Detailed information    

experimental Experimentally validated

Overview

Name   cqsA   Type   Regulator
Locus tag   A1552VC_RS17030 Genome accession   NZ_CP028895
Coordinates   457437..458606 (-) Length   389 a.a.
NCBI ID   WP_001039912.1    Uniprot ID   Q9KM65
Organism   Vibrio cholerae strain A1552     
Function   induction of competence   
Competence regulation

Function

The cqsA gene encodes the cholerae autoinducer 1 (CAI-1), which is involved in the induction of competence in Vibrio cholerae.


Genomic Context

Location: 452437..463606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS17020 (A1552VC_A03239) cqsS 455148..457208 (+) 2061 WP_001882375.1 quorum-sensing CAI-1 autoinducer sensor kinase/phosphatase CqsS Regulator
  A1552VC_RS17030 (A1552VC_A03240) cqsA 457437..458606 (-) 1170 WP_001039912.1 alpha-hydroxyketone-type quorum-sensing autoinducer synthase Regulator
  A1552VC_RS17035 (A1552VC_A03241) - 458803..459765 (-) 963 WP_001104009.1 TDT family transporter -
  A1552VC_RS17040 (A1552VC_A03242) clcA 459912..461318 (-) 1407 WP_000107451.1 H(+)/Cl(-) exchange transporter ClcA -
  A1552VC_RS17045 (A1552VC_A03243) - 461785..462774 (+) 990 WP_000610709.1 CobW family GTP-binding protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  cqsA cqsS positive effect
  cqsS luxO positive effect
  luxO hapR positive effect
  luxQ luxO positive effect
  luxP luxO positive effect
  luxS luxQ positive effect
  luxS luxP positive effect

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 43593.61 Da        Isoelectric Point: 6.2272

>NTDB_id=1155 A1552VC_RS17030 WP_001039912.1 457437..458606(-) (cqsA) [Vibrio cholerae strain A1552]
MNKPQLPDFIQNKIDHYIENYFDINKNGKHLVLGKQASPDDIILQSNDYLALANHPLIKARLAKSLLEEQQSLFMSASFL
QNDYDKPMIEKRLAKFTGFDECLLSQSGWNANVGLLQTICQPNTNVYIDFFAHMSLWEGARYANAQAHPFMHNNCDHLRM
LIQRHGPGIIVVDSIYSTLGTIAPLAELVNISKEFGCALLVDESHSLGTHGPNGAGLLAELGLTREVHFMTASLAKTFAY
RAGAIWCNNEVNRCVPFISYPAIFSSTLLPYEAAGLETTLEIIESADNRRQHLDRMARKLRIGLSQLGLTIRSESQIIGL
ETGDERNTEKVRDYLESNGVFGSVFCRPATSKNKNIIRLSLNSDVNDEQIAKIIEVCSDAVNYGDFYFR

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=1155 A1552VC_RS17030 WP_001039912.1 457437..458606(-) (cqsA) [Vibrio cholerae strain A1552]
ATGAACAAGCCTCAACTTCCTGATTTTATTCAGAACAAGATAGATCACTATATTGAAAATTATTTTGATATAAACAAAAA
CGGTAAACACCTTGTATTGGGTAAACAAGCCAGCCCTGATGACATTATTTTGCAAAGTAATGATTATCTCGCATTGGCCA
ATCATCCGTTGATCAAAGCTCGTTTGGCGAAGTCATTACTGGAAGAACAACAAAGCTTATTTATGTCAGCCTCATTTCTA
CAAAATGACTATGACAAACCCATGATTGAGAAACGTCTGGCTAAGTTCACAGGCTTTGATGAATGTCTATTATCTCAATC
TGGTTGGAATGCAAACGTCGGTTTATTACAAACCATCTGCCAGCCCAATACGAATGTTTACATTGATTTTTTCGCGCACA
TGTCGTTATGGGAAGGGGCGCGCTACGCCAATGCTCAGGCGCATCCTTTTATGCATAATAACTGTGACCATTTACGTATG
CTGATTCAACGTCATGGTCCTGGGATCATTGTCGTAGACTCGATTTACAGCACTTTAGGTACGATTGCACCGCTAGCGGA
ACTGGTCAATATCAGTAAAGAGTTTGGCTGCGCCTTATTAGTCGATGAATCCCACTCTTTGGGCACACATGGCCCTAATG
GTGCAGGTTTATTGGCAGAATTAGGCCTCACTCGTGAAGTGCATTTTATGACCGCAAGTTTGGCCAAAACCTTTGCTTAT
CGCGCAGGAGCCATTTGGTGTAACAATGAAGTGAATCGCTGCGTTCCTTTTATTAGTTATCCAGCTATTTTTAGTTCTAC
TTTGCTGCCTTATGAAGCGGCAGGATTAGAAACGACTTTAGAGATTATTGAATCTGCGGATAATCGTCGTCAGCATTTAG
ATCGTATGGCAAGAAAATTACGCATAGGATTATCCCAGCTGGGATTAACCATTCGCAGTGAAAGCCAAATTATTGGTCTA
GAAACAGGAGATGAACGAAATACCGAAAAAGTTCGGGATTATTTAGAAAGTAATGGAGTGTTTGGCTCAGTATTCTGCCG
CCCGGCAACTTCAAAGAATAAAAACATTATTCGCTTATCACTCAATAGTGATGTGAACGATGAGCAAATCGCCAAAATAA
TTGAGGTTTGCTCTGATGCGGTCAACTACGGTGATTTTTATTTTCGTTAA

Domains

Predicted by InterproScan.

Aminotransferase class I and II (Aminotran_1_2)

(41-375)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 2WK7
  PDB 2WK8
  PDB 2WK9
  PDB 2WKA
  PDB 3HQT
  PDB 3KKI

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Sandrine Stutzmann et al. (2020) Comparison of chitin-induced natural transformation in pandemic Vibrio cholerae O1 El Tor strains. Environmental Microbiology 22(10):4149-4166. [PMID: 32860313]
[2] Mirella Lo Scrudato et al. (2012) The regulatory network of natural competence and transformation of Vibrio cholerae. PLoS Genetics 8(6):e1002778. [PMID: 22737089]
[3] Yunzhou Wei et al. (2011) Mechanism of Vibrio cholerae autoinducer-1 biosynthesis. ACS Chemical Biology 6(4):356-65. [PMID: 21197957]
[4] Gaia Suckow et al. (2011) Quorum sensing contributes to natural transformation of Vibrio cholerae in a species-specific manner. Journal of Bacteriology 193(18):4914-24. [PMID: 21784943]
[5] Nasrin Jahan et al. (2009) Insights into the biosynthesis of the Vibrio cholerae major autoinducer CAI-1 from the crystal structure of the PLP-dependent enzyme CqsA. Journal of Molecular Biology 392(3):763-73. [PMID: 19631226]
[6] Robert C Kelly et al. (2009) The Vibrio cholerae quorum-sensing autoinducer CAI-1: analysis of the biosynthetic enzyme CqsA. Nature Chemical Biology 5(12):891-5. [PMID: 19838203]