Detailed information    

experimental Experimentally validated

Overview

Name   dns   Type   Auxiliary factor
Locus tag   CJE_RS01260 Genome accession   NC_003912
Coordinates   233338..234009 (+) Length   223 a.a.
NCBI ID   WP_002876312.1    Uniprot ID   E5F1R4
Organism   Campylobacter jejuni RM1221     
Function   degradation of extracellular DNA   
Defense system

Function

Analysis of CJIE1 indicated the presence of dns (CJE0256), which is annotated as a gene encoding an extracellular DNase. Analysis of a broader set of strains indicated that the majority of nonnaturally transformable strains expressed DNase activity, while all naturally competent strains lacked this activity. The inhibition of natural transformation in C. jejuni via endogenous DNase activity may contribute to the formation of stable lineages in the C. jejuni population.


Genomic Context

Location: 228338..239009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJE_RS01230 (CJE0250) - 228698..230068 (+) 1371 WP_011049634.1 phage portal protein family protein -
  CJE_RS01235 (CJE0251) - 230070..231308 (+) 1239 WP_002888039.1 phage minor head protein -
  CJE_RS01240 (CJE0252) - 231434..231808 (+) 375 WP_002784519.1 phage tail protein -
  CJE_RS01245 (CJE0253) - 231801..231992 (+) 192 WP_002784520.1 tail protein X -
  CJE_RS01250 (CJE0254) - 231986..232963 (+) 978 WP_002784521.1 phage late control D family protein -
  CJE_RS01255 (CJE0255) - 232960..233244 (-) 285 WP_002784523.1 Mor transcription activator family protein -
  CJE_RS01260 (CJE0256) dns 233338..234009 (+) 672 WP_002876312.1 endonuclease Auxiliary factor
  CJE_RS01265 (CJE0257) - 234001..234276 (-) 276 WP_002784526.1 hypothetical protein -
  CJE_RS01270 (CJE0258) - 234323..234772 (-) 450 WP_002876315.1 regulatory protein GemA -
  CJE_RS01275 (CJE0259) - 234769..235461 (-) 693 WP_002793908.1 hypothetical protein -
  CJE_RS01280 (CJE0260) - 235461..235730 (-) 270 WP_002784531.1 hypothetical protein -
  CJE_RS01285 (CJE0261) - 235727..236689 (-) 963 WP_011049635.1 hypothetical protein -
  CJE_RS01290 (CJE0262) - 236798..237184 (-) 387 WP_002791413.1 YopX family protein -
  CJE_RS01295 (CJE0263) - 237181..237453 (-) 273 WP_002791414.1 hypothetical protein -
  CJE_RS01300 (CJE0264) - 237517..237696 (-) 180 WP_002791416.1 hypothetical protein -
  CJE_RS01305 (CJE0265) - 237693..238178 (-) 486 WP_002806832.1 host-nuclease inhibitor Gam family protein -
  CJE_RS01310 (CJE0266) - 238285..238626 (-) 342 WP_011049636.1 DUF4406 domain-containing protein -
  CJE_RS01315 (CJE0267) - 238722..238907 (-) 186 WP_011049637.1 hypothetical protein -

Sequence

Protein


Download         Length: 223 a.a.        Molecular weight: 26408.52 Da        Isoelectric Point: 10.0695

>NTDB_id=1237 CJE_RS01260 WP_002876312.1 233338..234009(+) (dns) [Campylobacter jejuni RM1221]
MKKIISVLILALSLLNAKSFEESKKELVKFYNDLGSSYWYDFYCQAPFKVNKKGKYISFEVIKSDLYAPRNEYTKKGKIN
QRIKRIEWEHIMPAQNFGKHLPCWKEGGRKACKNDPTFAKMEADKQNLVPAIGEINGDRSNFRYAEAPTNLKYTQYGNCK
VYTDFKAKRFYPANYSKGWIARSYLYMSKTYNIRLSDQERKLMEAWDKQYPMDEKEKRIRALL

Nucleotide


Download         Length: 672 bp        

>NTDB_id=1237 CJE_RS01260 WP_002876312.1 233338..234009(+) (dns) [Campylobacter jejuni RM1221]
ATGAAAAAAATAATAAGCGTTTTAATACTTGCTTTAAGCTTATTAAATGCTAAAAGTTTTGAAGAAAGCAAAAAAGAATT
AGTAAAATTTTATAATGATCTAGGGAGCTCTTACTGGTATGATTTTTATTGTCAAGCACCTTTTAAGGTTAATAAAAAAG
GAAAATATATTAGTTTTGAAGTGATTAAAAGTGATTTATATGCTCCTAGAAACGAATACACCAAAAAAGGAAAAATTAAC
CAAAGAATCAAACGCATAGAATGGGAGCATATTATGCCCGCCCAAAACTTTGGAAAGCATTTACCTTGCTGGAAAGAAGG
TGGCAGAAAAGCTTGTAAAAATGATCCAACTTTTGCAAAAATGGAAGCCGATAAACAAAACCTAGTTCCAGCCATAGGAG
AGATAAATGGGGATAGAAGCAATTTTAGATATGCTGAGGCTCCTACTAATTTAAAATATACTCAATATGGAAATTGTAAG
GTTTATACTGATTTTAAAGCAAAAAGATTTTATCCTGCAAATTATTCTAAAGGCTGGATTGCAAGAAGCTATTTATATAT
GAGCAAAACTTATAATATCAGATTATCCGACCAAGAAAGAAAACTTATGGAGGCTTGGGATAAACAATACCCTATGGATG
AGAAAGAAAAAAGAATTAGAGCATTACTCTAA

Domains

Predicted by InterproScan.

Endonuclease I (Endonuclease_1)

(21-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E5F1R4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dns Aliivibrio fischeri ES114

43.421

100

0.444
  dns Vibrio parahaemolyticus RIMD 2210633

42.534

99.103

0.422
  dns Vibrio cholerae strain A1552

39.45

97.758

0.386
  ideA Vibrio cholerae O1 str. 2010EL-1786

38.393

100

0.386

Multiple sequence alignment    



References

[1] Esther J Gaasbeek et al. (2009) A DNase encoded by integrated element CJIE1 inhibits natural transformation of Campylobacter jejuni. Journal of Bacteriology 191(7):2296-306. [PMID: 19151136]