Detailed information NTG0001164

Experimentally validated

Overview

• Name: luxQ
• Type: Regulator
• Locus tag: A1552VC_RS18025
• Genome accession: NZ_CP028895
• Coordinates: 680831..683404 (-)
• Length: 857 a.a.
• NCBI ID: WP_001026306.1
• Uniprot ID: Q9KLK7
• Organism: Vibrio cholerae strain A1552
• Function: autoinducer sensor Competence regulation

Function

Extracellular CAI‑1 is sensed by the sensor kinase CqsS, whereas AI‑2 is sensed by the LuxPQ receptor complex. at high cell densities both signals promote dephosphorylation of LuxO, thereby promoting HapR production.

Genomic Context

Location: 675831..688404

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A1552VC_RS18000 (A1552VC_A03432)	-	678254..678559 (-)	306	WP_001181134.1	hypothetical protein	-
A1552VC_RS18005 (A1552VC_A03433)	-	678762..679160 (+)	399	WP_000831416.1	YgiW/YdeI family stress tolerance OB fold protein	-
A1552VC_RS18015 (A1552VC_A03434)	-	679412..679930 (+)	519	WP_000914961.1	hypothetical protein	-
A1552VC_RS18020 (A1552VC_A03435)	-	680174..680839 (+)	666	WP_000268204.1	PilZ domain-containing protein	-
A1552VC_RS18025 (A1552VC_A03436)	luxQ	680831..683404 (-)	2574	WP_001026306.1	quorum-sensing autoinducer 2 sensor kinase/phosphatase LuxQ	Regulator
A1552VC_RS18030 (A1552VC_A03437)	luxP	683422..684516 (-)	1095	WP_000823678.1	autoinducer 2-binding periplasmic protein LuxP	Regulator
A1552VC_RS18035 (A1552VC_A03438)	traF	684728..685888 (-)	1161	WP_001025860.1	conjugal transfer protein TraF	-
A1552VC_RS18040 (A1552VC_A03439)	-	685990..686295 (-)	306	WP_000429067.1	GIY-YIG nuclease family protein	-
A1552VC_RS18045 (A1552VC_A03440)	-	686312..686962 (-)	651	WP_001025631.1	YceH family protein	-
A1552VC_RS18050 (A1552VC_A03441)	-	687004..687201 (-)	198	WP_001246071.1	hypothetical protein	-
A1552VC_RS18055 (A1552VC_A03442)	-	687389..687709 (+)	321	WP_001089529.1	DUF496 family protein	-
A1552VC_RS18065 (A1552VC_A03444)	-	688122..688373 (+)	252	WP_001882582.1	YgjV family protein	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
luxQ	luxO	positive effect
luxO	hapR	positive effect
cqsS	luxO	positive effect
cqsA	cqsS	positive effect
luxS	luxQ	positive effect
luxP	luxO	positive effect
luxS	luxP	positive effect

