Detailed information    

experimental Experimentally validated

Overview

Name   dns   Type   Auxiliary factor
Locus tag   VF_RS02230 Genome accession   NC_006840
Coordinates   464082..464786 (-) Length   234 a.a.
NCBI ID   WP_011261220.1    Uniprot ID   Q5E7R4
Organism   Aliivibrio fischeri ES114     
Function   degradation of extracellular DNA   
Defense system

Function

Inactivation of dns increased transformation efficiency by ~2 orders of magnitude.


Genomic Context

Location: 459082..469786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VF_RS02205 (VF0432) pilU 459978..461078 (+) 1101 WP_011261216.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VF_RS02210 (VF0433) ruvX 461218..461643 (-) 426 WP_011261217.1 Holliday junction resolvase RuvX -
  VF_RS02215 (VF0434) - 461640..462203 (-) 564 WP_005417575.1 YqgE/AlgH family protein -
  VF_RS02220 (VF0435) gshB 462316..463263 (-) 948 WP_011261218.1 glutathione synthase -
  VF_RS02225 (VF0436) rsmE 463277..464008 (-) 732 WP_011261219.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VF_RS02230 (VF0437) dns 464082..464786 (-) 705 WP_011261220.1 endonuclease Auxiliary factor
  VF_RS02235 (VF0438) - 464878..465378 (-) 501 WP_011261221.1 SprT family zinc-dependent metalloprotease -
  VF_RS02240 (VF0439) metK 465544..466698 (-) 1155 WP_005417583.1 methionine adenosyltransferase -
  VF_RS02245 (VF0440) tkt 467063..469057 (+) 1995 WP_011261222.1 transketolase -

Sequence

Protein


Download         Length: 234 a.a.        Molecular weight: 27182.13 Da        Isoelectric Point: 10.1788

>NTDB_id=1303 VF_RS02230 WP_011261220.1 464082..464786(-) (dns) [Aliivibrio fischeri ES114]
MKLIRLLISIIAIGSAFNVLAAPPSSFSKAKKEAVKIYLDHPTSFYCGCDITWKDKKKGIPDLQSCGYNVRKQEKRASRI
EWEHVVPAWQFGHQRQCWQDGGRKNCTRKDKQFKLMEADLHNLVPAIGEVNGDRSNFRFSQWNGNKGAYYGQCAFKVDFK
GRVAEPPAQSRGAIARTYLYMNQEYRFNLSKSQRQLMNAWDKQYPVSEWECERDKRIAKIQGNHNQFVYKACRK

Nucleotide


Download         Length: 705 bp        

>NTDB_id=1303 VF_RS02230 WP_011261220.1 464082..464786(-) (dns) [Aliivibrio fischeri ES114]
ATGAAATTAATCCGCTTACTTATTAGCATTATCGCTATTGGCTCTGCATTTAATGTATTAGCCGCTCCTCCTTCTTCATT
TTCAAAAGCCAAAAAAGAAGCTGTCAAAATCTATTTAGATCATCCAACCTCATTTTATTGTGGCTGTGACATAACTTGGA
AAGATAAAAAGAAAGGCATTCCTGATTTACAAAGTTGTGGTTATAACGTGCGTAAACAAGAAAAGCGAGCAAGCCGAATC
GAATGGGAGCACGTCGTTCCAGCATGGCAATTTGGCCACCAACGTCAATGTTGGCAAGATGGGGGCCGTAAAAACTGCAC
CCGAAAAGATAAGCAATTTAAGTTAATGGAAGCTGATTTACATAACCTTGTTCCAGCAATTGGCGAAGTAAATGGTGACC
GATCTAATTTCCGTTTCTCACAATGGAATGGCAATAAAGGCGCATATTACGGTCAGTGTGCCTTCAAAGTGGATTTCAAA
GGACGTGTCGCTGAGCCACCAGCACAATCAAGAGGTGCTATTGCTCGTACATATTTATACATGAATCAAGAGTATCGTTT
CAATTTATCGAAGTCACAACGTCAGCTAATGAATGCATGGGACAAGCAATACCCAGTATCTGAATGGGAGTGTGAACGCG
ATAAGCGCATTGCTAAAATTCAAGGCAACCATAACCAGTTTGTCTACAAAGCATGTCGCAAATAG

Domains

Predicted by InterproScan.

Endonuclease I (Endonuclease_1)

(34-231)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5E7R4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dns Vibrio parahaemolyticus RIMD 2210633

70.815

100

0.714
  dns Vibrio cholerae strain A1552

67.241

100

0.675
  ideA Vibrio cholerae O1 str. 2010EL-1786

52.609

100

0.533
  dns Campylobacter jejuni RM1221

43.421

100

0.444

Multiple sequence alignment    



References

[1] Pat M Fidopiastis et al. (2022) Vibrio fischeri Possesses Xds and Dns Nucleases That Differentially Influence Phosphate Scavenging, Aggregation, Competence, and Symbiotic Colonization of Squid. Applied And Environmental Microbiology 88(22):e0163522. [PMID: 36342139]