Detailed information    

experimental Experimentally validated

Overview

Name   comM   Type   Machinery gene
Locus tag   GCO85_RS03150 Genome accession   NZ_CP048618
Coordinates   658085..659596 (-) Length   503 a.a.
NCBI ID   WP_011213198.1    Uniprot ID   A0A3A6V972
Organism   Legionella pneumophila strain ERS1305867     
Function   DNA binding   
DNA binding and uptake

Function

Mutation of this gene caused a decrease in transformation.


Genomic Context

Location: 653085..664596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS03120 (GCO85_03130) - 653629..654300 (-) 672 WP_011213193.1 TIGR00153 family protein -
  GCO85_RS03125 (GCO85_03135) - 654491..655255 (+) 765 WP_011213194.1 hypothetical protein -
  GCO85_RS03130 (GCO85_03140) - 655558..656121 (+) 564 WP_010946323.1 YqgE/AlgH family protein -
  GCO85_RS03135 (GCO85_03145) ruvX 656114..656533 (+) 420 WP_011213195.1 Holliday junction resolvase RuvX -
  GCO85_RS03140 (GCO85_03150) - 656583..657476 (+) 894 WP_011213196.1 aspartate carbamoyltransferase catalytic subunit -
  GCO85_RS03145 (GCO85_03155) - 657590..657973 (-) 384 WP_014841085.1 hypothetical protein -
  GCO85_RS03150 (GCO85_03160) comM 658085..659596 (-) 1512 WP_011213198.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  GCO85_RS03155 (GCO85_03165) - 659661..659921 (-) 261 WP_011213199.1 accessory factor UbiK family protein -
  GCO85_RS03160 (GCO85_03170) - 660066..660404 (+) 339 WP_011213200.1 P-II family nitrogen regulator -
  GCO85_RS03165 (GCO85_03175) - 660414..660995 (-) 582 WP_014841086.1 5-formyltetrahydrofolate cyclo-ligase -
  GCO85_RS03170 (GCO85_03180) - 661242..661427 (+) 186 WP_010946331.1 hypothetical protein -
  GCO85_RS03175 (GCO85_03185) - 661441..662256 (+) 816 WP_011945758.1 aminotransferase class IV -
  GCO85_RS03180 (GCO85_03190) - 662283..662966 (-) 684 WP_050069630.1 TVP38/TMEM64 family protein -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 54951.54 Da        Isoelectric Point: 8.2499

>NTDB_id=1248 GCO85_RS03150 WP_011213198.1 658085..659596(-) (comM) [Legionella pneumophila strain ERS1305867]
MSLAFTKTRSTIGIVAQPVSVEVHLSNGLPSFTMVGLAETAVKESKDRVRSAIINSQFEFPCRKITVNLGPANLPKTGSG
FDLPIALGILAASEQIPLTNLANHEFIGELALSGELRGVSAIIPAVLAAHKDNQHLIIANANAAEASLTGHQKVFTANNL
REVCDYLCQGTSLQSLPPKPDLLLNNYELDWSDIKGQQHAKNAMVIAACGGHSILLSGAPGSGKTMMAKRFSTLLPELSE
TQALECAAINSIRGKLPDFREWRLPPFRAPHHTASPVALVGGGNPPKPGEISLAHHGVLFLDELPEFNRQVLETLREPLE
SGHICISRAAAQIEFPAKFQLIAAMNPCPCGQWGNSQANCMCTPDRISRYLAKLSAPLLDRIDMQVTIHALSQEELIKPN
THLEKQSLAIREKVTKMHEIQMARQDSLNANLNSKTCEMVCELGSEEQLFLREVMSKLKLSARGYHRLLKVSRTIADMNS
SKKVLLNHLQQALSYKQNLHLPK

