Detailed information NTG0001410

Experimentally validated

Overview

• Name: pilB/pilB1
• Type: Machinery gene
• Locus tag: SGL_RS13950
• Genome accession: NC_000911
• Coordinates: 2589851..2591869 (+)
• Length: 672 a.a.
• NCBI ID: WP_010873519.1
• Uniprot ID: -
• Organism: Synechocystis sp. PCC 6803
• Function: type IV pilus biogenesis and function DNA binding and uptake

Function

Competency of the natural transformation was lost in the slr0063, slr1274, slr1275, slr1276, slr1277 and sll1694 mutants and slr0197-disruptant. It was suggested that both DNA uptake for natural transformation and motility are mediated by a specific type IV-like pilus structure, while a putative DNA-binding protein encoded by slr0197 is additionally required for the DNA uptake.

Genomic Context

Location: 2584851..2596869

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SGL_RS13935 (sll0060)	-	2585489..2586523 (-)	1035	WP_010873516.1	AI-2E family transporter	-
SGL_RS13940 (sll0058)	dnaK	2586572..2588650 (-)	2079	WP_010873517.1	molecular chaperone DnaK	-
SGL_RS13945 (sll0057)	grpE	2588884..2589633 (-)	750	WP_010873518.1	nucleotide exchange factor GrpE	-
SGL_RS13950 (slr0063)	pilB/pilB1	2589851..2591869 (+)	2019	WP_010873519.1	GspE/PulE family protein	Machinery gene
SGL_RS13955 (slr0064)	-	2591953..2593107 (+)	1155	WP_010873520.1	RNA methyltransferase	-
SGL_RS13960 (slr0065)	-	2593329..2593967 (+)	639	WP_010873521.1	class I SAM-dependent methyltransferase	-
SGL_RS13965 (slr0066)	ribD	2594055..2595161 (+)	1107	WP_010873522.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-
SGL_RS19035	-	2595112..2595264 (-)	153	WP_071822661.1	DUF2256 domain-containing protein	-
SGL_RS13970 (sll0055)	-	2595280..2596566 (-)	1287	WP_010873523.1	M16 family metallopeptidase	-

Sequence

Protein

• Length: 672 a.a.
• Molecular weight: 75111.64 Da
• Isoelectric Point: 5.2598

>NTDB_id=1410 SGL_RS13950 WP_010873519.1 2589851..2591869(+) (pilB/pilB1) [Synechocystis sp. PCC 6803]
MTSSSSSKKSRAVALRNYFSPFGNKLVAGGHVDAEQLRQALVQVKKTGRSLPEELKAVTGRELSPELLRQYKKNQLFELK
VLYGVDSVDPEVAPIATQQMAELIGRFFPLDTCRRFKFLPLAQQEGDPPSVLVAMVDPDNLAAQDELNRILRVKGFELRR
LVVTQDDFNQLLEQYYAIREEWEAAQEKRDQERALDKLNDLTDIVGSIDMNLGEDKEDVDNALDSNDANQAPVINLVNKI
LAKALQEGTSDIHVEPQEETLRIRFRKDGVLNQAFDPLPRKITPAVVARFKIMADMDIAERRSPQDGKIRRIYQGRKVDF
RVNTLPSRYGEKVCLRILDNSATQLGLDFLITNPETLQTVRELAARPYGLMLVTGPTGSGKSTTLYSVLAERNSPEVNIN
TAEDPIEYALPGITQVQVIREKGMDFSNILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIAR
LDEMGVEPFMVSGSLLGVLAQRLMRKVCTECRIAYHPSKEELSRFGLSASGDEVVTFYKANSLSPDEIHLARQKGNLCQK
CSGSGYKGRVGVYEVMRNSERIAELINQGATTDRIKDCAVEEGMITLLAYSLNLVQEGYTTLEEVERVTFTDTGLESEMR
AKRKSALTCRTCAAELQQEWLDCPYCMTSRFS

