Detailed information NTG0001387

Experimentally validated

Overview

• Name: comM
• Type: Unclear
• Locus tag: A4U84_RS06900
• Genome accession: NZ_CP015099
• Coordinates: 1464459..1465991 (+)
• Length: 510 a.a.
• NCBI ID: WP_020457569.1
• Uniprot ID: -
• Organism: Glaesserella parasuis strain SC1401
• Function: require for natural transformation Unclear

Function

The expression levels of candidate uptake components were significantly lower than in SC1401, respectively. These results revealed that the highly naturally competent strain SC1401 up-regulated most of the candidate natural competence-related regulons/components.

Genomic Context

Location: 1459459..1470991

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A4U84_RS06875 (A4U84_06875)	-	1461611..1462783 (-)	1173	WP_062924215.1	FAD-dependent oxidoreductase	-
A4U84_RS06880 (A4U84_06880)	-	1462996..1463301 (+)	306	WP_021111771.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A4U84_RS06885 (A4U84_06885)	-	1463308..1463613 (+)	306	WP_005713249.1	addiction module antidote protein	-
A4U84_RS06890 (A4U84_06890)	-	1463755..1464030 (+)	276	WP_005713247.1	type II toxin-antitoxin system HicA family toxin	-
A4U84_RS06895 (A4U84_06895)	-	1464008..1464367 (+)	360	WP_021113001.1	type II toxin-antitoxin system HicB family antitoxin	-
A4U84_RS06900 (A4U84_06900)	comM	1464459..1465991 (+)	1533	WP_020457569.1	YifB family Mg chelatase-like AAA ATPase	Unclear
A4U84_RS06905 (A4U84_06905)	-	1465988..1466977 (-)	990	WP_081120226.1	DNA polymerase III subunit delta'	-
A4U84_RS06910 (A4U84_06910)	-	1466992..1467204 (-)	213	WP_010786558.1	BrnA antitoxin family protein	-
A4U84_RS06915 (A4U84_06915)	-	1467201..1467488 (-)	288	WP_010786559.1	BrnT family toxin	-
A4U84_RS06920 (A4U84_06920)	tmk	1467543..1468169 (-)	627	WP_021111765.1	dTMP kinase	-
A4U84_RS06925 (A4U84_06925)	mltG	1468169..1469203 (-)	1035	WP_062924217.1	endolytic transglycosylase MltG	-
A4U84_RS06945 (A4U84_06945)	tpiA	1469754..1470545 (-)	792	WP_062924218.1	triose-phosphate isomerase	-

Sequence

Protein

• Length: 510 a.a.
• Molecular weight: 55941.10 Da
• Isoelectric Point: 8.6558

>NTDB_id=1387 A4U84_RS06900 WP_020457569.1 1464459..1465991(+) (comM) [Glaesserella parasuis strain SC1401]
MSLAIIYSRASIGVEAPLVTIEVHISGGSPGLTLVGLPEKSVKEAQDRVRSALMNANFDYPARRITINLAPADLPKEGGR
FDLPIAIGILAASGQIDSDKLKRIELLGELSLTGHLRGVHGAIPAVLAASKAKREIIVPQQNANEVSLVSNTQTYCASTL
LQVVNFLNNRDQLPIAQQLSQNIAENRPLVSRDLTDIIGQQHAKRALIIAASGQHNLLFLGPPGTGKTMLASRLADLLPE
MEDDEAIETASVTSLVQNELNFQNWKKRPFRSPHHSASMVALVGGGSIPKPGEISLAHNGVLFLDELPEFERKVLDALRQ
PLESGEIIISRANAKVQFPACFQLIAAMNPSPTGHYQGTHNRTSPQQLMRYLNRLSGPFLDRFDLSIEVPLLPQGALQNN
TDQRGETTEQARKRVLEARHLQFNRRGKINAQLTTKEIEQDCKLTDRDALFLENALTKLGLSVRAYHRILKVSRTIADLA
GEAQIQQPHLAEALGYRAMDRLLQRLQGES