Sequence

Protein

• Length: 857 a.a.
• Molecular weight: 96929.73 Da
• Isoelectric Point: 6.0197

>NTDB_id=1164 A1552VC_RS18025 WP_001026306.1 680831..683404(-) (luxQ) [Vibrio cholerae strain A1552]
MNIRPSQIKHKQRIASFITHAVVVVMGVLIVSVLFQSYQISSRLMAQEGQRTSVQTSSLIQSLFDFRLAALRIHQDSTAK
NASLINALVSRDSSRLDEFFSSVDELELSNAPDLRFISSHDNILWDDGNASFYGIAQQELNKLIRRVAISGNWHLVQTPS
EGKSVHILMRRSSLIEAGTGQVVGYLYVGIVLNDNFALLENIRSGSNSENLVLAVDTTPLVSTLKGNEPYSLDYVVHSAK
DAMRDSFIVGQTFLEVESVPTYLCVYSIQTNQNVLTLRDNFYFWMAFALISMIGVSIASRWWLQKRIQREIETLMNYTHK
LMDLDTKSEFIGSKIYEFDYFGRTLEQSFRRLANKEKQFEDLFNFALSPTMLWNTSGRLIRMNPSAQIQFLREDAQNHFL
FEILERQLLPTITNAAQGNNPSDVTTEVDGRVYRWNLSPIMVEGQIISIITQGQDITTIAEAEKQSQAARREAEESARVR
AEFLAKMSHELRTPLNGVLGVSQLLKRTPLNDEQREHVAVLCSSGEHLLAVLNDILDFSRLEQGKFRIQKNEFRLKELVC
AIDRIYRPLCNEKGLELVVNSNITTAAIVRSDQIRINQILFNLLNNAIKFTHQGSIRVELQLIEGDPLAQLVIQVVDTGI
GIREQDLTVIFEPFMQAESTTTREYGGSGLGLTIVHSLVEMLSGQLHVSSEYGIGTRFEIQLPIELVEKPDAPQQLLPAP
DPQPLFDKTLRVLLVEDNHTNAFIAQAFCRKYGLDVSWVTDGLQAIEELKIHDYDLVLMDNQLPYLDGVETTRTIKKVLH
LPVVVYACTADGLEETRQAFFHAGAEYVLVKPLKEQTLHKALEHFKHHHGQKNAGLN