Nucleotide

• Length: 2019 bp

>NTDB_id=1410 SGL_RS13950 WP_010873519.1 2589851..2591869(+) (pilB/pilB1) [Synechocystis sp. PCC 6803]
ATGACATCTTCCTCCTCTTCCAAGAAAAGCCGTGCCGTTGCCCTACGAAATTATTTTTCCCCCTTCGGCAATAAATTGGT
GGCGGGGGGCCATGTAGATGCTGAACAATTGCGACAGGCCCTAGTCCAGGTGAAAAAAACTGGGCGGTCTTTGCCCGAGG
AACTTAAGGCAGTCACTGGCCGAGAATTGTCCCCAGAACTACTGCGGCAGTACAAAAAAAATCAGTTATTTGAGCTCAAA
GTACTTTACGGGGTGGACAGCGTCGATCCGGAGGTTGCCCCGATCGCCACCCAGCAAATGGCCGAGTTGATTGGGCGATT
TTTTCCTTTGGATACTTGTCGGCGATTCAAGTTTTTGCCCTTAGCCCAGCAGGAAGGCGACCCCCCCAGTGTGCTAGTGG
CCATGGTGGACCCCGATAACTTGGCTGCCCAGGACGAGCTAAACCGAATTTTGCGGGTTAAAGGTTTTGAATTGCGCCGC
TTGGTGGTCACCCAAGATGATTTTAACCAACTGTTGGAACAGTACTATGCCATCCGGGAAGAGTGGGAGGCGGCCCAGGA
AAAAAGGGACCAGGAAAGGGCCCTTGATAAACTTAATGATTTGACGGATATTGTCGGCTCCATCGACATGAATCTGGGGG
AGGACAAAGAAGATGTAGATAATGCTCTAGACTCCAACGATGCTAACCAAGCACCGGTGATTAACCTGGTCAACAAAATT
TTGGCTAAGGCTCTCCAGGAAGGAACTTCCGACATTCACGTGGAGCCGCAGGAAGAAACCCTGAGAATTCGTTTCCGCAA
GGATGGGGTTTTAAATCAGGCTTTCGATCCCCTGCCCCGCAAAATTACCCCCGCCGTGGTGGCCCGTTTTAAAATTATGG
CGGACATGGACATTGCGGAGAGGCGATCGCCCCAGGATGGCAAAATTCGCCGTATTTACCAGGGCAGGAAGGTGGATTTT
CGGGTTAATACCCTACCCAGCCGCTATGGGGAAAAGGTTTGTTTGCGGATTTTGGACAACTCCGCCACTCAGTTGGGCTT
AGATTTTCTAATCACTAACCCCGAAACCCTACAAACGGTAAGGGAATTGGCCGCCCGGCCCTATGGCTTAATGTTGGTGA
CGGGGCCCACCGGATCGGGAAAATCCACCACCCTCTACTCGGTGTTGGCGGAACGCAATAGCCCCGAGGTGAATATCAAC
ACAGCGGAGGACCCGATTGAGTATGCTCTACCGGGCATTACCCAAGTGCAGGTGATTCGGGAAAAGGGCATGGATTTTTC
CAATATTCTGCGGGCATTTATGCGTCAGGATCCCGATGTGATTCTGGTGGGGGAAACCAGGGATAAGGAGACGGCCAAAA
CGGCCATTGAGGCGGCTCTGACTGGACACTTAGTTCTCACTACTCTCCACACCAATGATGCGGCCGGGGCGATCGCCAGA
TTGGATGAAATGGGAGTGGAGCCGTTTATGGTTTCTGGGTCATTGTTGGGGGTGTTGGCCCAACGGCTGATGCGGAAAGT
TTGTACGGAATGTCGCATTGCTTACCACCCTAGCAAGGAAGAATTGTCCCGCTTTGGGCTGTCCGCTTCCGGGGATGAAG
TGGTTACTTTTTATAAAGCCAATTCCCTCAGTCCCGACGAAATCCATCTTGCCCGTCAAAAAGGTAATTTATGTCAAAAA
TGTAGTGGCAGTGGCTATAAGGGTCGGGTGGGGGTCTATGAGGTGATGCGTAATTCTGAGCGTATTGCTGAATTGATTAA
CCAGGGGGCCACCACTGACCGCATTAAGGATTGTGCGGTGGAAGAGGGCATGATCACCCTATTGGCCTATAGTCTTAATT
TAGTGCAGGAAGGCTACACCACCCTAGAGGAGGTGGAACGGGTCACTTTCACCGATACCGGACTGGAGTCAGAAATGCGG
GCTAAGCGCAAGAGTGCTTTAACCTGTCGTACGTGTGCGGCGGAACTGCAACAGGAATGGCTTGATTGTCCCTATTGCAT
GACTTCCCGGTTTAGCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (239-506)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (73-180)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Vibrio campbellii strain DS40M4	37.374	100	0.396
pilB	Vibrio cholerae strain A1552	36.65	100	0.393
pilB	Vibrio parahaemolyticus RIMD 2210633	37.099	100	0.392
pilB	Acinetobacter baumannii D1279779	35.179	100	0.379
pilB	Acinetobacter baylyi ADP1	34.084	100	0.372

References

• [1]: S Yoshihara et al. (2001) Mutational analysis of genes involved in pilus structure, motility and transformation competency in the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. Plant & Cell Physiology 42(1):63-73. [PMID: 11158445]