCAAGCAATGCGATCACCAAACTCATCGCAGAAGGCAGTAGCAATCCTTCGATG
CAAACCATTCTAGTGGGACAAGATCACCGCTCAAGAACCTTAACTTCCGCCGAAATTGCGGTTGAAAAAAGTAAAGCTGT
GGTCGATTTAGCCTTATCGGGCAAACGCGCCGTAGGCACTTACACCAATACCGATGGTGAACAGATCATCGCGGCCTACG
CACCGATCAATTTGAAAAATATCCATTGGGCTCTGGTGGTTGAACTGCCAGAGAAAGAAGCGTTTGCTCGCGTTCGTCAG
TTAGAGAAACTGTTTGTTTTTGCGATGTTGACGGCTATCGTCTTAGTGGTCATCGCCTCGCATTACCTTTCCAATTTCAT
TACCTCACCGCTTTTGAAGCTGACATGGGCAGCCGAACGTGTTTCGGCAGGCGATCTTGATGAAGCCATGATCAATACCG
AACGCAAAGATGAAATTGGCCGCTTGGCGGTCAGTTTTGAGCGTATGCAGCGTTCCATTCGCGAAAAAATATCGCTGATC
AAATCGCAAAATAAAGAGTTAGAGAGCAACTTACTGATCATTCGTAAGCAAAACGATGAGTTACAGCTAGCGAATAAGCT
AAAAGATGAGTTCCTCGCGACCACATCACATGAATTACGCACCCCATTGCATGGCATGATTGGTATTGCCGAAGCCCTGA
TCTCGGGGGCAAATGGCCCGATTTCAGCAGCGCACAAGTATCAACTCGACATCATTATCAGCAGTGGGCAGCGCTTGGCG
ACTCTGGTTGATGATCTACTCGATTACCACAAAATGCGCTATGGCGCGCTGGATATTCAAAGGTGTGCCGTCGATTTATC
GAGCGCTACGCGCTTAGTCTTGGAGCTTTCTCACCATTTACTTGGCAAGAAAACGCTGCGCATTATTAATCAAGTCTCAG
AACAACCGGTCTGGGTTTCTGCGGATCCTCAACGCTTAGAGCAAGTCCTGTATAACTTGATCGGCAATGCCATTAAGTAC
ACTTCCGAAGGCAAAATTGTTATCTCCGCAACCTATATTGACGATAAAGTGCGCGTGCAGGTGGTGGATACAGGTCAAGG
GATTCCGGCAGAACAACTGGAGCACATCTTTGAGCCATTGATTCAAGCGGGCCATGATGCGAGTCGCTATCGCCAAGGCG
CAGGACTCGGGCTTTCCATCAGTCGTCAGTTAATCGAATTGATGCACGGCACTCTCTACGTAAGTAGTCAGCCGATGGTT
GGCACTACCTTTAGCTTCACGCTGCCTCTGGCGAGCGAAGAAGAGATTGCTCAAGCGCGCTTGACCGAACTCCCCCACTT
CCAAGCACCGGAAGTGTTGGATTCTGAACTGCCCGAGCAAAGCAACTTACCCGAAAATGAACATGGCCCACTTCTGTTGG
TCGCCGATGATGAACCAGTCAACCTGCGTGTGCTGGACAGCTTTTTACGTCTTGAAGGTTATCGCGTACATACCGCGCAA
GATGGCCATCAAGTACTTGAAGCAATTAAACGTGAAAAACCTGAATTGGTGTTACTCGACATCATGATGCCAGGCATGAG
CGGTTATCAAGTGTGTGAAAAACTTCGACAAACTTACGACCACGCTGAACTGCCTATTATTATGCTGACGGCTCTCAACC
AGTCTGATGACCGGGTACGAGGGTTTGAAGCTGGAGCCAATGACTACTTATCCAAACCATTCAACAAACAAGAGCTGGCC
GCGCGCATTGTCGCTCACCTCACGGCGAGCAAAGCCGAACTGCGCCGCATTGAGAATGCCCAACTGCAAAAAGAGTTGAA
ACATCGCGCTATGGTAGAAGCGAGCCTATTGGAAACCCAAGGCCGATTATTGGAACAGTTAGAATCTGCGCCAGAAGCGA
TTTTGTGCGTTAAAGAGGGCAATAAAGTACGTTTTGCTAACGAAGCGGCAGCACGTTTATTTAGACGTACCCCTGAGCAG
CTCAAACGCTCCAACGCCGAAGAACTGATTGCTCCTAAATTCTTGAATATTGAGCAAGAGCATTACTGCGGCAATATTGA
TGTTTACGTAGATGATGTGCGCCAGCACCTCTCTGCCGATGTCCTTCGCTTGCCGCAAGGTTCTGGCTTACAAGCGATGT
ACATTTTTAATGTCGGCGGCAGCGTCAATGCCGCGCGGATTCATAACCTCGAAACCGCGGTAGAAGCCCTGTCTAGCTAT
GCCTTCGAAGGCGATAAAGACAAGCTGCAAAAACTCAAGGAACTGGGTGGTGAGTTTACTCGTCTTGCAGATAAAGCCTC
GGGCGAGTATCAATCGAAACAAGATTTGATGCGTGCCGTGTTAGTCGAAGCCATGACCAGTGCCTTGAACTATTGGGAAC
GAGTCTCAGGGCAAAGCAAGTTCACGTTTGCCGAACAAAGTGGCTTGTGGCGCGTTTATCTTGACCGCAGCACCCTACAA
ACTCGTACCCTAGACAAATACTTACGAATTGAGACACTGCCTAAAACACCGCGTTGGCGAACCGTACTGAACTCGCTCGA
CTACATTCTTGAGCATTGCAAAGAAGCAGGCCCTGAACGCACTCACATCGAAATGCAGCGCGATAAATTGCAAAAACTCC
TGACCAGTGAATAA

Domains

Predicted by InterproScan.

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (622-731)

• Response regulator receiver domain (Response_reg) (773-883)

• His Kinase A (phospho-acceptor) domain (HisKA) (508-573)

• PAS domain (PAS_8) (927-961)

• HAMP domain (HAMP) (423-475)

References

• [1]: Allison C Hullinger et al. (2025) Two transmembrane transcriptional regulators coordinate to activate chitin-induced natural transformation in Vibrio cholerae. PLoS Genetics 21(2):e1011606. [PMID: 39965000]
• [2]: Mirella Lo Scrudato et al. (2012) The regulatory network of natural competence and transformation of Vibrio cholerae. PLoS Genetics 8(6):e1002778. [PMID: 22737089]