Detailed information    

experimental Experimentally validated

Overview

Name   luxP   Type   Regulator
Locus tag   A1552VC_RS18030 Genome accession   NZ_CP028895
Coordinates   683422..684516 (-) Length   364 a.a.
NCBI ID   WP_000823678.1    Uniprot ID   Q9KLK6
Organism   Vibrio cholerae strain A1552     
Function   autoinducer sensor   
Competence regulation

Function

Extracellular CAI‑1 is sensed by the sensor kinase CqsS, whereas AI‑2 is sensed by the LuxPQ receptor complex. at high cell densities both signals promote dephosphorylation of LuxO, thereby promoting HapR production.


Genomic Context

Location: 678422..689516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS18005 (A1552VC_A03433) - 678762..679160 (+) 399 WP_000831416.1 YgiW/YdeI family stress tolerance OB fold protein -
  A1552VC_RS18015 (A1552VC_A03434) - 679412..679930 (+) 519 WP_000914961.1 hypothetical protein -
  A1552VC_RS18020 (A1552VC_A03435) - 680174..680839 (+) 666 WP_000268204.1 PilZ domain-containing protein -
  A1552VC_RS18025 (A1552VC_A03436) luxQ 680831..683404 (-) 2574 WP_001026306.1 quorum-sensing autoinducer 2 sensor kinase/phosphatase LuxQ Regulator
  A1552VC_RS18030 (A1552VC_A03437) luxP 683422..684516 (-) 1095 WP_000823678.1 autoinducer 2-binding periplasmic protein LuxP Regulator
  A1552VC_RS18035 (A1552VC_A03438) traF 684728..685888 (-) 1161 WP_001025860.1 conjugal transfer protein TraF -
  A1552VC_RS18040 (A1552VC_A03439) - 685990..686295 (-) 306 WP_000429067.1 GIY-YIG nuclease family protein -
  A1552VC_RS18045 (A1552VC_A03440) - 686312..686962 (-) 651 WP_001025631.1 YceH family protein -
  A1552VC_RS18050 (A1552VC_A03441) - 687004..687201 (-) 198 WP_001246071.1 hypothetical protein -
  A1552VC_RS18055 (A1552VC_A03442) - 687389..687709 (+) 321 WP_001089529.1 DUF496 family protein -
  A1552VC_RS18065 (A1552VC_A03444) - 688122..688373 (+) 252 WP_001882582.1 YgjV family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  luxP luxO positive effect
  luxO hapR positive effect
  cqsS luxO positive effect
  cqsA cqsS positive effect
  luxQ luxO positive effect
  luxS luxQ positive effect
  luxS luxP positive effect

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 41196.81 Da        Isoelectric Point: 6.3129

>NTDB_id=1163 A1552VC_RS18030 WP_000823678.1 683422..684516(-) (luxP) [Vibrio cholerae strain A1552]
MKRKLISPLFLIAALFSSTGQADSLKGYWHYDEFLAEFPEQRPISELFAQTVRDKPSALTVAQEQPVIISVVYPGQQISD
YWVRNIKAFEKRMDALGIRYQINQVFTRPNLDTRQQSVSLMEALKNKSDYLIFTLDTTRHRKFIEHVLHSSETKLILQNI
TTPVKDWQGKQPMMYIGFDHEYGAQLLAQYYQAHAPKKPYSVLYFSEGYISEARGDTFIQDMNAVHHFPLASSYYTKATE
QSGYEATLNIVKNNPEIGFIYACATDVALGAAQALKELNRQDILVNGWGGGSAELEALAKGELDVTVMRMNDDTGVAMAE
AIKWDLEGKSVPLVYSGDFEVVTKQDSAARIAELKARAFRYSDR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=1163 A1552VC_RS18030 WP_000823678.1 683422..684516(-) (luxP) [Vibrio cholerae strain A1552]
ATGAAAAGAAAGCTAATATCTCCGCTGTTTCTGATAGCAGCACTCTTCAGCTCAACTGGTCAAGCCGATTCATTAAAAGG
CTATTGGCATTATGATGAATTTCTTGCTGAGTTCCCTGAACAGCGGCCAATTTCCGAGCTTTTTGCTCAAACAGTTCGTG
ATAAACCGAGTGCGTTGACGGTGGCGCAAGAACAACCCGTCATTATTTCGGTGGTCTATCCCGGACAGCAAATTTCTGAC
TATTGGGTTCGCAACATCAAAGCGTTTGAAAAGCGAATGGATGCTTTGGGGATTCGCTATCAAATCAATCAGGTGTTTAC
TCGCCCGAATCTTGATACTCGCCAGCAGAGTGTTTCCTTGATGGAAGCGCTGAAAAATAAGAGCGACTATCTGATTTTTA
CCTTAGATACCACCCGTCACCGCAAATTCATTGAGCATGTGCTGCACTCCTCCGAAACGAAACTGATTTTGCAAAACATC
ACGACGCCAGTCAAAGATTGGCAAGGCAAGCAGCCCATGATGTACATCGGTTTTGATCATGAGTACGGTGCGCAATTATT
GGCGCAATATTACCAAGCACATGCTCCGAAAAAGCCGTACTCAGTACTTTATTTTTCCGAGGGGTATATCAGTGAAGCGC
GGGGAGACACCTTCATTCAAGATATGAACGCGGTGCACCATTTTCCGTTGGCTTCTTCCTATTACACCAAGGCAACCGAA
CAAAGTGGCTATGAAGCGACCCTAAACATCGTCAAGAATAATCCAGAAATTGGCTTTATTTACGCCTGTGCGACGGACGT
CGCTTTGGGAGCTGCGCAGGCACTGAAAGAGCTGAATCGACAAGATATTCTGGTCAATGGTTGGGGAGGCGGTTCGGCAG
AGCTTGAAGCGCTTGCCAAAGGAGAATTGGATGTCACTGTGATGCGTATGAATGACGATACGGGTGTGGCGATGGCTGAA
GCGATTAAATGGGATTTGGAAGGGAAATCCGTACCTTTGGTCTATTCAGGCGATTTTGAAGTGGTCACCAAACAAGATTC
AGCAGCACGTATTGCAGAACTGAAAGCTCGGGCTTTCCGTTATTCGGATCGTTAG

Domains

Predicted by InterproScan.

Periplasmic binding protein domain (Peripla_BP_4)

(69-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KLK6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Calum Johnston et al. (2014) Bacterial transformation: distribution, shared mechanisms and divergent control. Nature Reviews. Microbiology 12(3):181-96. [PMID: 24509783]