Nucleotide

• Length: 1533 bp

>NTDB_id=1387 A4U84_RS06900 WP_020457569.1 1464459..1465991(+) (comM) [Glaesserella parasuis strain SC1401]
ATGTCTTTAGCGATTATTTACAGTCGAGCATCTATCGGTGTTGAAGCCCCATTAGTAACCATTGAAGTGCACATCAGTGG
CGGAAGCCCTGGACTTACCCTAGTTGGATTGCCCGAAAAAAGCGTCAAAGAAGCACAGGATAGGGTGCGAAGTGCCTTGA
TGAATGCCAATTTTGATTACCCAGCCCGACGCATTACCATCAACCTTGCCCCAGCCGATCTCCCCAAAGAAGGCGGACGT
TTTGATTTACCCATTGCCATCGGGATTTTGGCAGCATCGGGGCAGATTGACAGCGATAAACTCAAACGGATTGAACTGCT
TGGCGAGCTGTCTTTGACGGGGCATTTGCGTGGGGTTCACGGTGCTATTCCTGCGGTGTTGGCAGCAAGTAAAGCGAAAC
GGGAAATCATCGTGCCACAACAAAACGCCAATGAAGTTTCCTTGGTTTCCAATACCCAAACCTATTGTGCTTCAACCTTG
TTGCAGGTTGTCAATTTTCTCAACAATCGAGATCAACTGCCGATTGCTCAGCAGCTTTCGCAAAATATTGCCGAAAACAG
ACCGCTTGTCAGCCGTGATTTAACCGATATTATCGGGCAACAACACGCCAAACGGGCATTGATTATTGCCGCCAGCGGTC
AGCATAATCTGTTGTTTTTAGGTCCACCTGGTACAGGCAAAACAATGCTTGCTAGTCGCCTTGCCGATCTGCTGCCTGAA
ATGGAAGATGATGAAGCAATTGAAACAGCATCTGTCACCAGTCTTGTACAAAATGAACTCAACTTTCAGAACTGGAAAAA
ACGCCCGTTTCGTTCCCCACATCACAGTGCATCAATGGTTGCGTTGGTTGGGGGCGGTTCGATCCCGAAACCAGGGGAAA
TTTCACTGGCACACAACGGTGTGCTGTTTTTAGACGAATTGCCTGAATTTGAACGGAAAGTGTTAGATGCATTACGCCAG
CCGTTGGAGTCGGGAGAAATCATTATTTCCAGAGCCAATGCCAAGGTGCAGTTTCCTGCTTGTTTTCAACTGATTGCCGC
AATGAACCCAAGCCCGACAGGGCATTATCAAGGAACGCACAATCGAACGTCACCACAACAGTTAATGCGTTATCTCAACC
GTTTATCAGGGCCTTTTCTCGACCGCTTCGATCTCTCGATTGAAGTGCCGTTACTGCCACAAGGTGCATTGCAAAATAAC
ACCGATCAACGTGGCGAAACCACCGAACAGGCTCGCAAGCGGGTACTTGAAGCAAGACATTTGCAATTTAACCGTCGGGG
GAAAATCAATGCTCAACTGACGACCAAAGAGATTGAACAAGATTGCAAACTGACTGATCGAGATGCGTTATTTTTAGAAA
ATGCCTTAACGAAACTGGGGCTGTCGGTGCGAGCTTATCACCGTATTCTCAAAGTTTCTCGCACCATTGCTGACTTGGCT
GGAGAAGCCCAAATCCAACAGCCCCATCTGGCTGAAGCTTTGGGCTATCGGGCGATGGATCGTTTGTTACAAAGGTTACA
AGGGGAAAGTTAG

Domains

Predicted by InterproScan.

• Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C) (405-497)

• Magnesium chelatase, subunit ChlI (Mg_chelatase) (193-394)

• Subunit ChlI of Mg-chelatase (ChlI) (21-142)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Haemophilus influenzae Rd KW20	78.895	99.607	0.786
comM	Vibrio cholerae strain A1552	64.147	100	0.653
comM	Vibrio campbellii strain DS40M4	63.922	100	0.643
comM	Legionella pneumophila strain ERS1305867	48.207	99.801	0.481
comM	Legionella pneumophila str. Paris	48.207	99.801	0.481
RA0C_RS07335	Riemerella anatipestifer ATCC 11845 = DSM 15868	46.784	100	0.471

References

• [1]: Ke Dai et al. (2018) Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401. Frontiers in Cellular And Infection Microbiology 8:32. [PMID: 29473023]