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=1248 GCO85_RS03150 WP_011213198.1 658085..659596(-) (comM) [Legionella pneumophila strain ERS1305867]
ATGAGTCTCGCATTTACCAAAACGCGAAGTACTATTGGTATTGTCGCACAACCAGTATCTGTGGAAGTCCATTTATCAAA
TGGATTGCCAAGTTTTACTATGGTAGGACTTGCTGAAACAGCAGTGAAAGAAAGTAAAGACCGCGTGCGCAGCGCCATTA
TAAATAGCCAATTCGAATTTCCATGCCGTAAAATTACCGTTAATCTTGGCCCTGCCAATTTACCTAAAACGGGAAGTGGC
TTTGACTTGCCTATTGCACTAGGGATACTCGCAGCATCAGAACAAATTCCTCTCACCAACCTTGCAAACCACGAATTTAT
TGGAGAATTGGCTTTAAGCGGCGAGCTTAGAGGAGTTTCTGCCATTATACCGGCTGTTCTTGCCGCTCATAAAGACAATC
AACATCTGATTATAGCGAATGCCAATGCTGCCGAAGCTTCTTTAACCGGCCATCAAAAAGTCTTTACTGCCAATAACTTA
CGTGAAGTTTGTGATTATCTATGCCAGGGCACTTCTTTACAAAGCTTACCTCCCAAACCAGATCTTTTATTAAATAACTA
TGAATTGGATTGGTCTGATATTAAGGGACAACAGCATGCCAAAAATGCTATGGTCATCGCTGCCTGTGGGGGACACAGCA
TTCTGCTAAGTGGCGCACCAGGAAGTGGAAAAACGATGATGGCAAAAAGATTTAGCACCTTGCTACCTGAGCTATCCGAA
ACACAAGCTCTCGAATGCGCAGCCATTAATTCAATTCGAGGCAAATTACCTGATTTCAGGGAATGGCGTTTGCCTCCTTT
TCGTGCACCACATCATACTGCTTCACCTGTCGCGTTAGTCGGAGGAGGCAACCCGCCGAAACCTGGTGAAATTTCATTAG
CCCATCACGGCGTATTATTTTTAGATGAATTACCTGAATTTAATCGGCAAGTATTGGAGACACTTAGAGAACCTTTGGAG
TCAGGGCATATTTGCATTTCCAGGGCAGCCGCACAAATAGAGTTTCCTGCCAAATTTCAATTAATTGCAGCAATGAATCC
TTGCCCTTGTGGCCAATGGGGTAATTCACAAGCCAATTGCATGTGTACTCCTGATAGAATCAGCCGCTATTTGGCAAAAC
TTTCTGCTCCATTATTAGATAGAATCGATATGCAGGTTACAATTCACGCATTATCTCAAGAGGAGTTAATTAAACCCAAT
ACCCATTTAGAAAAACAAAGTCTCGCTATCAGAGAAAAGGTAACAAAGATGCATGAAATACAAATGGCACGACAAGATAG
TCTCAATGCCAATTTAAATTCCAAAACATGTGAAATGGTTTGCGAATTGGGATCAGAAGAACAATTGTTTCTCCGAGAAG
TGATGAGTAAACTGAAACTGTCTGCTCGTGGCTACCATCGCTTACTAAAAGTAAGCAGGACAATTGCTGATATGAATAGC
AGTAAGAAAGTTCTGTTAAATCACTTGCAACAAGCTTTATCTTACAAACAAAATTTACACTTACCGAAATGA

Domains

Predicted by InterproScan.

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(190-395)

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(405-496)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8RXD
  PDB 8RXK
  PDB 8RXS
  PDB 8RXT

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Legionella pneumophila str. Paris

100

100

1
  comM Haemophilus influenzae Rd KW20

50.787

100

0.513
  comM Vibrio cholerae strain A1552

50.806

98.608

0.501
  comM Vibrio campbellii strain DS40M4

50.201

99.006

0.497
  comM Glaesserella parasuis strain SC1401

48.207

99.801

0.481
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

42.339

98.608

0.417

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]