Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VF_RS02200 Genome accession   NC_006840
Coordinates   458938..459975 (+) Length   345 a.a.
NCBI ID   WP_005417568.1    Uniprot ID   A0AAV3EWN5
Organism   Aliivibrio fischeri ES114     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 453938..464975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VF_RS02165 (VF0424) hemW 454132..455292 (-) 1161 WP_011261210.1 radical SAM family heme chaperone HemW -
  VF_RS02170 (VF0425) - 455296..455892 (-) 597 WP_011261211.1 XTP/dITP diphosphatase -
  VF_RS02175 (VF0426) - 455903..456334 (-) 432 WP_011261212.1 DUF4426 domain-containing protein -
  VF_RS02180 (VF0427) yggU 456404..456691 (-) 288 WP_005417561.1 DUF167 family protein YggU -
  VF_RS02185 (VF0428) - 456699..457253 (-) 555 WP_011261213.1 YggT family protein -
  VF_RS02190 (VF0429) proC 457302..458120 (-) 819 WP_011261214.1 pyrroline-5-carboxylate reductase -
  VF_RS02195 (VF0430) - 458204..458914 (-) 711 WP_011261215.1 YggS family pyridoxal phosphate-dependent enzyme -
  VF_RS02200 (VF0431) pilT 458938..459975 (+) 1038 WP_005417568.1 type IV pilus twitching motility protein PilT Machinery gene
  VF_RS02205 (VF0432) pilU 459978..461078 (+) 1101 WP_011261216.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VF_RS02210 (VF0433) ruvX 461218..461643 (-) 426 WP_011261217.1 Holliday junction resolvase RuvX -
  VF_RS02215 (VF0434) - 461640..462203 (-) 564 WP_005417575.1 YqgE/AlgH family protein -
  VF_RS02220 (VF0435) gshB 462316..463263 (-) 948 WP_011261218.1 glutathione synthase -
  VF_RS02225 (VF0436) rsmE 463277..464008 (-) 732 WP_011261219.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VF_RS02230 (VF0437) dns 464082..464786 (-) 705 WP_011261220.1 endonuclease Auxiliary factor

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38136.77 Da        Isoelectric Point: 6.2402

>NTDB_id=24242 VF_RS02200 WP_005417568.1 458938..459975(+) (pilT) [Aliivibrio fischeri ES114]
MDITELLDFSVKQNASDLHLSAGVPPMVRVDGEVRKLTLPDFTHADVHRLVTDIMNDAQRSEFEDKLEVDFSFELPNIGR
FRVNAFNQSRGCAAVFRTIPVDIPSLEQLAAPQVFTDIAKYQKGLVLITGPTGSGKSTTLAAMVDYINENYNKHVLTIED
PIEFVHKNKKCLINQREVNRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKSMVRSMLSESLRAVVAQTLLKRIGGGRIACHEIMIATPAIRNLIREDKVAQMYSIIQTGSSFGMNTMEQNA
KKIIAQGLVDPDEVVKKVEMSSIGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=24242 VF_RS02200 WP_005417568.1 458938..459975(+) (pilT) [Aliivibrio fischeri ES114]
ATGGACATCACAGAACTACTGGACTTTAGTGTAAAACAAAACGCATCAGATCTACACCTATCTGCAGGAGTTCCTCCTAT
GGTACGTGTAGATGGTGAAGTGCGTAAACTGACATTACCTGATTTTACACATGCCGATGTTCATCGTTTAGTGACTGACA
TTATGAATGATGCTCAGCGCAGTGAATTTGAAGATAAACTAGAAGTCGATTTTTCATTTGAGTTACCTAATATTGGACGC
TTTCGTGTAAATGCATTTAACCAGTCTCGTGGCTGTGCTGCTGTGTTTCGAACTATCCCTGTTGATATTCCGTCATTAGA
GCAATTAGCTGCACCTCAAGTGTTCACCGATATTGCAAAATATCAAAAAGGATTAGTGTTAATCACAGGGCCTACTGGTT
CAGGTAAGTCGACAACCTTAGCTGCTATGGTTGATTACATTAACGAGAACTACAACAAGCACGTACTTACGATTGAAGAT
CCGATTGAATTTGTTCATAAGAATAAGAAGTGTTTGATTAACCAACGTGAAGTTAATCGTGATACACATAGCTTTAAAGC
AGCACTTCGCTCAGCTTTGCGTGAAGATCCAGATGTTATTTTAGTCGGTGAATTACGTGATCAAGAGACAATCAGTCTTG
CATTGACCGCTGCTGAAACTGGGCATTTAGTATTTGGTACTTTGCACACGAGTTCAGCTGCAAAAACCATTGACCGTATT
ATTGATGTTTTCCCTGGCAGTGATAAATCTATGGTTCGCTCGATGTTGTCTGAATCATTAAGAGCGGTAGTAGCACAAAC
ACTGCTTAAACGTATTGGTGGTGGTCGTATTGCGTGTCATGAAATCATGATAGCGACACCAGCTATCCGTAACTTAATCC
GTGAAGATAAAGTCGCTCAAATGTATTCAATCATTCAAACTGGTTCTTCTTTTGGTATGAATACCATGGAGCAGAATGCG
AAGAAAATTATTGCTCAAGGTTTGGTTGATCCGGATGAAGTCGTTAAGAAAGTTGAAATGAGCAGCATAGGGTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

82.029

100

0.82
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

82.029

100

0.82
  pilT Acinetobacter baumannii strain A118

72.205

95.942

0.693
  pilT Acinetobacter baumannii D1279779

72.205

95.942

0.693
  pilT Acinetobacter nosocomialis M2

71.903

95.942

0.69
  pilT Pseudomonas stutzeri DSM 10701

68.732

98.261

0.675
  pilT Acinetobacter baylyi ADP1

70.303

95.652

0.672
  pilT Pseudomonas aeruginosa PAK

67.647

98.551

0.667
  pilT Legionella pneumophila strain Lp02

67.781

95.362

0.646
  pilT Legionella pneumophila strain ERS1305867

67.781

95.362

0.646
  pilT Neisseria meningitidis 8013

62.865

99.13

0.623
  pilT Neisseria gonorrhoeae MS11

62.573

99.13

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.968

98.261

0.481
  pilU Pseudomonas stutzeri DSM 10701

41.867

96.232

0.403
  pilU Vibrio cholerae strain A1552

40.615

94.203

0.383

Multiple sequence alignment