Detailed information    

experimental Experimentally validated

Overview

Name   RA0C_RS07335   Type   Unclear
Locus tag   RA0C_RS07335 Genome accession   NC_017045
Coordinates   1542623..1544155 (+) Length   510 a.a.
NCBI ID   WP_004916218.1    Uniprot ID   E4TAR0
Organism   Riemerella anatipestifer ATCC 11845 = DSM 15868     
Function   require for natural transformation   
Unclear

Function

RA0C_RS09855, RA0C_RS09870, RA0C_RS09875, RA0C_RS09880, RA0C_RS03780, RA0C_RS07335, RA0C_RS08100, RA0C_RS04070, RA0C_RS07455, RA0C_RS05975, RA0C_RS06070, and RA0C_RS08490 significantly affected the natural transformation frequency of RA.


Genomic Context

Location: 1537623..1549155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA0C_RS07310 (RA0C_1527) - 1537665..1539125 (+) 1461 WP_013447036.1 SGNH/GDSL hydrolase family protein -
  RA0C_RS07315 (RA0C_1528) - 1539215..1540153 (+) 939 WP_004916214.1 ribose-phosphate pyrophosphokinase -
  RA0C_RS07320 (RA0C_1529) - 1540210..1540818 (+) 609 WP_004916215.1 50S ribosomal protein L25/general stress protein Ctc -
  RA0C_RS07325 (RA0C_1530) - 1540899..1541477 (+) 579 WP_004916216.1 SprT-like domain-containing protein -
  RA0C_RS07330 (RA0C_1531) - 1541491..1542579 (+) 1089 WP_013447038.1 mannose-1-phosphate guanylyltransferase -
  RA0C_RS07335 (RA0C_1532) RA0C_RS07335 1542623..1544155 (+) 1533 WP_004916218.1 YifB family Mg chelatase-like AAA ATPase Unclear
  RA0C_RS07340 (RA0C_1533) - 1544171..1544962 (+) 792 WP_004916219.1 GLPGLI family protein -
  RA0C_RS07345 (RA0C_1534) - 1545180..1545770 (+) 591 WP_004916220.1 RNA polymerase sigma factor -
  RA0C_RS10625 (RA0C_1535) - 1545818..1545952 (+) 135 WP_004916221.1 hypothetical protein -
  RA0C_RS07350 (RA0C_1536) - 1545968..1546663 (+) 696 WP_004916222.1 HAD family hydrolase -
  RA0C_RS07355 (RA0C_1537) trpS 1546761..1547729 (+) 969 WP_004916223.1 tryptophan--tRNA ligase -
  RA0C_RS07360 (RA0C_1538) - 1547787..1548473 (+) 687 WP_004916226.1 LysE family translocator -

Sequence

Protein


Download         Length: 510 a.a.        Molecular weight: 56731.24 Da        Isoelectric Point: 6.6997

>NTDB_id=1323 RA0C_RS07335 WP_004916218.1 1542623..1544155(+) (RA0C_RS07335) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MLVKVYGSAIHGVSAQTITIEVNVDQGVGYHLVGLPDNAIKESSHRISAALKNVGYKLPGKKITINMAPADLRKEGSAYD
LSIALGILAASGQIIAPEIERYLIMGELSLDGGLQPIKGVLPIAIRAREEGFKGIILPKQNTREAAIVNDLEVYGVENIK
EVIDFFNENCPLEPTKVNTREEFHKRVNLFPFDFSEVKGQETAKRAMEVAAAGGHNIILIGPPGSGKTMLAKRIPSILPP
LTLKEALETTKIHSVAGKMGAETSLMTIRPFRSPHHTISDVALVGGGSYPQPGEISLAHNGVLFLDEMPEFKRTVLEVMR
QPLEDREVTISRAKFTVNYPASFMLVASMNPSPSGFFPDDPNNTSSSFEMQRYLNKLSGPLLDRIDIHIEVQKVEFDQLS
DKRKGESSEIIRQRVLKAREIQQERYQDLAISYNAQMGPKEIEHFCELDEVSLLLIKNAMEKLNLSARAYDRILKVSRTI
ADLERETNIQSHHIAEAIQYRSLDRDFWKV

