Detailed information NTG0001058

Experimentally validated

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ABD1_RS01615
• Genome accession: NC_020547
• Coordinates: 347445..349157 (-)
• Length: 570 a.a.
• NCBI ID: WP_001274986.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii D1279779
• Function: power the assembly of type IV pilus DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.

Genomic Context

Location: 342445..354157

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABD1_RS01585 (ABD1_02990)	-	342607..342933 (+)	327	WP_000099685.1	pyrimidine/purine nucleoside phosphorylase	-
ABD1_RS01590 (ABD1_03000)	rlmB	343035..343784 (+)	750	WP_001118149.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
ABD1_RS01595 (ABD1_03010)	-	343820..344734 (+)	915	WP_000075010.1	DMT family transporter	-
ABD1_RS01600 (ABD1_03020)	coaE	344731..345327 (-)	597	WP_000874125.1	dephospho-CoA kinase	-
ABD1_RS18470 (ABD1_03030)	pilD	345329..346189 (-)	861	WP_001152280.1	prepilin peptidase	Machinery gene
ABD1_RS01610 (ABD1_03040)	pilC	346189..347415 (-)	1227	WP_000279215.1	type II secretion system F family protein	Machinery gene
ABD1_RS01615 (ABD1_03050)	pilB	347445..349157 (-)	1713	WP_001274986.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ABD1_RS01620 (ABD1_03060)	tpiA	349448..350242 (+)	795	WP_000016939.1	triose-phosphate isomerase	-
ABD1_RS01625 (ABD1_03070)	secG	350255..350584 (+)	330	WP_000555570.1	preprotein translocase subunit SecG	-
ABD1_RS01645 (ABD1_03080)	rimP	351226..351750 (+)	525	WP_000777730.1	ribosome maturation factor RimP	-
ABD1_RS01650 (ABD1_03090)	nusA	351788..353272 (+)	1485	WP_000532248.1	transcription termination factor NusA	-

Sequence

Protein

• Length: 570 a.a.
• Molecular weight: 63371.76 Da
• Isoelectric Point: 5.2391

>NTDB_id=1058 ABD1_RS01615 WP_001274986.1 347445..349157(-) (pilB) [Acinetobacter baumannii D1279779]
MSALHTSPKFTGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVNLDGPTAQEDEEEAPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCLECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide

• Length: 1713 bp

>NTDB_id=1058 ABD1_RS01615 WP_001274986.1 347445..349157(-) (pilB) [Acinetobacter baumannii D1279779]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAACTGCATTAGATGCAGCGAAAAGAGCTAAGCAGGACACAGTCGCATATTTAATTGAAGAGGTTCATCTCTCCC
CTTCGTTATTAGCTGAAACAATTTCTGCTGAGTTTGCTGAACCATATTTCGATCTGGATGTTTATGACACCTCTCAGATT
CCTAAAGATCTGGTCGATCAGAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGGCAAATTTTATATGT
TGCGACCAGTAATCCAAGCAATATCGAAGCGATTGATGCAATTCGTTTTAATAGTAAATTGCTGGTTGAGCCTGTAATTG
TGGAGCACCATAAACTCGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGTAGCTTTGACTTCAATGATGAAGAGTTT
GATCTCGATGTTAATCTTGATGGTCCTACCGCTCAAGAAGATGAAGAAGAAGCTCCTCAAGGCGATGAAGCCCCGATTGT
TAAATATATTAATAAGTTATTAATTGATGCCATTCGTATGGGTGCTTCAGACTTACATTTTGAACCGTACGAAAAATCCT
ATCGGGTGCGCTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCCCCTCTACAGCTTGCAAACCGCTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGTGTCCCCCAAGATGGTCGTATCAAGCTCAAGCTGTCAAA
AAGCAAAGCGATTGATTTTCGTGTGAACTCCCTCCCGACTTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGAATTGATGCACTAGGATATGAAGAAGATCAAAAAGCTTTGTTTATGGAGGCATTAGATAAACCT
CAAGGCATGCTTTTAATTACCGGACCAACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGACCCAGTAGAAATTAACCTTGAAGGAATTAATCAAGTTAACGTAAACCCCA
AGGTAGGCCTTACTTTTGCGGCCGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCGGCTCAAACTGGCCACATGGTGATGTCAACACTACATACTAATAGTGC
TCCGGAAACATTGACCCGTCTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAACCTAGTTATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGCAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCGCACCCAGATTTTCGGGTTTTTCAGCCCGTAGGTTGCCTTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCAGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTCAACCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (192-459)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (46-151)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	79.825	100	0.798
pilF	Neisseria gonorrhoeae MS11	50.088	100	0.509
pilB	Legionella pneumophila strain ERS1305867	50.794	99.474	0.505
pilB	Vibrio parahaemolyticus RIMD 2210633	51.698	94.474	0.488
pilB	Vibrio campbellii strain DS40M4	50.867	92.513	0.471
pilB	Vibrio cholerae strain A1552	50.29	91.993	0.463
pilB	Haemophilus influenzae Rd KW20	39.709	100	0.412
pilB	Glaesserella parasuis strain SC1401	36.187	100	0.403
pilB	Haemophilus influenzae 86-028NP	38.669	100	0.401
pilB/pilB1	Synechocystis sp. PCC 6803	35.179	100	0.379
pilF	Thermus thermophilus HB27	37.011	98.596	0.365

References

• [1]: Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB₂. Frontiers in Microbiology 12:738034. [PMID: 35126321]