Nucleotide

• Length: 2574 bp

>NTDB_id=1164 A1552VC_RS18025 WP_001026306.1 680831..683404(-) (luxQ) [Vibrio cholerae strain A1552]
TTGAACATTCGACCTAGCCAAATCAAGCATAAACAGCGCATCGCTTCTTTTATCACCCATGCTGTCGTGGTGGTGATGGG
CGTTCTGATCGTCAGTGTATTATTCCAAAGTTACCAGATCAGTAGCCGCTTGATGGCTCAAGAAGGACAACGCACTTCAG
TTCAAACCTCCAGCTTGATTCAAAGTTTGTTTGATTTTCGTCTTGCGGCACTGCGAATTCACCAGGACAGCACAGCCAAG
AACGCCAGTTTAATCAACGCACTTGTCAGCCGAGATTCGAGTCGGCTGGATGAATTCTTTTCCAGTGTTGATGAACTTGA
ACTCTCTAATGCGCCAGATCTGCGTTTTATCTCCAGTCACGACAACATCTTGTGGGATGATGGCAACGCCTCTTTTTACG
GCATTGCCCAGCAAGAGCTGAATAAGCTTATCCGCAGAGTGGCGATTAGTGGCAATTGGCACTTGGTTCAAACGCCCAGC
GAGGGAAAATCAGTCCATATTTTGATGCGTCGTTCATCGCTGATTGAAGCGGGAACCGGGCAGGTTGTTGGGTATCTGTA
TGTAGGCATCGTACTCAATGATAACTTTGCGCTGTTGGAAAATATCCGCAGTGGCAGTAACTCAGAAAACCTAGTCCTTG
CCGTGGATACTACGCCGCTGGTTTCTACCTTGAAAGGCAATGAACCTTATTCGCTGGACTATGTGGTGCACAGCGCCAAA
GATGCTATGCGAGACAGCTTTATTGTTGGGCAAACCTTCTTAGAAGTGGAAAGTGTACCGACCTATCTGTGCGTCTACTC
GATTCAGACCAACCAAAATGTGCTCACCCTGCGGGATAATTTCTATTTTTGGATGGCGTTTGCGCTGATCAGTATGATCG
GTGTTTCCATAGCGTCACGCTGGTGGCTACAAAAACGCATTCAGCGTGAAATTGAAACCTTGATGAATTACACCCATAAA
TTGATGGATCTGGATACGAAAAGTGAGTTTATCGGTTCAAAAATTTATGAGTTCGACTATTTTGGGCGAACGTTGGAACA
ATCATTCCGTCGTCTCGCCAATAAAGAAAAGCAGTTTGAAGATCTGTTTAACTTTGCGCTTTCACCGACCATGCTTTGGA
ATACCTCTGGTCGGCTGATCCGCATGAACCCCTCAGCGCAAATCCAGTTTTTGCGCGAAGATGCGCAAAACCATTTCTTA
TTTGAGATTCTTGAACGCCAACTGTTGCCGACCATCACTAATGCGGCACAGGGCAATAATCCGAGTGACGTCACAACCGA
AGTGGATGGTCGTGTGTACCGCTGGAACCTTTCACCGATCATGGTGGAAGGGCAGATCATTTCCATCATTACTCAAGGGC
AAGATATCACCACCATCGCGGAAGCGGAGAAGCAAAGCCAAGCCGCAAGGCGCGAAGCGGAAGAGTCGGCACGGGTCAGG
GCCGAGTTTTTGGCCAAAATGAGTCACGAATTGCGCACGCCGCTCAATGGTGTGCTCGGTGTTTCCCAACTGCTAAAACG
GACCCCACTCAATGATGAACAGCGCGAACACGTGGCTGTGTTATGCAGCAGTGGTGAACATTTGCTCGCGGTGCTGAATG
ACATCTTGGATTTCTCACGCTTAGAGCAGGGCAAATTCCGTATTCAAAAGAATGAGTTTCGCCTTAAAGAGTTGGTGTGC
GCGATTGACCGCATTTATCGACCTTTATGTAATGAAAAAGGCTTGGAACTGGTGGTGAATTCCAATATCACTACCGCAGC
GATTGTGCGCAGCGATCAGATCCGGATTAACCAAATCTTGTTCAATTTGCTCAATAACGCGATCAAGTTTACTCACCAAG
GTTCGATACGTGTCGAATTGCAATTAATTGAAGGTGATCCATTAGCTCAGCTGGTGATTCAAGTCGTCGATACGGGGATT
GGTATCCGCGAACAAGATTTAACGGTGATTTTTGAACCCTTCATGCAAGCTGAATCGACCACCACTCGAGAGTACGGCGG
AAGTGGTTTAGGCTTAACGATTGTGCACAGTTTAGTGGAAATGCTCAGTGGTCAACTGCACGTCAGCTCCGAGTATGGCA
TTGGTACACGTTTTGAAATTCAGTTACCGATTGAGCTGGTAGAAAAACCGGATGCGCCGCAGCAGTTGTTACCAGCTCCT
GATCCTCAACCGTTATTTGATAAAACTCTGCGTGTACTGTTGGTGGAGGATAACCACACCAATGCCTTTATTGCTCAAGC
ATTCTGTCGTAAATATGGCTTAGATGTGAGCTGGGTAACGGATGGACTACAGGCGATTGAAGAGCTCAAAATACATGACT
ACGACTTAGTGCTGATGGATAACCAGTTACCCTATTTGGATGGCGTTGAGACCACGCGCACCATCAAAAAAGTGCTGCAC
CTTCCGGTGGTGGTGTATGCCTGCACTGCGGATGGCTTAGAAGAAACACGGCAAGCATTTTTCCATGCGGGCGCGGAATA
TGTGTTAGTGAAGCCACTGAAAGAGCAGACTTTGCATAAAGCGCTGGAGCATTTCAAACATCATCATGGCCAAAAAAACG
CTGGCTTAAATTGA

Domains

Predicted by InterproScan.

• LuxQ, periplasmic (LuxQ-periplasm) (41-277)

• Response regulator receiver domain (Response_reg) (732-842)

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (592-705)

• His Kinase A (phospho-acceptor) domain (HisKA) (480-544)

3D structure

• PDB: 3C30
• PDB: 3C38

References

• [1]: Calum Johnston et al. (2014) Bacterial transformation: distribution, shared mechanisms and divergent control. Nature Reviews. Microbiology 12(3):181-96. [PMID: 24509783]