Nucleotide


Download         Length: 1533 bp        

>NTDB_id=1323 RA0C_RS07335 WP_004916218.1 1542623..1544155(+) (RA0C_RS07335) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGTTGGTAAAAGTTTACGGTAGTGCAATACATGGTGTTTCAGCACAAACTATCACAATAGAAGTTAATGTAGATCAAGG
TGTCGGTTACCATTTGGTAGGATTGCCAGACAATGCTATTAAAGAAAGTAGTCATAGAATTTCCGCTGCACTTAAAAATG
TAGGCTATAAATTGCCTGGGAAGAAAATTACCATAAATATGGCTCCTGCTGATCTCAGAAAAGAAGGTTCAGCCTATGAT
TTAAGCATTGCGTTGGGGATTTTAGCTGCATCGGGTCAGATTATAGCTCCAGAGATAGAGCGGTATCTTATTATGGGAGA
GCTTTCGTTAGATGGAGGTTTACAACCAATTAAAGGAGTGTTACCCATTGCAATAAGAGCTAGGGAAGAAGGTTTTAAAG
GAATTATTTTACCAAAACAAAACACAAGAGAGGCTGCTATTGTAAACGATTTGGAAGTTTATGGTGTAGAAAACATCAAA
GAGGTAATAGATTTTTTTAATGAAAACTGCCCTTTAGAACCAACTAAAGTCAATACACGAGAGGAATTTCACAAAAGAGT
AAATCTTTTTCCATTTGACTTTTCAGAAGTTAAAGGTCAAGAGACCGCTAAAAGAGCGATGGAAGTTGCGGCGGCAGGAG
GACATAATATTATTCTCATTGGTCCGCCAGGAAGTGGTAAAACAATGCTAGCAAAACGAATTCCAAGTATTCTTCCTCCT
TTAACATTGAAAGAAGCGTTAGAAACCACTAAAATACATTCCGTAGCAGGAAAGATGGGAGCTGAAACTTCCCTTATGAC
CATCAGACCTTTTAGGTCGCCGCATCATACTATTTCCGATGTAGCATTAGTAGGTGGCGGCAGTTATCCTCAACCTGGGG
AAATTTCTTTGGCTCACAATGGGGTGTTATTTTTAGATGAGATGCCAGAGTTTAAAAGAACTGTTCTAGAAGTTATGAGG
CAACCTTTGGAGGATAGAGAAGTTACTATTTCTAGAGCCAAGTTTACGGTAAACTATCCTGCTAGTTTTATGCTCGTGGC
ATCTATGAATCCTAGCCCTAGCGGATTTTTCCCTGATGACCCTAATAATACCTCTTCTTCTTTTGAGATGCAACGCTATC
TTAATAAACTTTCAGGACCTCTTTTGGACAGGATAGACATTCATATAGAAGTTCAAAAAGTGGAATTTGACCAACTATCC
GACAAAAGAAAAGGAGAGTCTAGTGAGATTATCAGACAAAGGGTGCTAAAAGCAAGGGAAATACAGCAAGAACGCTATCA
AGATTTGGCTATTAGTTACAATGCTCAGATGGGACCTAAAGAAATAGAGCATTTTTGCGAATTAGATGAGGTATCTCTAC
TTTTAATCAAAAATGCTATGGAAAAGCTCAATCTTTCTGCAAGGGCTTATGACCGTATTTTAAAAGTATCTAGAACCATA
GCAGATTTAGAAAGAGAAACCAATATACAAAGTCATCACATTGCGGAAGCTATACAGTATAGAAGTTTAGATAGAGATTT
TTGGAAAGTTTAA

Domains

Predicted by InterproScan.

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(193-398)

Subunit ChlI of Mg-chelatase (ChlI)

(20-141)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(405-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4TAR0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

46.796

100

0.473
  comM Glaesserella parasuis strain SC1401

46.784

100

0.471
  comM Vibrio campbellii strain DS40M4

43.701

100

0.438
  comM Vibrio cholerae strain A1552

43.222

100

0.434
  comM Legionella pneumophila str. Paris

42.339

98.608

0.417
  comM Legionella pneumophila strain ERS1305867

42.339

98.608

0.417

Multiple sequence alignment    



References

[1] Li Huang et al. (2021) Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis. Frontiers in Microbiology 12:712198. [PMID: 34566918]