Detailed information    

experimental Experimentally validated

Overview

Name   cqsS   Type   Regulator
Locus tag   A1552VC_RS17020 Genome accession   NZ_CP028895
Coordinates   455148..457208 (+) Length   686 a.a.
NCBI ID   WP_001882375.1    Uniprot ID   Q9KM66
Organism   Vibrio cholerae strain A1552     
Function   autoinducer sensor   
Competence regulation

Function

Extracellular CAI‑1 is sensed by the sensor kinase CqsS, whereas AI‑2 is sensed by the LuxPQ receptor complex. at high cell densities both signals promote dephosphorylation of LuxO, thereby promoting HapR production.


Genomic Context

Location: 450148..462208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS17010 (A1552VC_A03237) - 450869..452008 (+) 1140 WP_000670244.1 exonuclease SbcCD subunit D -
  A1552VC_RS17015 (A1552VC_A03238) - 452010..455051 (+) 3042 WP_001247622.1 AAA family ATPase -
  A1552VC_RS17020 (A1552VC_A03239) cqsS 455148..457208 (+) 2061 WP_001882375.1 quorum-sensing CAI-1 autoinducer sensor kinase/phosphatase CqsS Regulator
  A1552VC_RS17030 (A1552VC_A03240) cqsA 457437..458606 (-) 1170 WP_001039912.1 alpha-hydroxyketone-type quorum-sensing autoinducer synthase Regulator
  A1552VC_RS17035 (A1552VC_A03241) - 458803..459765 (-) 963 WP_001104009.1 TDT family transporter -
  A1552VC_RS17040 (A1552VC_A03242) clcA 459912..461318 (-) 1407 WP_000107451.1 H(+)/Cl(-) exchange transporter ClcA -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  cqsS luxO positive effect
  luxO hapR positive effect
  cqsA cqsS positive effect
  luxQ luxO positive effect
  luxS luxQ positive effect
  luxP luxO positive effect
  luxS luxP positive effect

Sequence

Protein


Download         Length: 686 a.a.        Molecular weight: 78382.17 Da        Isoelectric Point: 5.3891

>NTDB_id=1162 A1552VC_RS17020 WP_001882375.1 455148..457208(+) (cqsS) [Vibrio cholerae strain A1552]
MIVSMDVIKRVYQYAEPNLSLVGWMGMLGFPAYYFIWEYWFPQSYENLGLRCAAAVLFGGLVFRDSMPKKWQRYMPGYFL
FTIGFCLPFFFAFMMLMNDWSTIWAMSFMASIFLHILLVHDTRVMALQALFSVLVAYLAVYGLTDFHPTTLIEWQYIPIF
LFTYVFGNLCFFRNQISHETKVSIAKTFGAGIAHEMRNPLSALKTSIDVVRTMIPKPQTAAHTDYSLDAQELDLLHQILN
EADDVIYSGNNAIDLLLTSIDENRVSPASFKKHSVVDVIEKAVKTFPYKNAADQHSVELEVHQPFDFFGSDTLLTYALFN
LLKNAFYYQKEHFSVCISIEQTSEHNLIRVRDNGVGIAPEMLEDIFRDFYTFGKNGSYGLGLPFCRKVMSAFGGTIRCAS
QQGQWTEFVLSFPRYDSDTVNEIKTELLKTKSLIYIGSNQAIVRELNQLAVEDEFGFTAISAQQAVRRQDYEFEFDLILL
DLDDATAQGELLPKLEGTLSFAEGCIGYVYDPGKTYAVNINRYLRIQPISIHSILRKPRKIIERLLFEQESLSMNRNVIP
LQKSRHERRILVVDDNQSIRTFTAILLEQQGYEVVQANDGSEVLKHMESQNIDLVLMDIEMPNVGGLEATRLIRNSEHEY
KNIPIIGYTGDNSPKTLALVQTSGMNDFIVKPADRDVLLNKVAAWV

