Detailed information    

experimental Experimentally validated

Overview

Name   comM   Type   Machinery gene
Locus tag   HI_1117 Genome accession   L42023
Coordinates   1183115..1184644 (-) Length   509 a.a.
NCBI ID   AAC22771.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   require for natural transformation   
Unclear

Function

The deletion of comM had an unexpectedly small effect. As previously reported for a comM transposon mutant, DNA uptake was normal, but transformation by our mutant was reduced only 6-fold rather than the 300-fold previously reported.


Genomic Context

Location: 1178115..1189644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_1113 - 1179231..1180766 (+) 1536 AAC22767.1 xylulose kinase -
  HI_1114 - 1180817..1181743 (-) 927 AAC22768.1 ADP-L-glycero-D-mannoheptose-6-epimerase (rfaD) -
  HI_1115 - 1181796..1182299 (-) 504 AAC22769.1 thioredoxin, putative -
  HI_1116 - 1182410..1183081 (+) 672 AAC22770.1 deoxyribose-phosphate aldolase (deoC) -
  HI_1117 comM 1183115..1184644 (-) 1530 AAC22771.1 competence protein (comM) Machinery gene
  HI_1118 - 1184759..1185376 (-) 618 AAC22772.1 conserved hypothetical GTP-binding protein -
  HI_1119 - 1185469..1186347 (+) 879 AAC22773.1 membrane protein (lapB) -
  HI_1120 - 1186387..1187385 (-) 999 AAC22774.1 oligopeptide ABC transporter, ATP-binding protein (oppF) -
  HI_1121 - 1187382..1188353 (-) 972 AAC22775.1 oligopeptide ABC transporter, ATP-binding protein (oppD) -
  HI_1122 - 1188363..1189298 (-) 936 AAC22776.1 oligopeptide ABC transporter, permease protein (oppC) -

Sequence

Protein


Download         Length: 509 a.a.        Molecular weight: 55915.49 Da        Isoelectric Point: 9.8671

>NTDB_id=1349 HI_1117 AAC22771.1 1183115..1184644(-) (comM) [Haemophilus influenzae Rd KW20]
MSLAIVYSRASMGVQAPLVTIEVHLSNGKPGFTLVGLPEKTVKEAQDRVRSALMNAQFKYPAKRITVNLAPADLPKEGGR
FDLPIAIGILAASDQLDASHLKQFEFVAELALTGQLRGVHGVIPAILAAQKSKRELIIAKQNANEASLVSDQNTYFAQTL
LDVVQFLNGQEKLPLATEIVKESAVNFSGKNTLDLTDIIGQQHAKRALTIAAAGQHNLLFLGPPGTGKTMLASRLTGLLP
EMTDLEAIETASVTSLVQNELNFHNWKQRPFRAPHHSASMPALVGGGTIPKPGEISLATNGVLFLDELPEFERKVLDALR
QPLESGEIIISRANAKIQFPARFQLVAAMNPSPTGHYTGTHNRTSPQQIMRYLNRLSGPFLDRFDLSIEVPLLPQGSLQN
TGDRGETSAQVREKVLKVREIQMERAGKINAYLNSKEIERDCKLNDKDAFFLEKALNKLGLSVRAYHRILKVSRTIADLQ
GEQQIFQPHLAEALGYRAMVRLLQKLSNM

Nucleotide


Download         Length: 1530 bp        

>NTDB_id=1349 HI_1117 AAC22771.1 1183115..1184644(-) (comM) [Haemophilus influenzae Rd KW20]
ATGTCCCTTGCTATTGTTTACAGCCGTGCCTCTATGGGCGTGCAAGCGCCGCTTGTCACCATTGAGGTGCATTTAAGCAA
CGGAAAACCAGGATTTACACTTGTTGGTTTGCCAGAAAAAACCGTGAAAGAGGCACAAGATAGGGTGCGTAGTGCGTTGA
TGAATGCACAATTTAAATACCCAGCCAAGCGTATCACTGTAAATCTTGCACCTGCGGATTTACCGAAAGAAGGCGGAAGA
TTTGATTTGCCTATCGCCATCGGAATTTTAGCCGCATCAGATCAGCTTGATGCGAGCCACTTAAAGCAATTTGAATTTGT
GGCAGAGCTTGCGCTGACGGGCCAATTGCGTGGCGTACATGGTGTGATTCCCGCTATTCTTGCAGCGCAAAAGTCAAAGC
GAGAGTTAATTATTGCGAAACAAAATGCTAATGAAGCCTCGCTCGTGTCTGATCAAAATACTTATTTTGCTCAAACGCTT
TTAGATGTAGTGCAATTTCTCAATGGTCAGGAAAAATTACCACTCGCCACTGAAATTGTGAAAGAAAGTGCGGTAAATTT
TTCAGGTAAAAATACGTTAGATTTAACGGATATTATCGGACAACAGCACGCTAAACGAGCATTGACCATTGCCGCAGCGG
GGCAGCATAATTTGCTCTTTCTTGGCCCACCGGGTACAGGGAAAACCATGTTAGCCAGCCGTTTGACAGGGCTTTTACCT
GAAATGACAGATTTAGAAGCGATAGAAACGGCATCTGTAACGAGTTTAGTTCAAAACGAGTTAAATTTTCATAATTGGAA
GCAACGTCCTTTTCGTGCACCACATCATAGTGCATCAATGCCTGCTTTAGTTGGTGGCGGAACGATCCCTAAACCTGGCG
AAATATCCTTAGCAACAAATGGGGTACTTTTTCTTGATGAACTTCCAGAATTTGAGCGAAAAGTGTTAGATGCACTACGT
CAGCCTTTGGAAAGTGGTGAGATTATTATTTCTCGTGCTAATGCCAAAATTCAATTTCCGGCTCGTTTTCAATTAGTGGC
AGCAATGAATCCAAGTCCGACAGGCCATTATACAGGAACACATAACCGCACTTCACCACAGCAAATTATGCGTTATTTGA
ATCGACTTTCAGGGCCATTTTTAGATCGTTTTGATTTATCTATTGAAGTGCCTTTACTGCCACAAGGTAGCTTGCAAAAT
ACGGGCGATCGTGGCGAAACCAGCGCGCAAGTTCGTGAAAAAGTGTTAAAAGTACGTGAGATTCAAATGGAAAGAGCGGG
GAAAATCAACGCTTATTTGAACAGTAAAGAGATTGAGCGTGATTGCAAGTTAAATGATAAAGATGCCTTTTTCCTTGAAA
AAGCACTGAATAAACTGGGGCTTTCTGTTCGGGCTTATCATCGTATTTTGAAAGTTTCTCGAACCATTGCCGATCTACAA
GGAGAACAACAAATTTTTCAACCGCACTTGGCGGAGGCCTTGGGCTATCGAGCAATGGTTCGTTTGCTGCAGAAATTGTC
GAATATGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(194-395)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(405-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Glaesserella parasuis strain SC1401

78.895

99.607

0.786
  comM Vibrio cholerae strain A1552

67.258

100

0.673
  comM Vibrio campbellii strain DS40M4

65.878

100

0.659
  comM Legionella pneumophila strain ERS1305867

50.787

100

0.513
  comM Legionella pneumophila str. Paris

50.787

100

0.513
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

46.796

100

0.473

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]
[2] M L Gwinn et al. (1998) A new transformation-deficient mutant of Haemophilus influenzae Rd with normal DNA uptake. Journal of Bacteriology 180(3):746-8. [PMID: 9457884]