Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSJM300_03945 Genome accession   CP003725
Coordinates   873564..875267 (+) Length   567 a.a.
NCBI ID   AFN76867.1    Uniprot ID   -
Organism   Pseudomonas stutzeri DSM 10701     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 868564..880267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSJM300_03920 - 869533..869973 (-) 441 AFN76862.1 hypothetical protein -
  PSJM300_03925 - 870169..870918 (-) 750 AFN76863.1 type IV pilin accessory protein -
  PSJM300_03930 - 870915..872306 (-) 1392 AFN76864.1 pilus biogenesis protein -
  PSJM300_03935 pilA/pilAII 872331..872756 (-) 426 AFN76865.1 fimbrial protein pilin: general secretion pathway protein H Regulator
  PSJM300_03940 pilA/pilAI 872824..873243 (-) 420 AFN76866.1 fimbrial protein pilin: general secretion pathway protein H Machinery gene
  PSJM300_03945 pilB 873564..875267 (+) 1704 AFN76867.1 type 4 fimbrial biogenesis protein PilB Machinery gene
  PSJM300_03950 pilC 875269..876486 (+) 1218 AFN76868.1 type 4 pilus biogenesis protein PilC Machinery gene
  PSJM300_03955 pilD 876489..877358 (+) 870 AFN76869.1 type 4 prepilin peptidase PilD Machinery gene
  PSJM300_03960 coaE 877599..878207 (+) 609 AFN76870.1 dephospho-CoA kinase -
  PSJM300_03965 - 878204..878398 (+) 195 AFN76871.1 DNA gyrase inhibitor -
  PSJM300_03970 - 878730..879926 (+) 1197 AFN76872.1 FHA domain-containing protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61815.77 Da        Isoelectric Point: 5.3460

>NTDB_id=20260 PSJM300_03945 AFN76867.1 873564..875267(+) (pilB) [Pseudomonas stutzeri DSM 10701]
MSENISLSGLARQMVRAGVLDEKTAQQAQVQAQRNKVSLVTYVVQNKLAKGRAVAELASDQFGVALLDLSALDKDSLPKE
LVSEKLARQHRVLPLVKRGTKLYVAISDPANHQSITDIQFSTGLTIESILVEDDKLGDAIDKLYDSGTSGLEDLGDVDLD
GIDVESVSDDAKGGEEGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGVLHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKAKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEESQKQLYMDALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCSSCKKEVEVPRDALLKEGFP
EESIGTFKIYGPVGCENCVGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALIKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=20260 PSJM300_03945 AFN76867.1 873564..875267(+) (pilB) [Pseudomonas stutzeri DSM 10701]
ATGAGCGAAAACATTTCCCTTTCCGGCTTGGCTCGACAGATGGTGCGGGCTGGTGTGCTGGACGAGAAAACCGCGCAACA
GGCGCAAGTGCAGGCGCAGCGCAACAAGGTGTCGCTAGTTACCTATGTAGTGCAGAACAAGCTGGCGAAAGGGCGGGCGG
TTGCCGAGCTTGCGTCCGACCAGTTTGGGGTCGCACTACTGGATTTGTCTGCGCTGGACAAGGACTCGCTACCCAAAGAG
TTGGTGAGTGAGAAACTGGCGCGTCAGCACCGCGTGCTACCACTGGTCAAGCGCGGTACCAAGCTTTATGTCGCCATTTC
CGACCCGGCCAACCACCAGTCGATCACGGATATCCAGTTCAGCACCGGGCTGACCATCGAGTCGATCTTGGTGGAGGACG
ATAAGCTGGGCGACGCTATCGACAAGCTTTATGACAGCGGTACGAGTGGTTTGGAGGATTTAGGCGATGTCGATCTGGAC
GGCATCGACGTCGAGTCTGTCAGTGATGATGCCAAAGGCGGGGAGGAGGGTGAGGCGGCCGACGACGCCCCGGTAGTACG
CTTCGTCAATAAGATGCTGCTCGATGCCATACGCGGTGGCTCGTCGGACCTGCACTTTGAGCCCTACGAAAAGGCCTATC
GTGTGCGCCTGCGTACCGATGGGGTGCTGCATGAGGTGGCGCGGCCACCGATCCAGCTGGCTCCGCGCATCTCCGCGCGG
TTGAAGGTGATGGCTGGGCTGGATATTTCCGAGCGGCGCAAGCCGCAGGATGGCCGGATCAAGATGAAGCTTTCCAAGGC
CAAGGCCATCGACTTTCGCGTGAACACACTGCCGACGTTGTGGGGCGAAAAGATCGTGATGCGGATTCTCGACCCTTCCA
GCGCACAGATGGGTATCGATGCGCTGGGTTACGAGGAGTCGCAGAAGCAGCTGTATATGGATGCGCTCAAGCAGCCCCAG
GGCATGATTCTGGTCACGGGGCCGACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAACATTCTCAACACGGT
GGACGTGAATATCTCGACTGCCGAGGACCCGGTGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCC
AGGGTATGGACTTTGCCCAGGCATTGCGCGCCTTCCTGCGCCAGGACCCGGACGTAATCATGGTGGGCGAGATTCGTGAC
CTGGAAACGGCCGAGATCGCCATCAAGGCGGCGCAGACAGGGCATATGGTGATGTCCACTCTGCACACCAACAGCGCCGC
GGAAACCCTCACCCGCCTGCGCAACATGGGGGTGGCTGCATTCAATATCGCCACGTCGGTGAACCTGATCATCGCCCAGC
GCCTGGCACGCAAGCTGTGCTCGAGCTGCAAGAAGGAAGTCGAGGTGCCGCGCGATGCGTTGCTCAAGGAAGGCTTCCCC
GAGGAAAGCATCGGTACTTTCAAGATATACGGGCCGGTAGGTTGCGAAAACTGTGTGGGCGGTTACAAAGGGCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCAGCATTGGCCCGGATTATCATGGAGGACGGCAACTCAATCGATATCTCCACCC
AGATGCGCAAGGATGGCTTCAACGACTTGCGGACTTCGGCGCTGATCAAGGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTCAACCGGGTGACAAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.574

99.471

0.593
  pilB Acinetobacter baylyi ADP1

59.325

99.295

0.589
  pilB Legionella pneumophila strain ERS1305867

54.545

100

0.55
  pilB Vibrio cholerae strain A1552

51.264

97.707

0.501
  pilF Neisseria gonorrhoeae MS11

49.201

99.295

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

49.376

98.942

0.489
  pilB Vibrio campbellii strain DS40M4

48.936

99.471

0.487
  pilB/pilB1 Synechocystis sp. PCC 6803

36.201

100

0.393
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.983

93.651

0.365

Multiple sequence alignment