Nucleotide


Download         Length: 2061 bp        

>NTDB_id=1162 A1552VC_RS17020 WP_001882375.1 455148..457208(+) (cqsS) [Vibrio cholerae strain A1552]
GTGATAGTGAGCATGGATGTAATAAAGAGAGTGTATCAATACGCAGAACCTAATCTGTCCTTAGTCGGATGGATGGGCAT
GCTTGGCTTTCCTGCCTACTACTTCATCTGGGAATATTGGTTTCCGCAATCTTACGAAAATTTAGGGCTGCGTTGTGCGG
CAGCGGTGCTGTTCGGTGGGCTGGTGTTTCGTGATTCGATGCCTAAGAAATGGCAGCGGTATATGCCGGGCTATTTTTTA
TTCACCATAGGTTTCTGCTTACCCTTTTTCTTCGCTTTTATGATGTTAATGAATGACTGGTCGACAATTTGGGCCATGTC
GTTTATGGCGTCCATTTTTCTGCATATTCTCTTAGTACACGATACGAGAGTGATGGCGTTACAAGCACTTTTCTCTGTGT
TGGTGGCCTATCTTGCGGTATATGGTTTGACGGATTTTCACCCGACGACTTTGATTGAATGGCAATACATACCGATCTTT
TTGTTTACTTATGTATTTGGGAATTTATGTTTTTTCCGTAACCAAATCTCTCATGAGACCAAAGTGTCGATTGCCAAAAC
GTTCGGAGCGGGGATTGCCCATGAAATGCGTAATCCACTCAGCGCTTTGAAAACGTCGATTGATGTTGTGCGAACCATGA
TCCCTAAACCGCAAACTGCAGCTCATACGGATTATTCCTTGGATGCGCAAGAGCTGGATCTGCTGCATCAAATTTTGAAT
GAAGCTGATGATGTCATCTACTCTGGCAATAACGCGATTGATTTGCTGCTCACCTCGATTGATGAAAACCGTGTCTCTCC
GGCGAGTTTTAAAAAACACTCAGTGGTAGATGTGATTGAAAAAGCAGTGAAAACTTTTCCGTATAAAAATGCAGCGGACC
AGCACTCGGTAGAGCTTGAAGTGCATCAGCCGTTTGACTTTTTTGGCAGTGATACTCTACTGACTTATGCGCTATTTAAC
CTGTTGAAAAATGCCTTCTATTACCAAAAAGAGCATTTCTCGGTTTGTATTTCCATTGAACAAACCAGTGAGCATAACCT
TATTCGAGTCCGTGATAACGGTGTGGGGATTGCGCCAGAGATGTTGGAAGACATTTTCCGTGATTTCTATACCTTCGGTA
AAAATGGTAGCTATGGGCTAGGATTGCCATTTTGCCGCAAAGTGATGTCCGCATTTGGGGGGACGATTCGCTGTGCGTCT
CAACAAGGTCAATGGACAGAATTCGTATTGAGCTTTCCTCGTTACGATTCCGATACGGTTAACGAGATAAAAACAGAACT
GCTGAAAACCAAATCGCTCATTTATATCGGCTCAAATCAAGCTATCGTTCGAGAGTTAAATCAGCTCGCAGTAGAAGATG
AGTTTGGTTTTACCGCTATTTCTGCGCAGCAAGCAGTAAGACGCCAAGATTATGAGTTTGAGTTCGACCTTATTTTGCTC
GACCTTGATGACGCTACGGCGCAGGGAGAACTACTGCCGAAACTTGAAGGGACATTGAGTTTTGCAGAAGGCTGTATCGG
CTATGTGTATGATCCGGGCAAAACGTATGCAGTAAACATTAACCGTTATCTGCGTATCCAACCGATCAGTATTCACTCTA
TTCTCAGAAAGCCGCGGAAAATCATCGAACGCTTGTTGTTTGAGCAAGAATCCTTATCGATGAATCGTAACGTGATCCCC
TTGCAAAAGAGTCGGCATGAACGACGTATTTTGGTGGTTGACGATAATCAATCGATCCGAACTTTTACGGCTATTTTGCT
TGAGCAACAAGGTTATGAAGTGGTGCAAGCCAACGATGGTAGTGAAGTGCTCAAGCATATGGAGAGTCAAAACATCGATT
TAGTATTGATGGACATTGAAATGCCGAATGTCGGGGGATTAGAAGCGACGCGTTTAATTCGTAACTCTGAGCATGAGTAC
AAAAATATTCCGATCATTGGTTACACCGGTGATAACAGCCCCAAAACCTTGGCTCTGGTACAAACCTCTGGCATGAATGA
TTTCATTGTGAAGCCTGCTGACCGTGATGTATTGCTAAATAAAGTGGCAGCTTGGGTGTAG

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(570-682)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(312-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KM66

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Iftekhar Bin Naser et al. (2019) Vibrio cholerae strains with inactivated cqsS gene overproduce autoinducer-2 which enhances resuscitation of dormant environmental V. cholerae. PloS One 14(10):e0223226. [PMID: 31574121]
[2] Yunzhou Wei et al. (2012) Ligand and antagonist driven regulation of the Vibrio cholerae quorum-sensing receptor CqsS. Molecular Microbiology 83(6):1095-108. [PMID: 22295878]