Detailed information    

experimental Experimentally validated

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PSJM300_03940 Genome accession   CP003725
Coordinates   872824..873243 (-) Length   139 a.a.
NCBI ID   AFN76866.1    Uniprot ID   -
Organism   Pseudomonas stutzeri DSM 10701     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

Insertional inactivation of pilA abolished pilus formation, PO4 plating, twitching motility, and natural transformation.


Genomic Context

Location: 867824..878243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSJM300_03920 - 869533..869973 (-) 441 AFN76862.1 hypothetical protein -
  PSJM300_03925 - 870169..870918 (-) 750 AFN76863.1 type IV pilin accessory protein -
  PSJM300_03930 - 870915..872306 (-) 1392 AFN76864.1 pilus biogenesis protein -
  PSJM300_03935 pilA/pilAII 872331..872756 (-) 426 AFN76865.1 fimbrial protein pilin: general secretion pathway protein H Regulator
  PSJM300_03940 pilA/pilAI 872824..873243 (-) 420 AFN76866.1 fimbrial protein pilin: general secretion pathway protein H Machinery gene
  PSJM300_03945 pilB 873564..875267 (+) 1704 AFN76867.1 type 4 fimbrial biogenesis protein PilB Machinery gene
  PSJM300_03950 pilC 875269..876486 (+) 1218 AFN76868.1 type 4 pilus biogenesis protein PilC Machinery gene
  PSJM300_03955 pilD 876489..877358 (+) 870 AFN76869.1 type 4 prepilin peptidase PilD Machinery gene
  PSJM300_03960 coaE 877599..878207 (+) 609 AFN76870.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14558.49 Da        Isoelectric Point: 4.5141

>NTDB_id=1197 PSJM300_03940 AFN76866.1 872824..873243(-) (pilA/pilAI) [Pseudomonas stutzeri DSM 10701]
MKAQMQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRSNAAAALAEITPGKIGFEQAINEGKTPSLTSTDEGYIGITDS
TSYCDVDLDTAADGHIECTAKGGNAGKFDGKTITLNRTADGEWSCASTLDAKYKPGKCS

Nucleotide


Download         Length: 420 bp        

>NTDB_id=1197 PSJM300_03940 AFN76866.1 872824..873243(-) (pilA/pilAI) [Pseudomonas stutzeri DSM 10701]
ATGAAAGCCCAAATGCAGAAAGGTTTTACCCTGATTGAACTGATGATCGTAGTTGCGATTATCGGTATTTTGGCCGCCAT
CGCGCTGCCGGCATATCAAGATTACACCGTACGTTCCAACGCTGCCGCAGCCCTGGCCGAAATCACCCCCGGCAAAATCG
GTTTTGAGCAGGCGATCAACGAGGGTAAAACTCCGTCGCTCACTTCTACTGACGAAGGCTACATTGGCATTACCGACTCG
ACTTCTTACTGTGACGTAGATCTTGACACTGCTGCAGATGGTCACATCGAGTGCACTGCAAAAGGCGGCAATGCCGGCAA
GTTTGATGGCAAAACAATTACGCTTAACCGTACGGCTGATGGCGAATGGAGCTGCGCCAGCACCTTGGACGCCAAGTACA
AGCCAGGCAAGTGCTCCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)

Pilin (bacterial filament) (Pilin)

(37-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAII Pseudomonas stutzeri DSM 10701

67.424

94.964

0.64
  pilA Pseudomonas aeruginosa PAK

47.682

100

0.518
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.359

100

0.482
  comP Acinetobacter baylyi ADP1

43.448

100

0.453
  pilA Acinetobacter baumannii strain A118

45.985

98.561

0.453
  pilA/pilA1 Eikenella corrodens VA1

39.49

100

0.446
  pilA2 Legionella pneumophila str. Paris

42.963

99.265

0.426
  pilA2 Legionella pneumophila strain ERS1305867

42.963

99.265

0.426
  pilA Vibrio cholerae strain A1552

39.86

100

0.41
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.86

100

0.41
  pilA Vibrio cholerae C6706

39.86

100

0.41
  pilA Vibrio parahaemolyticus RIMD 2210633

35.862

100

0.374

Multiple sequence alignment    



References

[1] Petra Meier et al. (2002) Natural transformation of Pseudomonas stutzeri by single-stranded DNA requires type IV pili, competence state and comA. FEMS Microbiology Letters 207(1):75-80. [PMID: 11886754]
[2] S Graupner et al. (2001) Pseudomonas stutzeri has two closely related pilA genes (Type IV pilus structural protein) with opposite influences on natural genetic transformation. Journal of Bacteriology 183(7):2359-66. [PMID: 11244078]
[3] S Graupner et al. (2000) Type IV pilus genes pilA and pilC of Pseudomonas stutzeri are required for natural genetic transformation, and pilA can be replaced by corresponding genes from nontransformable species. Journal of Bacteriology 182(8):2184-90. [PMID: 10735861]