Detailed information NTG0001178

Experimentally validated

Overview

• Name: ideA
• Type: Regulator
• Locus tag: HJ37_RS00640
• Genome accession: NC_016445
• Coordinates: 152890..153573 (-)
• Length: 227 a.a.
• NCBI ID: WP_001082313.1
• Uniprot ID: G9IDB0
• Organism: Vibrio cholerae O1 str. 2010EL-1786
• Function: repress natural transformation Competence regulation

Function

An integrating conjugative element (ICE)-encoded DNase, which we name IdeA, is necessary and sufficient for inhibiting natural transformation of Haiti outbreak strains. IdeA inhibits this mechanism of HGT in cis via DNA endonuclease activity that is localized to the periplasm. Natural transformation between cholera strains in a relevant environmental context is inhibited by IdeA.

Genomic Context

Location: 147890..158573

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HJ37_RS00625 (Vch1786_I0125)	istB	149291..150046 (-)	756	WP_000546748.1	IS21-like element helper ATPase IstB	-
HJ37_RS00630 (Vch1786_I0126)	istA	150036..151535 (-)	1500	WP_001920874.1	IS21 family transposase	-
HJ37_RS00635 (Vch1786_I0127)	-	151643..152659 (-)	1017	WP_001918293.1	AAA family ATPase	-
HJ37_RS00640 (Vch1786_I0128)	ideA	152890..153573 (-)	684	WP_001082313.1	endonuclease	Regulator
HJ37_RS00645 (Vch1786_I0129)	-	153682..154284 (-)	603	WP_000035785.1	hypothetical protein	-
HJ37_RS00650 (Vch1786_I0130)	-	154652..154978 (+)	327	WP_000455723.1	hypothetical protein	-
HJ37_RS00655 (Vch1786_I0131)	-	154994..155413 (+)	420	WP_000795985.1	single-stranded DNA-binding protein	-
HJ37_RS00660 (Vch1786_I0132)	bet	155493..156311 (+)	819	WP_000414662.1	phage recombination protein Bet	-
HJ37_RS20140 (Vch1786_I0133)	-	156393..156536 (+)	144	WP_000167277.1	hypothetical protein	-
HJ37_RS00665 (Vch1786_I0134)	-	156597..157613 (+)	1017	WP_000862956.1	YqaJ viral recombinase family protein	-

Sequence

Protein

• Length: 227 a.a.
• Molecular weight: 26424.51 Da
• Isoelectric Point: 7.5402

>NTDB_id=1178 HJ37_RS00640 WP_001082313.1 152890..153573(-) (ideA) [Vibrio cholerae O1 str. 2010EL-1786]
MNRTNFLVTFLIALFAIPAFAEHPTSFSQAKRFAREIYQDNQSTFYCGCSYNNDGAIDAASCGYEPRKQPKRGERLEWEH
VVSAWEIGHQRQCWQNGGRRNCEKNDPEFSKMVSDLHNLVPSVGELNGDRSNFRFGMIPNESRAYGQCDFEVDFKDRRAE
PPANRQGDIARIYFYMRDQYGLRLSRQQTQLFEAWSRMDPVDEWEKLRDLRIRGIQGKSNCYVSGSC

Nucleotide

• Length: 684 bp

>NTDB_id=1178 HJ37_RS00640 WP_001082313.1 152890..153573(-) (ideA) [Vibrio cholerae O1 str. 2010EL-1786]
ATGAATAGAACTAATTTTCTCGTTACCTTCTTAATAGCGTTGTTCGCTATCCCTGCATTTGCAGAACACCCAACATCGTT
CAGCCAGGCAAAACGATTTGCCCGAGAAATTTACCAAGACAACCAGAGTACGTTTTACTGTGGATGTAGCTATAACAATG
ATGGTGCGATTGATGCTGCATCTTGCGGATATGAACCAAGGAAGCAACCGAAACGAGGAGAACGCTTAGAGTGGGAGCAC
GTTGTCTCAGCTTGGGAAATTGGCCATCAACGCCAATGCTGGCAAAACGGTGGACGTCGGAACTGCGAAAAGAATGATCC
TGAGTTTTCTAAAATGGTTTCGGATCTCCATAACCTCGTACCATCAGTAGGTGAGCTCAACGGGGATAGATCAAATTTTC
GATTTGGAATGATTCCGAACGAATCAAGGGCCTATGGTCAATGTGATTTCGAAGTTGATTTCAAAGACCGCCGAGCAGAA
CCACCAGCTAACCGTCAGGGTGATATTGCTAGAATTTATTTCTACATGCGAGATCAATACGGCCTAAGACTAAGCAGACA
ACAAACTCAGCTATTTGAAGCTTGGTCAAGAATGGACCCTGTTGATGAGTGGGAAAAATTACGTGATTTGAGGATTAGAG
GCATTCAAGGTAAGTCTAATTGCTATGTATCAGGCAGCTGCTAG

Domains

Predicted by InterProScan.

• Endonuclease I (Endonuclease_1) (41-224)

3D structure

• AlphaFold DB: G9IDB0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dns	Aliivibrio fischeri ES114	52.609	100	0.533
dns	Vibrio parahaemolyticus RIMD 2210633	52.402	100	0.529
dns	Vibrio cholerae strain A1552	51.556	99.119	0.511
dns	Campylobacter jejuni RM1221	38.393	100	0.386

References

• [1]: Ankur B Dalia et al. (2015) A globally distributed mobile genetic element inhibits natural transformation of Vibrio cholerae. Proceedings of The National Academy of Sciences of The United States of America 112(33):10485-90. [PMID: 26240317]