Detailed information NTG0466564

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H0N27_RS13365
• Genome accession: NZ_CP059039
• Coordinates: 2871739..2872857 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain A118
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2866739..2877857

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H0N27_RS13355 (H0N27_13355)	-	2869855..2870547 (-)	693	WP_168726751.1	YggS family pyridoxal phosphate-dependent enzyme	-
H0N27_RS13360 (H0N27_13360)	pilT	2870674..2871711 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
H0N27_RS13365 (H0N27_13365)	pilU	2871739..2872857 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H0N27_RS13370 (H0N27_13370)	fur	2872927..2873364 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
H0N27_RS13375 (H0N27_13375)	-	2873476..2873874 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
H0N27_RS13380 (H0N27_13380)	-	2873929..2874252 (-)	324	WP_045901052.1	RnfH family protein	-
H0N27_RS13385 (H0N27_13385)	-	2874252..2875319 (-)	1068	WP_168726750.1	hypothetical protein	-
H0N27_RS13390 (H0N27_13390)	-	2875472..2875927 (-)	456	WP_000782976.1	bacteriohemerythrin	-
H0N27_RS13395 (H0N27_13395)	-	2876180..2877028 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=466564 H0N27_RS13365 WP_000347034.1 2871739..2872857(+) (pilU) [Acinetobacter baumannii strain A118]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=466564 H0N27_RS13365 WP_000347034.1 2871739..2872857(+) (pilU) [Acinetobacter baumannii strain A118]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAATGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCACAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39