Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VF_RS02205 Genome accession   NC_006840
Coordinates   459978..461078 (+) Length   366 a.a.
NCBI ID   WP_011261216.1    Uniprot ID   Q5E7R9
Organism   Aliivibrio fischeri ES114     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 454978..466078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VF_RS02170 (VF0425) - 455296..455892 (-) 597 WP_011261211.1 XTP/dITP diphosphatase -
  VF_RS02175 (VF0426) - 455903..456334 (-) 432 WP_011261212.1 DUF4426 domain-containing protein -
  VF_RS02180 (VF0427) yggU 456404..456691 (-) 288 WP_005417561.1 DUF167 family protein YggU -
  VF_RS02185 (VF0428) - 456699..457253 (-) 555 WP_011261213.1 YggT family protein -
  VF_RS02190 (VF0429) proC 457302..458120 (-) 819 WP_011261214.1 pyrroline-5-carboxylate reductase -
  VF_RS02195 (VF0430) - 458204..458914 (-) 711 WP_011261215.1 YggS family pyridoxal phosphate-dependent enzyme -
  VF_RS02200 (VF0431) pilT 458938..459975 (+) 1038 WP_005417568.1 type IV pilus twitching motility protein PilT Machinery gene
  VF_RS02205 (VF0432) pilU 459978..461078 (+) 1101 WP_011261216.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VF_RS02210 (VF0433) ruvX 461218..461643 (-) 426 WP_011261217.1 Holliday junction resolvase RuvX -
  VF_RS02215 (VF0434) - 461640..462203 (-) 564 WP_005417575.1 YqgE/AlgH family protein -
  VF_RS02220 (VF0435) gshB 462316..463263 (-) 948 WP_011261218.1 glutathione synthase -
  VF_RS02225 (VF0436) rsmE 463277..464008 (-) 732 WP_011261219.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VF_RS02230 (VF0437) dns 464082..464786 (-) 705 WP_011261220.1 endonuclease Auxiliary factor
  VF_RS02235 (VF0438) - 464878..465378 (-) 501 WP_011261221.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 41416.75 Da        Isoelectric Point: 6.3737

>NTDB_id=24243 VF_RS02205 WP_011261216.1 459978..461078(+) (pilU) [Aliivibrio fischeri ES114]
MTLDQILKQMITLKASDIYLTVDAPCLLRVNGELQALGHKQSIESVTALLAEMMDEERQEEFNESLEANFAIVKEFGRFR
VSAFWQREQPGAVIRRIETKIPSMESLNLPEVMKELAVTKRGLVLVVGATGSGKSTSMAAMTGYRNDNRSGHILTVEDPI
EFVHPHNKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRNRETMEYAMSFAETGHLCMATLHANNANQALERILH
LVPKESKEQFLFDLSMNLKGVIAQQLVPDMNGNGRHGVYEVLLNTPRVADLIRRGDLHELKSTMAKSRESGMQTFDQSLF
ELVMDKKISEMEALHSADSANDLRLMLKTKEGMRSLHLHWIMCRYR

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=24243 VF_RS02205 WP_011261216.1 459978..461078(+) (pilU) [Aliivibrio fischeri ES114]
ATGACATTGGATCAAATACTGAAACAAATGATCACTTTAAAAGCATCGGATATTTACTTAACGGTTGATGCTCCTTGCTT
GCTGAGAGTGAATGGTGAACTTCAAGCTTTAGGACATAAACAGAGTATTGAATCAGTCACCGCTTTGCTTGCTGAGATGA
TGGATGAAGAGCGCCAAGAAGAGTTTAATGAAAGCTTAGAAGCGAACTTTGCCATTGTGAAAGAATTTGGTCGCTTTCGT
GTGAGTGCTTTTTGGCAGCGAGAGCAACCTGGTGCTGTGATACGTCGTATTGAAACCAAAATCCCATCCATGGAGTCACT
AAATCTTCCAGAAGTAATGAAAGAATTAGCCGTTACTAAGCGTGGATTAGTATTAGTGGTAGGGGCAACGGGCTCTGGTA
AATCAACATCAATGGCTGCGATGACGGGGTATCGAAATGACAACCGAAGTGGTCATATCTTAACGGTAGAAGACCCAATT
GAGTTTGTGCATCCTCATAATAAATGCATCGTGACTCAGCGTGAAGTTGGCCTTGATACTGAAAGTTATGAGGTGGCATT
AAAAAACTCGTTACGCCAAGCTCCAGATATGATTTTAATTGGTGAGATCCGTAATCGAGAGACGATGGAATATGCCATGA
GCTTTGCAGAGACAGGGCATCTATGCATGGCAACATTACACGCAAATAACGCAAACCAAGCATTAGAGCGAATTTTGCAC
CTTGTTCCTAAAGAGAGTAAAGAGCAGTTTTTGTTTGATTTATCCATGAACTTAAAAGGGGTCATTGCACAGCAGCTAGT
TCCGGATATGAATGGTAATGGTCGTCATGGCGTTTATGAAGTGTTACTAAACACGCCACGCGTTGCAGACTTGATCCGTC
GTGGAGATTTGCATGAACTTAAATCGACCATGGCAAAATCACGTGAGTCGGGTATGCAGACGTTTGATCAGAGTTTATTT
GAACTGGTAATGGATAAGAAAATATCAGAAATGGAAGCGCTGCACAGTGCGGATTCGGCTAATGATTTACGCCTGATGTT
GAAAACAAAAGAGGGGATGCGTTCTCTTCATCTACATTGGATAATGTGTCGATATCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5E7R9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

77.557

96.175

0.746
  pilU Pseudomonas stutzeri DSM 10701

57.429

95.628

0.549
  pilU Acinetobacter baylyi ADP1

53.107

96.721

0.514
  pilT Legionella pneumophila strain ERS1305867

41.691

93.716

0.391
  pilT Legionella pneumophila strain Lp02

41.691

93.716

0.391
  pilT Acinetobacter baumannii D1279779

41.108

93.716

0.385
  pilT Acinetobacter baumannii strain A118

41.108

93.716

0.385
  pilT Acinetobacter nosocomialis M2

41.108

93.716

0.385
  pilT Pseudomonas aeruginosa PAK

42.249

89.891

0.38
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

87.978

0.377
  pilT Pseudomonas stutzeri DSM 10701

41.641

89.891

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.538

88.798

0.369
  pilT Vibrio cholerae strain A1552

41.538

88.798

0.369

Multiple sequence alignment